Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 772,209 | C→A | 100% | T251N (ACC→AAC) | cydA → | cytochrome d terminal oxidase, subunit I |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 772,209 | 0 | C | A | 97.1% | 220.0 / ‑0.8 | 70 | T251N (ACC→AAC) | cydA | cytochrome d terminal oxidase, subunit I |
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (30/37); total (32/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01 |
CTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACA > NC_000913/772119‑772304 | cTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgc < 1:1252651/100‑1 (MQ=255) cTATCGCTGCCAACTTCGGTATGGCGGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgc < 2:850450/100‑1 (MQ=255) tATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgct > 2:554097/1‑100 (MQ=255) cGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGgctgct < 2:125624/100‑1 (MQ=255) tGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAt > 1:1465971/1‑99 (MQ=255) gCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTg > 1:1252023/1‑100 (MQ=255) ccAGCTTCGGTATGGCTTCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGa < 1:2394423/100‑1 (MQ=255) ccAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGa < 1:981322/100‑1 (MQ=255) aGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAg < 1:77039/100‑1 (MQ=255) aGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAg < 2:1120212/100‑1 (MQ=255) aGCTTCGGTATGGCTGCTGTGCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAtt < 2:2381121/96‑1 (MQ=255) ttCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCg < 1:2355138/100‑1 (MQ=255) ggTATGGCTGCTGTTATGTCTGTTATAGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGt > 2:788318/1‑100 (MQ=255) tcttccgatctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTg < 1:1590142/91‑1 (MQ=255) tATGGCTGCTGTTCTGTCGGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg < 2:2310687/100‑1 (MQ=255) ggCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGaa < 1:611243/100‑1 (MQ=255) ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc > 2:17983/1‑100 (MQ=255) ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc > 2:800653/1‑100 (MQ=255) tgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa > 2:1953184/1‑100 (MQ=255) tgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa > 1:1508857/1‑100 (MQ=255) gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgagatcggaaga > 2:1590122/1‑92 (MQ=255) gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa < 1:1149291/99‑1 (MQ=255) gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAc > 1:1249894/1‑100 (MQ=255) nctgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAcat > 2:2113677/2‑98 (MQ=255) ctgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGACGCCGAGTGGGAAACGCAACCTg > 1:2121040/1‑100 (MQ=255) tgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTgc < 1:1636655/100‑1 (MQ=255) tgtctggTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAATCAGACTGGCAGCTATTGAAGCCGAGTGGGAAACGCAACCTg > 2:1681393/1‑99 (MQ=255) gtctgtTATTGTTCTGGGTGATGAATACGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGATGCCGAGTGGGAAACGCAACCTgcg < 2:1068823/100‑1 (MQ=255) tgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct > 1:1697273/1‑100 (MQ=255) gtTCTTGTTCTGGGTGATGAATCCGGCTACGATATGGGCGGCGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctg < 2:1213807/100‑1 (MQ=255) gtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct > 1:635942/1‑99 (MQ=255) gtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct > 1:2128223/1‑99 (MQ=255) tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTCTTGAAGCCGAGTGGGAAACGCAACCTGCGCctg > 2:2395249/1‑98 (MQ=255) aTTGTTCTGGGTGTTGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgctg > 2:1480876/1‑100 (MQ=255) tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc > 1:1587291/1‑100 (MQ=255) tCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCttt < 1:1363476/100‑1 (MQ=255) cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa < 1:1976841/100‑1 (MQ=255) tGGGTGATGAATCCGGCTGCGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc > 1:2190956/1‑100 (MQ=255) tGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc < 2:1714693/100‑1 (MQ=255) tGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc > 1:1134836/1‑100 (MQ=255) gTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTActct > 1:1989247/1‑100 (MQ=255) gatgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGt > 1:1774102/1‑100 (MQ=255) atgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGt < 1:17984/99‑1 (MQ=255) tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc > 1:1366909/1‑100 (MQ=255) tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc > 1:1576211/1‑100 (MQ=255) gaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCg > 2:841526/1‑100 (MQ=255) gaAGCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc < 2:2026709/99‑1 (MQ=255) aaTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTTCGCCTGCTGCCTTTACTCTGTTCg > 2:212395/1‑99 (MQ=255) aTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc < 1:2289444/100‑1 (MQ=255) ccGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAt < 1:2047863/100‑1 (MQ=255) gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc < 1:1481591/100‑1 (MQ=255) gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc < 1:1701854/100‑1 (MQ=255) cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt > 2:851104/1‑100 (MQ=255) cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt < 2:1362698/100‑1 (MQ=255) tACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTg > 1:1970945/1‑100 (MQ=255) gAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATc < 1:2258499/100‑1 (MQ=255) ggCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAag < 2:2310045/100‑1 (MQ=255) gCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAaga > 2:1972072/1‑100 (MQ=255) cGACGTGCAGAAAAACAAACTGGCTGCTATTTAAGCCGAGTGGGAATCGCAACCTGCGCCTGCTGCCCTTACTCTGTTCTGCATTCCTGATCAGGAagag < 2:1494443/100‑1 (MQ=255) cGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAagag < 2:38387/100‑1 (MQ=255) gACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgagg < 1:884099/100‑1 (MQ=255) gTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGgaga < 1:600274/100‑1 (MQ=255) gTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACGCTGTTCGGCATTCCTGATCAGGAAGAGgaga < 1:1611050/100‑1 (MQ=255) tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc < 2:2296031/100‑1 (MQ=255) tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc < 2:1823506/100‑1 (MQ=255) tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc > 1:1889427/1‑100 (MQ=255) gCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACg < 1:959023/100‑1 (MQ=255) gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTNTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc > 2:945670/1‑100 (MQ=255) gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc < 1:1977116/100‑1 (MQ=255) aaaaACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACa < 2:1573932/100‑1 (MQ=255) | CTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACA > NC_000913/772119‑772304 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |