| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,129,394 | G→C | 18.6% | intergenic (+20/+20) | murJ → / ← flgN | putative lipid II flippase/export chaperone for FlgK and FlgL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,129,394 | 0 | G | C | 18.6% | 172.6 / 36.8 | 70 | intergenic (+20/+20) | murJ/flgN | putative lipid II flippase/export chaperone for FlgK and FlgL |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base C (6/7); total (34/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01 | |||||||||||
GGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGC > NC_000913/1129296‑1129492 | ggCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt < 2:744205/100‑1 (MQ=255)ggCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt > 2:2396545/1‑100 (MQ=255) cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc < 1:1181349/100‑1 (MQ=255) cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc < 1:975444/100‑1 (MQ=255) cGGGAATTGCCGCGTACTTCGCTGCTCTGGCGGTACTGGGCTTCACAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGTATTCCGGCCtgcagtgc < 2:1270819/100‑1 (MQ=255) gtgATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag < 1:1322106/98‑1 (MQ=255) gATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGc < 2:366257/100‑1 (MQ=255) ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCg < 1:319880/100‑1 (MQ=255) ttccgatctcTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg < 1:1765111/91‑1 (MQ=255) tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg > 2:1383241/1‑100 (MQ=255) tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg < 1:1731231/100‑1 (MQ=255) tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg < 2:1377879/100‑1 (MQ=255) gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGga < 1:983295/100‑1 (MQ=255) gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGga < 1:1285106/100‑1 (MQ=255) gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGga < 1:514205/100‑1 (MQ=255) ccGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgaa > 1:1636988/1‑99 (MQ=255) gcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGAt > 2:242155/1‑100 (MQ=255) gcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaatg < 1:742787/100‑4 (MQ=255) tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGga < 1:1408606/94‑1 (MQ=255) tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAAt > 2:1207920/1‑100 (MQ=255) cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGGAGAtcggaa > 2:1765090/1‑95 (MQ=255) cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGACTGCAGTGCAGGTCGGAGATAAt > 2:2234708/1‑98 (MQ=255) cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCAGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCt < 1:1606102/100‑1 (MQ=255) tCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc < 1:2268623/100‑1 (MQ=255) cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa < 2:1693576/100‑1 (MQ=255) cTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGa > 1:2163524/1‑100 (MQ=255) gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTATCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc < 2:1330843/100‑1 (MQ=255) gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc < 2:1788111/100‑1 (MQ=255) cACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCg > 1:736813/1‑100 (MQ=255) ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt < 1:1192088/100‑1 (MQ=255) gCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATc < 2:1077437/100‑1 (MQ=255) tACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtttt < 1:871509/100‑1 (MQ=255) tACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtttt > 2:1803675/1‑100 (MQ=255) tGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAc > 1:600561/1‑100 (MQ=255) gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAGCTTTTTAcc > 2:1630150/1‑100 (MQ=255) cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgcc > 1:1319873/1‑100 (MQ=255) cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgcc > 1:1629092/1‑100 (MQ=255) tCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACcgccgc > 1:1063404/1‑100 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg < 2:253904/100‑1 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg > 1:1735364/1‑100 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg > 1:128306/1‑100 (MQ=255) gTTAAAGAATTTGCCCGCCGGACGATGGAACAATGCATTCCGGCCTGCAGGGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg < 2:169/99‑1 (MQ=255) tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgt < 1:505778/100‑1 (MQ=255) aaaGAATTTGCCCGCCGGACGGAGAAACAATGCATTCCGGCCTGCAGTGCAGGCCGCAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg > 2:1534107/1‑98 (MQ=255) aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttg < 2:1797348/100‑1 (MQ=255) aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttg > 1:2238196/1‑100 (MQ=255) gAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt > 1:902416/1‑100 (MQ=255) ttGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAgg < 2:1248452/100‑1 (MQ=255) tcctgccaGACGGTGTAACAATTCATTCCGGCCTACAGTGCAGGCCGGAGAGAATCTTCAGATCGAAATCTTTTTACCTCCGCGATGGATTGTTGAg > 2:946239/5‑97 (MQ=255) gCCCGCCGGACGGTGTAACAATGCATTCCTGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTc < 2:2034349/100‑1 (MQ=255) cgccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGa > 1:1887994/1‑100 (MQ=255) cgccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGa > 1:2104662/1‑100 (MQ=255) gccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAc < 1:90995/100‑1 (MQ=255) gccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAc < 2:736805/100‑1 (MQ=255) ccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc < 1:593184/100‑1 (MQ=255) cgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCg < 1:1399155/100‑1 (MQ=255) ggATGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCGTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTGGAGGTCTGACCg < 2:526085/99‑1 (MQ=255) ggACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc < 1:722169/98‑1 (MQ=255) cGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCg < 1:1639484/100‑1 (MQ=255) gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc > 2:1249926/1‑100 (MQ=255) gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc > 2:2298343/1‑100 (MQ=255) tAACAGTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa < 1:867060/100‑1 (MQ=255) tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa < 2:1242351/100‑1 (MQ=255) tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa < 1:2350424/100‑1 (MQ=255) tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTAAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACTGTTCGCCCCa > 2:1932153/1‑100 (MQ=255) aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAg > 1:2383702/1‑100 (MQ=255) cATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGt > 1:1280237/1‑100 (MQ=255) tctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc > 1:1693852/3‑100 (MQ=255) tctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAAATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGt > 1:1693866/3‑99 (MQ=255) ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCg > 1:963161/1‑100 (MQ=255) tCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCgg > 2:809855/1‑100 (MQ=255) tCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCg > 1:2102532/1‑99 (MQ=255) gCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTcc > 1:41236/1‑100 (MQ=255) ccTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt < 1:1719648/100‑1 (MQ=255) cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt < 1:1508735/99‑1 (MQ=255) cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg > 1:539699/1‑100 (MQ=255) gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGAt > 2:917408/1‑100 (MQ=255) gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGAt > 1:1804740/1‑100 (MQ=255) agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGc < 1:620066/100‑1 (MQ=255) agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGc < 1:1960984/100‑1 (MQ=255) | GGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGC > NC_000913/1129296‑1129492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |