Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,100,233 G→A 100% intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,2330GA98.8% 221.7 / ‑5.7 69intergenic (+39/‑63)ycdY/ycdZredox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (29/38);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCA  >  NC_000913/1100137‑1100323
                                                                                                |                                                                                          
aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAATAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                         <  1:2226854/100‑1 (MQ=255)
aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                         <  1:1790364/100‑1 (MQ=255)
 aCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgt                                                                                        >  2:2317195/1‑100 (MQ=255)
   ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                      <  1:7285/100‑1 (MQ=255)
   ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                      <  1:1742005/100‑1 (MQ=255)
   ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                      <  2:1515690/100‑1 (MQ=255)
    cgcgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTTATCTACGTCTCTCATAACTGCAACGGATAATTTATTGTTGc                                                                                     <  2:657535/100‑1 (MQ=255)
    cgcgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGc                                                                                     <  2:1354105/100‑1 (MQ=255)
        aTGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaa                                                                                 >  2:1863506/1‑100 (MQ=255)
         tGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaa                                                                                >  2:1109963/1‑100 (MQ=255)
          gCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaaa                                                                               <  2:1082552/100‑1 (MQ=255)
          gCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaaa                                                                               <  1:579242/100‑1 (MQ=255)
            cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                             <  2:2210539/100‑1 (MQ=255)
            cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                             >  2:1729109/1‑100 (MQ=255)
            cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                             >  1:1060331/1‑100 (MQ=255)
             aTTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgt                                                                            >  1:2184300/1‑100 (MQ=255)
              ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg                                                                           >  2:494236/1‑100 (MQ=255)
               tattgcAATGTGGGACGCGCTGGAGGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgt                                                                          <  2:2285959‑M2/94‑1 (MQ=255)
                aGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                         >  1:2202765/1‑100 (MQ=255)
                aGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                         <  2:147487/100‑1 (MQ=255)
                 gtgcAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                         <  1:993044‑M2/95‑1 (MQ=255)
                 gtgcAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                        >  2:826239‑M2/5‑100 (MQ=255)
                   gcAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc                                                                      <  1:1666030‑M2/98‑1 (MQ=255)
                       tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc                                                                  <  2:657507/100‑1 (MQ=255)
                          gggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtca                                                               >  2:1285344/1‑100 (MQ=255)
                            gACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATACTTTATTGTTGCATAAAATGTGTGCTCGATCtcatt                                                             >  1:448895/1‑100 (MQ=255)
                             aCGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattc                                                            <  1:2300626/100‑1 (MQ=255)
                               gAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattca                                                           <  1:782028/99‑1 (MQ=255)
                                 gCTGGAAGAAGATTCTGAAGAAGAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATgg                                                        >  2:1387005/1‑100 (MQ=255)
                                  cTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGc                                                       <  2:1639405/100‑1 (MQ=255)
                                  cTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGc                                                       <  2:1605837/100‑1 (MQ=255)
                                   tGGAATAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTAGCATAAAATGTGTGCTCGATCTCATTCATgg                                                        >  2:859548/1‑98 (MQ=255)
                                    ngaagaaGATTCTGAAGAATAAGTGTGATCTACGACACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGCTCTCATTCATGGCcg                                                     >  2:996886/2‑100 (MQ=255)
                                    ggAAGATGATTCTGAAGAATAAGTGTGTTCTACGTTAATCATAACTGCAACGGATAATTTATTGTTGCATTAAATGTGTGCTCGATCGCATGAATGGCcg                                                     <  2:951983/100‑1 (MQ=255)
                                     gaagaaGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATACTGTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcgc                                                    >  1:1413532/1‑100 (MQ=255)
                                     gaagaaGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcgc                                                    >  1:1567243/1‑100 (MQ=255)
                                     gaagaaGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcgc                                                    >  2:1562453/1‑100 (MQ=255)
                                      aagaagATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAGCTCATTCATGGCcgcg                                                   >  2:2178820/1‑100 (MQ=255)
                                         aagaTTCTGCGGAATAAGTGTGCTCTACGTAACTCACAACTGCAACGGCTTATTTGTTGTTGCATATAATGCGTTCGCGATCTAATTCATGGGcgcggt                                                 <  2:1034672/99‑3 (MQ=255)
                                         aagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGttt                                                <  1:1386228/100‑1 (MQ=255)
                                          agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                               >  1:2259653/1‑100 (MQ=255)
                                               cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCt                                             >  1:2166474/1‑97 (MQ=255)
                                               cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt                                          <  2:1056018/100‑1 (MQ=255)
                                                tGAAGAATAAGTGTGATCTACATCACTCATAACTGAAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATATCATTCATGGCCGCGTTTTATGc                                           >  2:648613/1‑98 (MQ=255)
                                                 gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                        >  2:1407917/1‑100 (MQ=255)
                                                 gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                        <  2:1676244/100‑1 (MQ=255)
                                                   agaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATTTCATTCATGGCCGCGTTTTCTGCTATc                                       <  2:2233835/99‑1 (MQ=255)
                                                   agaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCa                                      <  1:1455438/100‑1 (MQ=255)
                                                        aaGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcgc                                 <  1:1308031/100‑1 (MQ=255)
                                                         aGTGTGATCTACGTCACTCATAACTGAAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcgc                                 >  2:1343319/1‑99 (MQ=255)
                                                          ntgtgATCTACGTCACTCATAACTGCAACGGATAATTTAATGTTGCATAAAAAGAGTGTTCGATCACATTCATGGCAGCGTTTTCTGCTATCATgc                                   >  2:881608/2‑96 (MQ=255)
                                                          gtatgATCAACGTCAAACATAACAGCAACGGATAATATATTGTTACCCAAAATGTGTGCTCGATCTCATTAATAGTAGAGATTTATGCTATCATgcac                                 >  2:1255296/4‑96 (MQ=255)
                                                           tgtgATCTACGTCACTCATAACTGCAACGTATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGc                              <  2:228662/100‑1 (MQ=255)
                                                            gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                             <  1:1879292/100‑1 (MQ=255)
                                                            gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                             <  1:1108570/100‑1 (MQ=255)
                                                             tgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCAt                            >  2:786170/1‑100 (MQ=255)
                                                              gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                           >  2:434908/1‑100 (MQ=255)
                                                               aTCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                           <  2:627405/99‑1 (MQ=255)
                                                                tCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa                         <  2:1998237/100‑1 (MQ=255)
                                                                  tACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACa                       >  2:1320279/1‑100 (MQ=255)
                                                                    cGTCACTCATAACTGCTACGGATAATTTTTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACan                      <  1:996897/99‑2 (MQ=255)
                                                                      tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                   <  1:341899/100‑1 (MQ=255)
                                                                        aCTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtc                 <  1:494239/100‑1 (MQ=255)
                                                                         cTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct                >  2:437527/1‑100 (MQ=255)
                                                                           cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct                >  2:1292174/1‑98 (MQ=255)
                                                                            aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc             <  1:826248/100‑1 (MQ=255)
                                                                            aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc             <  1:2255135/100‑1 (MQ=255)
                                                                               aCTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAt          <  2:132246/100‑1 (MQ=255)
                                                                                 tGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg        <  2:1567224/100‑1 (MQ=255)
                                                                                 tGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg        <  2:443792/100‑1 (MQ=255)
                                                                                  gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc       <  1:1065259/100‑1 (MQ=255)
                                                                                    aaCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCaa     <  1:2079915/100‑1 (MQ=255)
                                                                                      cGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCa      <  2:619065/97‑1 (MQ=255)
                                                                                       tgATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCaagc   <  1:366736/98‑3 (MQ=255)
                                                                                       ggATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATca  <  2:1340728/100‑1 (MQ=255)
                                                                                                |                                                                                          
AACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCA  >  NC_000913/1100137‑1100323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: