| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 465,181 | C→A | 15.2% | V123F (GTC→TTC) | queC ← | 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 465,181 | 0 | C | A | 15.2% | 187.5 / 12.8 | 65 | V123F (GTC→TTC) | queC | 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base C (27/27); new base A (4/6); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATA > NC_000913/465086‑465278 | aaTATCTATCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGcc < 1:448009/99‑1 (MQ=255) atatCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAg > 1:2069814/1‑100 (MQ=255) tatCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGt < 1:512676/100‑1 (MQ=255) atCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGTGGCAATCCGGGTAGTCGGAGAAATCCGTTTCGCAGACGCCAg < 2:819149/98‑1 (MQ=255) tCTTTTGTCATGCGCAATCTGACGGCATGGTGTAGTTCTTTCACCAACTTATCTCGGCAATCCGTGTAGCCTGAGAAATCCGTTTCCCCGAc < 2:2402703/92‑1 (MQ=255) tCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAc < 2:1565151/100‑1 (MQ=255) cGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑g < 2:166989/100‑1 (MQ=255) cGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑g < 1:1555973/100‑1 (MQ=255) gCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTActg > 1:750275/1‑97 (MQ=255) ccATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGc < 1:1936299/100‑1 (MQ=255) cATGCCCAAACTGACGGCATGGTTTAGTGCTTGCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCt < 1:178290/100‑1 (MQ=255) gCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCt > 2:2381778/1‑100 (MQ=255) gCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCt > 1:2041466/1‑100 (MQ=255) gCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGCGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCt < 2:1327206/100‑1 (MQ=255) ccAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGTGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGc < 2:101101/100‑1 (MQ=255) ccAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGc > 1:1473044/1‑100 (MQ=255) ccAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGc < 1:501558/100‑1 (MQ=255) cAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCt < 2:1508664/100‑1 (MQ=255) cTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTa > 2:1662080/1‑100 (MQ=255) cTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaac < 2:634951/100‑6 (MQ=37) cTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaac < 2:750267/100‑6 (MQ=37) cTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCCTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaac < 1:1687736/100‑6 (MQ=25) tGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaacg < 2:541909/100‑7 (MQ=25) tGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaacg < 2:892808/100‑7 (MQ=25) gACGGCATGGTTTAGTGCTTTCACAAGCTCATCGCGGCACTCCGGGTAGCCGGAGAATTCCGTTTCGCAGGCGCGAGTAATTTC‑GGCTTCTGCTTTTAcc < 2:2133464/100‑1 (MQ=255) gACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTAcc > 2:1187561/1‑100 (MQ=255) gACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcgaaacgg < 2:499508/100‑8 (MQ=25) cgtcATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCTTGGTAGCCGGAGAATTCCGTTTTGCATATGCTATTAATTAC‑GGCTTCTGCTTTTACCt < 2:1064622/96‑1 (MQ=255) cGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTg > 2:2398029/1‑100 (MQ=255) ggCATGGTTTAGTGCTTTCACAAACTTATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGa < 2:847538/100‑1 (MQ=255) gCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGTGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTCATTAC‑GGCTTCTGCTTTTACCTGAt > 1:1663405/1‑100 (MQ=255) gCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGAt > 1:1516820/1‑100 (MQ=255) aTGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATa < 1:680142/99‑1 (MQ=255) ggTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGc < 2:51510/100‑1 (MQ=255) tttAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCat > 2:574407/1‑100 (MQ=255) tGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATAtt < 1:41876/100‑1 (MQ=255) cTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTgc > 1:1114671/1‑100 (MQ=255) tttCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTgc > 1:1230772/1‑99 (MQ=255) caAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCa > 1:1035638/1‑99 (MQ=255) aaCTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCg < 2:1762257/100‑1 (MQ=255) cTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTc < 2:1132302/100‑1 (MQ=255) aTCGCGGCAATCCGGGTATCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAgg > 1:559612/1‑100 (MQ=255) tCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGa < 1:1047925/100‑1 (MQ=255) gcgGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAAc > 1:986214/1‑100 (MQ=255) tCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAatat > 2:1712957/1‑100 (MQ=255) tCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAatat > 2:905054/1‑100 (MQ=255) ccGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTg > 1:1733163/1‑100 (MQ=255) ggAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTggg < 1:633562/100‑1 (MQ=255) gagaAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGa > 1:1840180/1‑100 (MQ=255) aTCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAAc > 2:1742210/1‑100 (MQ=255) tCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGAaaaa > 1:187484/1‑98 (MQ=255) tCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACg < 2:2022468/100‑1 (MQ=255) tCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGCAACAAAATATTACGCCCTGGGACAAACg < 2:2176409/100‑1 (MQ=255) ccGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGt < 2:1404066/100‑1 (MQ=255) gtaaaagCAGAAGCC‑GTAATTACTGGAGTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTa > 2:2211527/7‑100 (MQ=25) cGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTa > 2:183398/1‑100 (MQ=255) gTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCGTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTAt < 2:1995396/100‑1 (MQ=255) aaaagCAGAAGCC‑GTAATTACTGGCGTCTGCTTTTACCTGATACGCATATATTGCCGCCCGCGTCAGGAACAAAATATTACGCTCTGGCGCAAACGtct > 2:867721/5‑97 (MQ=21) ttCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTc > 2:2228247/1‑100 (MQ=255) ttCGCAGACACCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTc > 2:1501866/1‑100 (MQ=255) cGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCgg > 2:281701/1‑100 (MQ=255) cGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCgg > 1:919123/1‑100 (MQ=255) agCAGAAGCC‑GTAATTACTGGCGTCTGCTTTTACCTGATACGCATATTTTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCgg > 2:54322/2‑100 (MQ=37) gCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCggg < 1:905065/100‑1 (MQ=255) cAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGa > 1:409562/1‑100 (MQ=255) gACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATa < 2:986203/100‑1 (MQ=255) | AATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATA > NC_000913/465086‑465278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |