Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 135,281 A→T 16.4% L101Q (CTG→CAG)  speD ← S‑adenosylmethionine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913135,2810AT16.4% 246.4 / 38.9 103L101Q (CTG→CAG) speDS‑adenosylmethionine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (43/43);  new base T (8/9);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 104 total aligned reads displayed.

GCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGC  >  NC_000913/135187‑135380
                                                                                              |                                                                                                   
gcgGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTggg                                                                                                <  1:2268052/100‑1 (MQ=255)
   gAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGCCTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCg                                                                                             <  1:1073314/100‑1 (MQ=255)
   gAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCg                                                                                             >  1:449357/1‑100 (MQ=255)
      ggTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCgggg                                                                                          >  2:542159/1‑100 (MQ=255)
       gTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCgggg                                                                                          >  2:150361/1‑99 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACGGTTTCTGGCAGTGGGCCggg                                                                                           >  2:117274/1‑97 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGgtg                                                                                        >  2:742952/1‑100 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCgggg                                                                                          >  2:2095065/1‑98 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGgtg                                                                                        <  1:810441/100‑1 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGgtg                                                                                        >  2:673394/1‑100 (MQ=255)
        tACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGgtg                                                                                        >  2:426475/1‑100 (MQ=255)
         aCATAAACCGCCTTCAGGATGACTTTCCTGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGgtgt                                                                                       >  2:834452/1‑100 (MQ=255)
          cATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGtt                                                                                      >  1:1644696/1‑100 (MQ=255)
           aTAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTc                                                                                     >  1:1091694/1‑100 (MQ=255)
            tAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCt                                                                                    >  2:828608/1‑100 (MQ=255)
            tAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCt                                                                                    >  1:1212862/1‑100 (MQ=255)
              aaCCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGt                                                                                  <  1:271541/100‑1 (MQ=255)
                 cGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAAGATGACTTTTATCAACATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGtttt                                                                               <  1:811948/100‑1 (MQ=255)
                  gCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTg                                                                              >  1:1899779/1‑100 (MQ=255)
                  gCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTg                                                                              <  1:732479/100‑1 (MQ=255)
                   ccTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTGTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGt                                                                             >  2:2057151/1‑100 (MQ=255)
                    cTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTc                                                                            >  2:175895/1‑100 (MQ=255)
                     ttCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCg                                                                           <  2:1174709/100‑1 (MQ=255)
                     ttCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCg                                                                           <  1:464920/100‑1 (MQ=255)
                       cAGGATGACTTTTCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGAt                                                                         <  1:1210618/100‑1 (MQ=255)
                        tggATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATg                                                                        <  2:1645832/99‑1 (MQ=255)
                        aGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATg                                                                        <  2:148567/100‑1 (MQ=255)
                         ggATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGa                                                                       >  1:1645852/1‑100 (MQ=255)
                              aCTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTc                                                                  >  1:1878383/1‑100 (MQ=255)
                              aCTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTc                                                                  >  1:190502/1‑100 (MQ=255)
                               cTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 >  2:1822990/1‑100 (MQ=255)
                               cTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 >  2:404057/1‑100 (MQ=255)
                               cTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 >  2:826375/1‑100 (MQ=255)
                               cTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 >  2:1300030/1‑100 (MQ=255)
                               cTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 >  2:246894/1‑100 (MQ=255)
                                tttCCGGGTAGGTATGTACGGAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGTTTTTCgg                                                                >  1:521668/1‑100 (MQ=255)
                                tttCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCg                                                                 <  1:360428/99‑1 (MQ=255)
                                 ttCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCggg                                                               <  2:972973/100‑1 (MQ=255)
                                 ttCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCggg                                                               <  1:1094867/100‑1 (MQ=255)
                                   ccGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTc                                                             <  1:172430/100‑1 (MQ=255)
                                   ccGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTc                                                             >  2:528204/1‑100 (MQ=255)
                                          ggtaTGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGt                                                      >  1:737061/1‑100 (MQ=255)
                                          ggtaTGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGt                                                      <  2:161625/100‑1 (MQ=255)
                                             aTGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttct                                                   <  1:948540/100‑1 (MQ=255)
                                             aTGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGATCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttct                                                   <  1:1692952/100‑1 (MQ=255)
                                              tGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttctt                                                  <  2:644029/100‑1 (MQ=255)
                                              tGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttctt                                                  <  2:393397/100‑1 (MQ=255)
                                              tGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttctt                                                  <  1:673402/100‑1 (MQ=255)
                                               gTACGGAAATTTGATTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttcttc                                                 <  2:2089123/100‑1 (MQ=255)
                                               gTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttcttc                                                 >  2:2221270/1‑100 (MQ=255)
                                               gTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGttctgc                                                 <  2:894437/100‑3 (MQ=255)
                                                tACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtca                                                >  2:500890/1‑100 (MQ=255)
                                                 aCGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcac                                               <  1:663904/100‑1 (MQ=255)
                                                 aCGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcac                                               <  2:1555204/100‑1 (MQ=255)
                                                  cGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcact                                              >  2:2315722/1‑100 (MQ=255)
                                                  cGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcact                                              >  2:70945/1‑100 (MQ=255)
                                                    cAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcactca                                            >  2:79098/1‑100 (MQ=255)
                                                    cAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcactca                                            >  2:1962016/1‑100 (MQ=255)
                                                    cAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcactca                                            >  1:1341144/1‑100 (MQ=255)
                                                     aaaTATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTtcactc                                             <  2:1939760/98‑1 (MQ=255)
                                                      aaTATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCAcc                                          <  2:1878361/100‑1 (MQ=255)
                                                         atGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGa                                       >  2:1720136/1‑100 (MQ=255)
                                                          tGACTTTTATCAAGATTAGCAACGAACGATTAAGGAAGTGGGCCGAGGTGTACTGTCTAGTCTCTGAGTGTCGTCACAACCGGTTCTTCCCTCACCaaaa                                      >  2:433329/1‑100 (MQ=255)
                                                           gACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAAt                                     <  2:1759991/100‑1 (MQ=255)
                                                           gACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAAt                                     <  2:1992535/100‑1 (MQ=255)
                                                           gACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTAAACCGGTTCTTCACTCACTAGaa                                      >  2:110927/1‑99 (MQ=255)
                                                             cTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAg                                   >  2:2145861/1‑100 (MQ=255)
                                                               tttATCAAGATGGTCAACGGCTTTTTTTGGCAGTGGGCTGGGGTGTTCTGTTTTGTCGATTAGTTTTGGGTCAACCGGTTCTGCACGCAcc                                          <  2:342975/91‑1 (MQ=255)
                                                               tttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTg                                 <  1:1514849/100‑1 (MQ=255)
                                                               tttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTg                                 <  2:872522/100‑1 (MQ=255)
                                                                ttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGa                                <  1:2091948/100‑1 (MQ=255)
                                                                ttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGa                                <  2:2028123/100‑1 (MQ=255)
                                                                ttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGa                                <  1:2201346/100‑1 (MQ=255)
                                                                ttATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGa                                <  1:537338/100‑1 (MQ=255)
                                                                 tATCAAGATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGAc                               >  2:376418/1‑100 (MQ=255)
                                                                  aTCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACg                              >  1:1187236/1‑100 (MQ=255)
                                                                   tCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGc                             >  1:2307445/1‑100 (MQ=255)
                                                                    cAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCt                            >  2:1299897/1‑100 (MQ=255)
                                                                       gATGGGCAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGc                         <  2:1313800/100‑1 (MQ=255)
                                                                       gATGGGCAACGACCGTTTCTGGCTGGGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGc                         <  1:587823/100‑1 (MQ=255)
                                                                       gATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGc                         <  2:849882/100‑1 (MQ=255)
                                                                         tGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCAc                       >  1:275688/1‑100 (MQ=255)
                                                                          gagtaaCGACCGTTTCTGGCATTGTGACGAGGTTTTCTGTTTTCTCCATGCATATCGGTTAAACCGGTTCTTCTTTTACCAGAATAGTGACGCTGGCAct                      <  2:1329759/96‑2 (MQ=255)
                                                                           ggCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCAccc                     <  1:308526/100‑1 (MQ=255)
                                                                           ggCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCAccc                     <  1:2202821/100‑1 (MQ=255)
                                                                           ggCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCAccc                     >  1:1827755/1‑100 (MQ=255)
                                                                            ncAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCt                    >  1:728365/2‑100 (MQ=255)
                                                                            gCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCAcc                      >  2:566472/1‑98 (MQ=255)
                                                                            gCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCt                    <  1:958953/100‑1 (MQ=255)
                                                                            gCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCt                    >  2:171731/1‑100 (MQ=255)
                                                                            gCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCt                    >  2:1993353/1‑100 (MQ=255)
                                                                             cAACGACCGTTTCTGGCTGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCtg                   <  1:524982/100‑1 (MQ=255)
                                                                                     gTTTCTGGCAGTGGGCCGGGTTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGt           <  1:144760/100‑1 (MQ=255)
                                                                                     gTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGt           <  1:528207/100‑1 (MQ=255)
                                                                                     gTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTGTCGGTTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGt           <  2:1681471/100‑1 (MQ=255)
                                                                                      tttCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGt           <  2:407544/99‑1 (MQ=255)
                                                                                         cTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATc       <  1:2095087/100‑1 (MQ=255)
                                                                                           agCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCt     >  2:766011/2‑100 (MQ=255)
                                                                                            gCAGTTGGCTGGTGTGTTCTTTTTTGTCTATGAGTTTCGGCTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTg    <  2:1817896/100‑1 (MQ=255)
                                                                                              aGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTggc  >  2:744664/1‑100 (MQ=255)
                                                                                              |                                                                                                   
GCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTTGTCGATGAGTTTCGGGTCAACCGGTTCTTCACTCACCAGAATAGTGACGCTGGCACCCTGTGGTTCGTAATCCTGGC  >  NC_000913/135187‑135380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑