Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 772,209 C→A 100% T251N (ACC→AAC)  cydA → cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913772,2090CA97.1% 220.0 / ‑0.8 70T251N (ACC→AAC) cydAcytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (30/37);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

CTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACA  >  NC_000913/772119‑772304
                                                                                          |                                                                                               
cTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgc                                                                                        <  1:1252651/100‑1 (MQ=255)
cTATCGCTGCCAACTTCGGTATGGCGGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgc                                                                                        <  2:850450/100‑1 (MQ=255)
 tATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgct                                                                                       >  2:554097/1‑100 (MQ=255)
    cGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGgctgct                                                                                    <  2:125624/100‑1 (MQ=255)
       tGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAt                                                                                  >  1:1465971/1‑99 (MQ=255)
        gCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTg                                                                                >  1:1252023/1‑100 (MQ=255)
         ccAGCTTCGGTATGGCTTCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGa                                                                               <  1:2394423/100‑1 (MQ=255)
         ccAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGa                                                                               <  1:981322/100‑1 (MQ=255)
           aGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAg                                                                             <  1:77039/100‑1 (MQ=255)
           aGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAg                                                                             <  2:1120212/100‑1 (MQ=255)
           aGCTTCGGTATGGCTGCTGTGCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAtt                                                                                 <  2:2381121/96‑1 (MQ=255)
              ttCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCg                                                                          <  1:2355138/100‑1 (MQ=255)
                 ggTATGGCTGCTGTTATGTCTGTTATAGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGt                                                                       >  2:788318/1‑100 (MQ=255)
                  tcttccgatctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTg                                                                      <  1:1590142/91‑1 (MQ=255)
                   tATGGCTGCTGTTCTGTCGGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg                                                                     <  2:2310687/100‑1 (MQ=255)
                      ggCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGaa                                                                  <  1:611243/100‑1 (MQ=255)
                        ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc                                                                >  2:17983/1‑100 (MQ=255)
                        ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc                                                                >  2:800653/1‑100 (MQ=255)
                            tgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa                                                            >  2:1953184/1‑100 (MQ=255)
                            tgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa                                                            >  1:1508857/1‑100 (MQ=255)
                             gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgagatcggaaga                                                           >  2:1590122/1‑92 (MQ=255)
                             gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCaa                                                            <  1:1149291/99‑1 (MQ=255)
                             gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAc                                                           >  1:1249894/1‑100 (MQ=255)
                               nctgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAcat                                                         >  2:2113677/2‑98 (MQ=255)
                                ctgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGACGCCGAGTGGGAAACGCAACCTg                                                        >  1:2121040/1‑100 (MQ=255)
                                 tgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTgc                                                       <  1:1636655/100‑1 (MQ=255)
                                 tgtctggTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAATCAGACTGGCAGCTATTGAAGCCGAGTGGGAAACGCAACCTg                                                        >  2:1681393/1‑99 (MQ=255)
                                  gtctgtTATTGTTCTGGGTGATGAATACGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGATGCCGAGTGGGAAACGCAACCTgcg                                                      <  2:1068823/100‑1 (MQ=255)
                                     tgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct                                                   >  1:1697273/1‑100 (MQ=255)
                                      gtTCTTGTTCTGGGTGATGAATCCGGCTACGATATGGGCGGCGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctg                                                  <  2:1213807/100‑1 (MQ=255)
                                      gtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct                                                   >  1:635942/1‑99 (MQ=255)
                                      gtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct                                                   >  1:2128223/1‑99 (MQ=255)
                                        tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTCTTGAAGCCGAGTGGGAAACGCAACCTGCGCctg                                                  >  2:2395249/1‑98 (MQ=255)
                                         aTTGTTCTGGGTGTTGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgctg                                               >  2:1480876/1‑100 (MQ=255)
                                           tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc                                             >  1:1587291/1‑100 (MQ=255)
                                              tCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCttt                                          <  1:1363476/100‑1 (MQ=255)
                                               cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa                                         <  1:1976841/100‑1 (MQ=255)
                                                tGGGTGATGAATCCGGCTGCGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc                                        >  1:2190956/1‑100 (MQ=255)
                                                tGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc                                        <  2:1714693/100‑1 (MQ=255)
                                                tGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc                                        >  1:1134836/1‑100 (MQ=255)
                                                   gTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTActct                                     >  1:1989247/1‑100 (MQ=255)
                                                     gatgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGt                                   >  1:1774102/1‑100 (MQ=255)
                                                      atgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGt                                   <  1:17984/99‑1 (MQ=255)
                                                       tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc                                 >  1:1366909/1‑100 (MQ=255)
                                                       tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc                                 >  1:1576211/1‑100 (MQ=255)
                                                        gaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCg                                >  2:841526/1‑100 (MQ=255)
                                                        gaAGCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc                                 <  2:2026709/99‑1 (MQ=255)
                                                         aaTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTTCGCCTGCTGCCTTTACTCTGTTCg                                >  2:212395/1‑99 (MQ=255)
                                                          aTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc                              <  1:2289444/100‑1 (MQ=255)
                                                            ccGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAt                            <  1:2047863/100‑1 (MQ=255)
                                                               gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc                         <  1:1481591/100‑1 (MQ=255)
                                                               gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc                         <  1:1701854/100‑1 (MQ=255)
                                                                cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt                        >  2:851104/1‑100 (MQ=255)
                                                                cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt                        <  2:1362698/100‑1 (MQ=255)
                                                                 tACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTg                       >  1:1970945/1‑100 (MQ=255)
                                                                    gAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATc                    <  1:2258499/100‑1 (MQ=255)
                                                                          ggCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAag              <  2:2310045/100‑1 (MQ=255)
                                                                           gCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAaga             >  2:1972072/1‑100 (MQ=255)
                                                                            cGACGTGCAGAAAAACAAACTGGCTGCTATTTAAGCCGAGTGGGAATCGCAACCTGCGCCTGCTGCCCTTACTCTGTTCTGCATTCCTGATCAGGAagag            <  2:1494443/100‑1 (MQ=255)
                                                                            cGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAagag            <  2:38387/100‑1 (MQ=255)
                                                                             gACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgagg           <  1:884099/100‑1 (MQ=255)
                                                                                gTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGgaga        <  1:600274/100‑1 (MQ=255)
                                                                                gTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACGCTGTTCGGCATTCCTGATCAGGAAGAGgaga        <  1:1611050/100‑1 (MQ=255)
                                                                                 tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc       <  2:2296031/100‑1 (MQ=255)
                                                                                 tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc       <  2:1823506/100‑1 (MQ=255)
                                                                                 tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc       >  1:1889427/1‑100 (MQ=255)
                                                                                  gCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACg      <  1:959023/100‑1 (MQ=255)
                                                                                     gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTNTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc   >  2:945670/1‑100 (MQ=255)
                                                                                     gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc   <  1:1977116/100‑1 (MQ=255)
                                                                                      aaaaACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACa  <  2:1573932/100‑1 (MQ=255)
                                                                                          |                                                                                               
CTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACA  >  NC_000913/772119‑772304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: