Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	520,993	T→G	100%	V193G (GTC→GGC)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	520,993	0	T	G	100.0%	255.8 / NA	74	V193G (GTC→GGC)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/34);  total (40/34)

CGGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGG  >  NC_000913/520897‑521089                                                                                                 |                                                                                                 cGGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCAc                                                                                               >  2:153168/1‑100 (MQ=255)       ccGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCg                                                                                         <  1:1110581/100‑1 (MQ=255)       ccGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCg                                                                                         >  1:1116479/1‑100 (MQ=255)       ccGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGGCGGGGCAGCTgg                                                                                           >  2:1311326/1‑98 (MQ=255)        cGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGt                                                                                        <  2:1198597/100‑1 (MQ=255)         ggTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGtt                                                                                       <  2:1250292/100‑1 (MQ=255)           ttttAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTtat                                                                                     <  1:846275/100‑1 (MQ=255)             ttAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATaa                                                                                   >  1:1013833/1‑100 (MQ=255)             ttAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATaa                                                                                   <  2:1104618/100‑1 (MQ=255)              tAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATaaa                                                                                  >  1:1680380/1‑100 (MQ=255)               aaCCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAAt                                                                                 >  2:1513212/1‑100 (MQ=255)               aaCCCCTTCCAGATAGCTCCGCGACTGATGATGAATCTGGCGGATGTATATAAAACCGCAGCCGTGCAACCGGGGACTCGGGGCACCTGGCGTTATACAt                                                                                 >  2:449888/1‑100 (MQ=255)                aCCCCTTCCGGCTGGCTCCACGTCAGATAATGAATCCGCCGAATGTCGATAAAACGGGAGGCGTGCAGCCAGGGAGTCGGGGCGCCTGGCGTTATAAAt                                                                                 >  2:278130/1‑99 (MQ=255)                aCCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAAtt                                                                                >  1:887617/1‑100 (MQ=255)                aCCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAAtt                                                                                >  1:610008/1‑100 (MQ=255)                aCCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAAtt                                                                                >  1:863083/1‑100 (MQ=255)                aCCCCTTCCAGATGGCTCCGCATCTGATGATGAATCTGGCGAATGTCGATAAAACCGGAGCCGTACAGCCGGGGAGTCGGGGCAACTGGCGTTATAAAt                                                                                 <  2:1673721/99‑1 (MQ=255)                 ccccTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTc                                                                               >  2:1626020/1‑100 (MQ=255)                  cccGTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcg                                                                              <  2:1603938/100‑1 (MQ=255)                    cTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcggc                                                                            <  2:370063/100‑1 (MQ=255)                      tCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcggcgg                                                                          <  1:2056738/100‑1 (MQ=255)                      tCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcggcgg                                                                          <  1:542786/100‑1 (MQ=255)                        cAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCa                                                                        >  1:2039125/1‑100 (MQ=255)                        cAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCa                                                                        >  1:427222/1‑100 (MQ=255)                            tGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACga                                                                    <  1:2338246/100‑1 (MQ=255)                             ggCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACgag                                                                   <  2:1510526/100‑1 (MQ=255)                               cTCCGCGTCTGATGATGAATCTGGCGGATGTCGATATAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGaa                                                                 >  1:1687165/1‑100 (MQ=255)                               cTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAACTTCGGCGGCAACGCGaa                                                                 >  1:1094554/1‑100 (MQ=255)                                tCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTACAAATTCGGCGGCAACGAGAAc                                                                >  2:1391790/1‑100 (MQ=255)                                 ccGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAAcn                                                               <  2:856871/100‑2 (MQ=255)                                 ccGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAAcc                                                               <  2:1067063/100‑1 (MQ=255)                                   gcgTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAg                                                             <  1:520389/100‑1 (MQ=255)                                      tCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTc                                                          <  2:628475/100‑1 (MQ=255)                                          atgatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACg                                                      >  1:861056/1‑100 (MQ=255)                                            gatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGc                                                    >  1:1563994/1‑100 (MQ=255)                                                 aTCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATga                                               <  1:1770855/100‑1 (MQ=255)                                                  tCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATgag                                              >  2:665123/1‑100 (MQ=255)                                                   cTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGTGGCAACGAGAACCAGCTCGACGGCTATgaga                                             <  1:1597769/100‑1 (MQ=255)                                                     ggCGGATGTCGATAAAACCGGAGCCGTGCAGGCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATga                                               <  2:864822/96‑1 (MQ=255)                                                     ggCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGaaa                                           >  1:525155/1‑100 (MQ=255)                                                      gCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAAt                                          >  1:1303202/1‑100 (MQ=255)                                                        ggATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATgg                                        <  2:169669/100‑1 (MQ=255)                                                        ggATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATgg                                        >  1:2229232/1‑100 (MQ=255)                                                         gATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgt                                       <  2:2124184/100‑1 (MQ=255)                                                           tGTCGATAGAACCGGAGCGGTGCAGCCGGGTAGTCGGGGCATCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgttg                                     <  2:1136952/100‑1 (MQ=255)                                                           tGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgttg                                     <  1:1194522/100‑1 (MQ=255)                                                           tGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgttg                                     <  2:1699507/100‑1 (MQ=255)                                                           tGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgtt                                      <  1:179049/99‑1 (MQ=255)                                                             tCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCGATGAGAAATGgatgt                                    >  2:1099528/1‑99 (MQ=255)                                                               gATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTa                                  >  2:2297240/1‑99 (MQ=255)                                                                aTAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTAcc                                >  1:882437/1‑100 (MQ=255)                                                                aTAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTAcc                                <  2:506768/100‑1 (MQ=255)                                                                aTAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTAcc                                <  1:2141277/100‑1 (MQ=255)                                                                   aaaCCGGAGCGGTGCAGGCAGGGAGACGGGGCACATGGCGTTATAAATTCGGCGGCAACGAGAACCAGCACGGCGGCGCTGAGAAAGAGATGTTACcaca                             >  2:1108272/1‑100 (MQ=255)                                                                   aaaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCa                             >  1:220952/1‑100 (MQ=255)                                                                   aaaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCa                             <  2:2271116/100‑1 (MQ=255)                                                                   aaaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAACTCGACGGCTATGAGAAATGGTTGTTACCTCa                             >  1:1751703/1‑100 (MQ=255)                                                                    aaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGGTATGGGAGATGGTGGTGACCTCAg                            >  2:2002063/1‑100 (MQ=255)                                                                    aaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAg                            <  1:431149/100‑1 (MQ=255)                                                                    aaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGACGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAg                            >  1:1689034/1‑100 (MQ=255)                                                                     aCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTCTGAGAAATGGTTGATACCTCacc                           >  2:1241146/1‑98 (MQ=255)                                                                      ccGGAGCCGTGCAGACGGGTAGTAGGGGAACCTGAAGATATAAATTCGGGGGCAACGCTAACCAAATCGAAGGCTATGAGAAATGGTTGTTaggtaa                             >  2:722048/1‑92 (MQ=255)                                                                       cGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCt                          <  2:2296456/99‑1 (MQ=255)                                                                       cGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCt                          <  2:347646/99‑1 (MQ=255)                                                                              gTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCg                  >  1:2049535/1‑100 (MQ=255)                                                                              gTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAAAAATGGTTGTTACCTCAGCTTAAAccc                   >  1:1253576/1‑99 (MQ=255)                                                                               tGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGa                 >  1:808700/1‑100 (MQ=255)                                                                                 cAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaac               <  1:153171/100‑1 (MQ=255)                                                                                    ccGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaacaac            >  1:2366359/1‑100 (MQ=255)                                                                                     cGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACg           >  2:1232356/1‑100 (MQ=255)                                                                                     cGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACCGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGCACAACg           >  2:1536023/1‑100 (MQ=255)                                                                                          aGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCGGCTCGACGGCTATGAGAAATGGTTGTTACCTCATCTTAAACCCGAACAACGCTGGt      <  2:835647/100‑1 (MQ=255)                                                                                           gTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTa     <  2:863074/100‑1 (MQ=255)                                                                                             cGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACg   >  2:672770/1‑100 (MQ=255)                                                                                              ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACgg  >  1:725630/1‑100 (MQ=255)                                                                                              ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACgg  >  2:1880730/1‑100 (MQ=255)                                                                                              ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACgg  >  1:777520/1‑100 (MQ=255)                                                                                              ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGATGTTACCTCGGCTTACACCCGAACACCACTGGt      >  2:2329435/1‑96 (MQ=255)                                                                                                 |                                                                                                 CGGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGG  >  NC_000913/520897‑521089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑