Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 661,369 T→C 24.6% N4D (AAT→GAT)  ybeF ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913661,3690TC24.6% 163.9 / 55.6 97N4D (AAT→GAT) ybeFLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base C (12/12);  total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCACC  >  NC_000913/661272‑661468
                                                                                                 |                                                                                                   
aTAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTAc                                                                                                   <  1:1808856/100‑1 (MQ=255)
aTAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCAc                                                                                                   <  1:677044/100‑1 (MQ=255)
   gTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTAt                                                                                                <  2:677875/100‑1 (MQ=255)
   gTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTAt                                                                                                >  1:1599148/1‑100 (MQ=255)
   gTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTAt                                                                                                >  1:1538171/1‑100 (MQ=255)
      aGAAGGTTAAGATCAATATTTCGTACGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCa                                                                                             <  2:1555118/100‑1 (MQ=255)
      aGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCa                                                                                             >  1:1188026/1‑100 (MQ=255)
      aGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCa                                                                                             >  1:1663310/1‑100 (MQ=255)
         aGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGc                                                                                          <  2:1027775/100‑1 (MQ=255)
         aGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGc                                                                                          <  2:1298766/100‑1 (MQ=255)
          ngTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGGCGACTTTCTGCTTAAGCAGGGTTCAATTTGATTATTACTATCCACGc                                                                                          >  1:1119692/2‑99 (MQ=255)
          ggTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCa                                                                                         >  1:1446747/1‑100 (MQ=255)
           gTTAAGATCCATATTACGTAAGGTTGTAAATAACTGTGAATAACCTTCTGACTACTTTCTGCTTAAATAGGGTTCAATTTGATTATTACTATCCACGCAc                                                                                        >  2:828915/1‑100 (MQ=255)
           gTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCAc                                                                                        <  1:35423/100‑1 (MQ=255)
             tAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACtt                                                                                      >  1:1417152/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACt                                                                                   >  1:372984/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACt                                                                                   >  1:341221/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACt                                                                                   >  2:2136942/1‑100 (MQ=255)
                    aaTATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCaa                                                                               >  1:2331776/1‑100 (MQ=255)
                       atTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAAttt                                                                            <  1:95966/100‑1 (MQ=255)
                           cGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTAtt                                                                        >  1:1740863/1‑100 (MQ=255)
                            gTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTc                                                                       <  1:1242536/100‑1 (MQ=255)
                            gTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTc                                                                       <  1:1731802/100‑1 (MQ=255)
                             tAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCa                                                                      >  2:2360000/1‑100 (MQ=255)
                             tAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCa                                                                      <  1:1705404/100‑1 (MQ=255)
                               aGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATg                                                                    <  1:2345435/100‑1 (MQ=255)
                                ggTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACATGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATgg                                                                   <  2:515464/100‑1 (MQ=255)
                                 gTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGa                                                                  >  1:245288/1‑100 (MQ=255)
                                   tGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaa                                                                >  1:1308968/1‑100 (MQ=255)
                                   tGTAAATATTTGCGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGaaa                                                                >  1:75141/1‑100 (MQ=255)
                                    gTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGaaaa                                                               >  1:397531/1‑100 (MQ=255)
                                    gTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGaaaa                                                               >  2:6686/1‑100 (MQ=255)
                                     tAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGaaaaa                                                              <  1:2202198/100‑1 (MQ=255)
                                      aaaTATTTGTTGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                             <  1:1677671/100‑1 (MQ=255)
                                      aaaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                             <  2:2207253/100‑1 (MQ=255)
                                        atatTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaataa                                                           >  1:189730/1‑100 (MQ=255)
                                        atatTTGTAGTTTACCTTCTGACGACTTTCTGCTTAAACATGATTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaata                                                            <  2:1701845/99‑1 (MQ=255)
                                          atTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAataata                                                         <  1:6686/100‑1 (MQ=255)
                                          atTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAataata                                                         <  1:1422517/100‑1 (MQ=255)
                                           tttGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAatat                                                        >  2:1746707/1‑100 (MQ=255)
                                             tgtgGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATAttt                                                      <  2:1058162/100‑1 (MQ=255)
                                             tgtgGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATAttt                                                      <  2:145229/100‑1 (MQ=255)
                                             tgtgGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATAttt                                                      >  2:580055/1‑100 (MQ=255)
                                                    tACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAt                                               >  1:822910/1‑100 (MQ=255)
                                                    tACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAt                                               <  2:161307/100‑1 (MQ=255)
                                                     aCCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAtt                                              >  2:315587/1‑100 (MQ=255)
                                                      ccTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTa                                             >  1:1094483/1‑100 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTAATATCCACGTACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTa                                             <  2:1734256/99‑1 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                            <  1:720655/100‑1 (MQ=255)
                                                        ttttGACGACTTTCTGCTTAAACAGGTTTCAATTTGATTATTACTATCTACGCACTTACTCCCCTTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                            <  2:805985/96‑1 (MQ=255)
                                                        ttatGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                            <  2:1314579/96‑1 (MQ=255)
                                                        ttCTGACGACTTTCTGCTTAAACAGGGTTCAATTTTATTATTACTATAAACGCACTTACTCCAATATTAAAAATAAAAAAATAATATTTAAAAAATTAc                                            <  2:154888/99‑1 (MQ=255)
                                                        ttCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACa                                           >  1:1643496/1‑100 (MQ=255)
                                                          cTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCCCGCACTTACTTCAATTTTATTTATGGAAAAATAATATTTAAAAACTTACaat                                         >  2:2393121/1‑100 (MQ=255)
                                                          cTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaat                                         <  1:45071/100‑1 (MQ=255)
                                                          cTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaat                                         >  2:1269683/1‑100 (MQ=255)
                                                          cTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaa                                          >  1:15533/1‑99 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                       <  1:584346/100‑1 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGGTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                       >  2:1797887/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                     <  1:1906018/100‑1 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                     <  1:2360028/100‑1 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataat                                      >  1:258931/1‑99 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                                  >  2:190038/1‑100 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                                  >  2:339910/1‑100 (MQ=255)
                                                                  ttttTGCTTAAACATGGTTTAATTTGATTATTATTATTTACGTATTTATTTTAATTTTATTCATGGTAAAATAATATTTAAAAAATTACAATAATCtt                                   <  2:620602/98‑1 (MQ=255)
                                                                  tttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTAt                                 >  1:1043583/1‑100 (MQ=255)
                                                                     cTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTc                              <  2:1335670/100‑1 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCt                             <  2:276453/100‑1 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                            >  2:2288534/1‑100 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                            >  1:983985/1‑100 (MQ=255)
                                                                        cTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTaa                           <  2:2253132/100‑1 (MQ=255)
                                                                          tAAACAGTATTAAATTTGATTATTACTATCCACGCACTTACTCCAATAAAAAAAAAAAAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAAtt                         <  2:1086113/100‑1 (MQ=255)
                                                                            aaCAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTATACAATTACAATAATCTTATGTCTAAt                          >  2:360053/1‑97 (MQ=255)
                                                                            aaCAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTgg                       >  2:1018378/1‑100 (MQ=255)
                                                                               aGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaac                    <  2:2398847/100‑1 (MQ=255)
                                                                                 ggTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgg                  <  2:660410/100‑1 (MQ=255)
                                                                                  gTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgga                 >  2:1141869/1‑100 (MQ=255)
                                                                                  gTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgga                 <  2:2231861/100‑1 (MQ=255)
                                                                                  gTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgga                 <  2:1242199/100‑1 (MQ=255)
                                                                                     cAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGgaacggaacg              >  2:186400/1‑100 (MQ=255)
                                                                                     cAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGgaacggaacg              >  2:1364315/1‑100 (MQ=255)
                                                                                     cAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGgaacggaacg              >  2:2032000/1‑100 (MQ=255)
                                                                                        tttGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCt            >  2:2381701/1‑99 (MQ=255)
                                                                                         ttGATTATTACTATCCACGCACTTACTCCAATATTAAACATAAAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCttt          <  2:2301292/100‑1 (MQ=255)
                                                                                          tgtttattaCTATCCATGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtttt         <  2:1400532/97‑1 (MQ=255)
                                                                                          tGATTATTACTATCCACTCTCTTACTCTAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtttt         <  1:226366/100‑1 (MQ=255)
                                                                                           gATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg        >  2:1210592/1‑100 (MQ=255)
                                                                                           gATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAATATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg        <  2:1308954/100‑1 (MQ=255)
                                                                                           gATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg        <  2:867219/100‑1 (MQ=255)
                                                                                            attattACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGc       <  1:485734/100‑1 (MQ=255)
                                                                                             ttattaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCt      <  2:341218/100‑1 (MQ=255)
                                                                                             ttatcaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtttt         >  2:1880824/1‑97 (MQ=255)
                                                                                             ttatcaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCt      >  1:423597/1‑100 (MQ=255)
                                                                                              tattaCTATTCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTc     <  2:1063739/100‑1 (MQ=255)
                                                                                               attaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCa    <  1:1436067/100‑1 (MQ=255)
                                                                                               attaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCa    <  1:1633458/100‑1 (MQ=255)
                                                                                                ttattaACCACGCAAATACTTCAATTTTATTCATGGAAAAATAAAAATAAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCAc   <  1:765866/100‑1 (MQ=255)
                                                                                                ttaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCAc   >  2:1496314/1‑100 (MQ=255)
                                                                                                 taCTATCCACTCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCAcc  <  2:1090435/100‑1 (MQ=255)
                                                                                                 taCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCAcc  <  2:232883/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTCACC  >  NC_000913/661272‑661468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: