Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,143,125 C→T 100% D415N (GAC→AAC)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,1250CT98.2% 167.1 / ‑5.3 53D415N (GAC→AAC) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (28/24);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAG  >  NC_000913/1143029‑1143218
                                                                                                |                                                                                             
ttGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacg                                                                                            <  2:2256487/100‑1 (MQ=255)
  ggCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgca                                                                                          >  2:294975/1‑100 (MQ=255)
   gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcac                                                                                         >  2:1406911/1‑100 (MQ=255)
   gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcac                                                                                         >  1:2095987/1‑100 (MQ=255)
        ggAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGc                                                                                    >  2:678147/1‑100 (MQ=255)
          aaCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCa                                                                                  >  1:515507/1‑100 (MQ=255)
           aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAg                                                                                 >  2:335217/1‑100 (MQ=255)
            cgaTGGCGTGAACATCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGt                                                                                >  1:1625761/3‑100 (MQ=255)
            cAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGt                                                                                >  2:1640967/1‑100 (MQ=255)
                 gCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAg                                                                           <  2:573475/100‑1 (MQ=255)
                  cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGa                                                                          >  1:2177716/1‑100 (MQ=255)
                    tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaac                                                                        <  2:1015578/100‑1 (MQ=255)
                    tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaac                                                                        >  1:1310372/1‑100 (MQ=255)
                     gAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaaca                                                                       >  2:930046/1‑100 (MQ=255)
                           ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg                                                                 <  1:1331523/100‑1 (MQ=255)
                                 tgtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAAc                                                           <  1:1305963/100‑1 (MQ=255)
                                  gtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                          <  2:288991/100‑1 (MQ=255)
                                  gtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                          <  2:195753/100‑1 (MQ=255)
                                  gtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                          >  2:1453296/1‑100 (MQ=255)
                                    tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg                                                        <  2:411403/100‑1 (MQ=255)
                                    tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg                                                        <  1:2049306/100‑1 (MQ=255)
                                    tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGt                                                         >  1:330963/1‑99 (MQ=255)
                                     ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                       <  1:294978/100‑1 (MQ=255)
                                           cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg                                                 >  2:1740719/1‑100 (MQ=255)
                                           cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg                                                 <  1:1555161/100‑1 (MQ=255)
                                            aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg                                                <  1:909762/100‑1 (MQ=255)
                                               gcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAtt                                             <  2:1705454/100‑1 (MQ=255)
                                                cttcttcttCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTc                                            <  2:1497264/100‑1 (MQ=255)
                                                 ttcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCa                                           <  1:1211779/100‑1 (MQ=255)
                                                 ttcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCa                                           <  1:1616095/100‑1 (MQ=255)
                                                       ttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGACCAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                     >  2:1153827/1‑100 (MQ=255)
                                                       ttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                     <  2:228208/100‑1 (MQ=255)
                                                       ttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                     <  2:515504/100‑1 (MQ=255)
                                                        tcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTg                                    <  2:158097/100‑1 (MQ=255)
                                                         cGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGa                                   >  1:842256/1‑100 (MQ=255)
                                                         cGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGa                                   >  2:291184/1‑100 (MQ=255)
                                                          gATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTAACCCAATGa                                   >  1:88135/1‑99 (MQ=255)
                                                          gATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAACAGAACCACGCGAACAAACGTGATGACTGGATTCACCCAGTGAt                                  >  2:1615314/1‑100 (MQ=255)
                                                           atcagttgtaGAATAGTTATCTACAGCTATTCGTTGGTACGCAGGGTGGCAGTATCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                 <  1:1823859/91‑1 (MQ=255)
                                                           aTCAGACTCAGAATAAAAAACGACAGAAATTAGTTGATACGAACGGTGCCAGTAAAAGAACAACGAGGACAAACGTGATGACTTGAATAACCCa                                       >  2:461855/1‑94 (MQ=255)
                                                           aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATTACTGGATTCACCCAGTGATg                                 <  2:2146235/100‑1 (MQ=255)
                                                           aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGAt                                  >  1:613271/1‑99 (MQ=255)
                                                             cAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATggg                               >  1:2076156/1‑100 (MQ=255)
                                                               gACGCGGACTAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCTAGTACCAGAACAACGCGGACAATCGTGATGACTGGACTCACCGAGTGATGGGc                              >  2:988572/1‑99 (MQ=255)
                                                                 cGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGATGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCa                           >  2:1041033/1‑100 (MQ=255)
                                                                       aTAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCt                     <  2:741786/100‑1 (MQ=255)
                                                                          gagagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc                  >  1:56372/1‑100 (MQ=255)
                                                                           agagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACg                 >  2:1445734/1‑100 (MQ=255)
                                                                                gACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACa            <  1:1445749/100‑1 (MQ=255)
                                                                                  cAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt          >  1:1793515/1‑100 (MQ=255)
                                                                                  cAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt          >  1:478217/1‑100 (MQ=255)
                                                                                   aGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAttt         <  1:357453/100‑1 (MQ=255)
                                                                                         tCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagca   >  1:2258122/1‑100 (MQ=255)
                                                                                          cGTTGTTACGCACGGTGCCAGTACCATAACAACGCGGACAAACGGGATGACTGGATTAACCCAGTGATGTGCTCAGGCGCTTATGGGACATGTCcagcag  <  2:1142157/100‑1 (MQ=255)
                                                                                          cGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag  >  1:2262332/1‑100 (MQ=255)
                                                                                          cGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag  >  1:1069707/1‑100 (MQ=255)
                                                                                          cGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag  <  1:1409321/100‑1 (MQ=255)
                                                                                                |                                                                                             
TTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAG  >  NC_000913/1143029‑1143218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: