Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	674,180	G→A	71.3%	L202L (CTG→TTG)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,180	0	G	A	71.3%	104.4 / 58.9	80	L202L (CTG→TTG)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (13/10);  new base A (25/32);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

TGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTT  >  NC_000913/674081‑674278                                                                                                    |                                                                                                   tGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGagcag                                                                                                    >  1:2398239/1‑100 (MQ=255)    aGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTc                                                                                                 <  1:1793012/100‑1 (MQ=255)     ggTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTc                                                                                                 >  1:230093/1‑99 (MQ=255)     ggTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCg                                                                                                <  2:1720941/100‑1 (MQ=255)      gTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGt                                                                                               <  2:2119898/100‑1 (MQ=255)        gATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCa                                                                                             >  2:982886/1‑100 (MQ=255)        gATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCa                                                                                             >  1:627052/1‑100 (MQ=255)         aTCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAg                                                                                            >  1:1025650/1‑100 (MQ=255)          tctcCACGCCTTCGGAATGACCGATCCAGTTACGCTGCATGGTTTTAACTGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCa                                                                                             <  2:209413/98‑1 (MQ=255)          tctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGc                                                                                           <  2:1445408/100‑1 (MQ=255)           ctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCg                                                                                          <  2:72978/100‑1 (MQ=255)           ctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCg                                                                                          <  1:2352324/100‑1 (MQ=255)             ccACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGTTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTa                                                                                        <  2:934466/100‑1 (MQ=255)               aCGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAg                                                                                      <  2:950229/100‑1 (MQ=255)                cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGc                                                                                     >  1:1449421/1‑100 (MQ=255)                   cTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTaagaag                                                                                   >  1:650480/1‑99 (MQ=255)                   cTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCAGGCCAGTGATCCAGTTTATCCAGATCGTTGAGAAACTCGTCAGCGTAAGCAg                                                                                   >  2:1324549/1‑99 (MQ=255)                    ttCGGAAGGACAGATCCAGTTACTCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAg                                                                                   <  2:541382/98‑1 (MQ=255)                    ttCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTg                                                                                 <  2:892556/100‑1 (MQ=255)                     tCGGACAGACCGATCGAGTTAAGATAAATCGTTTCCACGGCCTCTGCACCATCATCCATTTTATCCAGATCGTTGAGCAACTCACCAGCGTa                                                                                        <  2:1427038/92‑1 (MQ=255)                     tCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGa                                                                                >  1:1140593/1‑100 (MQ=255)                       ggAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAtt                                                                              <  1:728130/100‑1 (MQ=255)                        gAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAttt                                                                             >  1:842991/1‑100 (MQ=255)                        gAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAttt                                                                             >  1:2126666/1‑100 (MQ=255)                         aaCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAtttt                                                                            <  1:1416088/100‑1 (MQ=255)                          aCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTg                                                                           <  2:2391079/100‑1 (MQ=255)                           cGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGa                                                                          <  1:164386/100‑1 (MQ=255)                           cGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGa                                                                          <  2:694915/100‑1 (MQ=255)                             actgATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGAGCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATa                                                                        <  2:1669030/97‑1 (MQ=255)                             aCCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATa                                                                        <  1:1064371/100‑1 (MQ=255)                             aCCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATa                                                                        >  1:236921/1‑100 (MQ=255)                                gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAAc                                                                     >  1:528448/1‑100 (MQ=255)                                gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAAc                                                                     >  2:1919000/1‑100 (MQ=255)                                gATCAAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAAc                                                                     >  2:625912/1‑100 (MQ=255)                                 aTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAAcc                                                                    >  1:1393119/1‑100 (MQ=255)                                  tCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCa                                                                   >  2:1951305/1‑100 (MQ=255)                                   ccAGTTACGCTGCATGGTTTTAACGGTTTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAAcc                                                                    <  2:1320280/98‑1 (MQ=255)                                   ccAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCAc                                                                  >  2:2263763/1‑100 (MQ=255)                                    cAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACt                                                                 >  2:746022/1‑100 (MQ=255)                                     aGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTg                                                                >  1:1334724/1‑100 (MQ=255)                                     aGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTg                                                                >  1:1815371/1‑100 (MQ=255)                                      gTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGc                                                               >  1:876477/1‑100 (MQ=255)                                      gTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGc                                                               >  1:1996234/1‑100 (MQ=255)                                        tACTCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCgg                                                             <  2:525911/100‑1 (MQ=255)                                         aCGCTGCATGGTTTTAACGGTGTCTGGACAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCggg                                                            <  2:539377/100‑1 (MQ=255)                                            cTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGAtc                                                         >  1:609433/1‑100 (MQ=255)                                            cTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGAtc                                                         <  2:1025639/100‑1 (MQ=255)                                               cATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATctct                                                      >  1:1172082/1‑100 (MQ=255)                                                aTGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCtt                                                     >  1:1810236/1‑100 (MQ=255)                                                aTGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGAAGTGATTTTGATAAACCACTGCGGGATCTCtt                                                     >  2:159643/1‑100 (MQ=255)                                                   gTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTAc                                                  >  1:2259635/1‑100 (MQ=255)                                                    ttttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACg                                                 <  1:866205/100‑1 (MQ=255)                                                        aaCGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCa                                             <  1:1317050/100‑1 (MQ=255)                                                        aaCGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCa                                             <  2:2126642/100‑1 (MQ=255)                                                           ggTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACt                                          <  2:492142/100‑1 (MQ=255)                                                               tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                      >  2:937680/1‑100 (MQ=255)                                                               tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                      <  1:2386345/100‑1 (MQ=255)                                                               tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                      <  1:1216777/100‑1 (MQ=255)                                                                cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGt                                     >  1:2028586/1‑100 (MQ=255)                                                                cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTg                                       >  1:73668/1‑98 (MQ=255)                                                                cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGt                                     <  1:1362341/100‑1 (MQ=255)                                                                  ggCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTAt                                   >  1:696655/1‑100 (MQ=255)                                                                   gCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTAt                                   >  1:1276247/1‑99 (MQ=255)                                                                   gCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATc                                  <  1:209507/100‑1 (MQ=255)                                                                     cAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCg                                 <  1:1362375/99‑1 (MQ=255)                                                                       gTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAg                              >  1:1202805/1‑100 (MQ=255)                                                                       gTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAg                              >  1:1042787/1‑100 (MQ=255)                                                                         gATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAgcg                            <  1:21251/100‑1 (MQ=255)                                                                         gATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAgcg                            <  2:696647/100‑1 (MQ=255)                                                                           tCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGcc                          >  1:793484/1‑100 (MQ=255)                                                                           tCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGcc                          <  1:1021729/100‑1 (MQ=255)                                                                            ccAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCca                         >  1:1126862/1‑100 (MQ=255)                                                                            ccAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCca                         >  1:311814/1‑100 (MQ=255)                                                                                ttgaTCCAGAGCGTTGAGCAACTCGTGAGCGTAAGGAGTGATTTTGATAAACCCCTGCGGGATCTCTTTTCGTTCAACTTTTATATCGCAgcg                            <  2:502461/90‑1 (MQ=255)                                                                                 ttATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagc                    <  1:571703/100‑1 (MQ=255)                                                                                 ttATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagc                    <  1:260199/100‑1 (MQ=255)                                                                                   aTCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagcag                  <  1:982897/100‑1 (MQ=255)                                                                                      cAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGcc                >  1:965479/1‑99 (MQ=255)                                                                                       aGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGt              <  2:1996212/100‑1 (MQ=255)                                                                                        gATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc             <  2:529232/100‑1 (MQ=255)                                                                                            gTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATa         <  1:921000/100‑1 (MQ=255)                                                                                                agcagcTCATCAGCGTAAGCAGCGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACt      <  2:196540/99‑1 (MQ=255)                                                                                                 gcagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACt      >  2:1084018/1‑98 (MQ=255)                                                                                                 gcagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTg    >  2:1041799/1‑100 (MQ=255)                                                                                                   agcTCGGCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTgtt  <  1:2229223/100‑1 (MQ=255)                                                                                                    |                                                                                                   TGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTT  >  NC_000913/674081‑674278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑