Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,219,033 C→T 35.8% S17S (TCC→TCT ymgF → inner membrane division septum protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,219,0330CT35.8% 106.4 / 97.6 106S17S (TCC→TCTymgFinner membrane division septum protein
Reads supporting (aligned to +/- strand):  ref base C (31/37);  new base T (25/13);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 6.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

Only 100 of 108 total aligned reads displayed.

GTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATGCCCTT  >  NC_000913/1218936‑1219132
                                                                                                 |                                                                                                   
gtttgCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCAt                                                                                                   <  2:1658637/100‑1 (MQ=255)
  ttgCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTg                                                                                                 >  2:301832/1‑100 (MQ=255)
    gCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCa                                                                                               >  2:1061877/1‑100 (MQ=255)
     cTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCAt                                                                                              <  1:1279744/100‑1 (MQ=255)
      tataATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCAtt                                                                                             >  2:2372128/1‑100 (MQ=255)
      tataATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCAtt                                                                                             <  1:399363/100‑1 (MQ=255)
          aTTGTTTGAAAGTTTTGACAGGATTGCCATTGGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTAt                                                                                          >  2:1721193/1‑99 (MQ=255)
           ttGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGc                                                                                        <  2:2344879/100‑1 (MQ=255)
           ttGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGc                                                                                        <  2:541063/100‑1 (MQ=255)
            tGTTTGAAAGTTTTGACAGGATTTCTTTTAGTAGTATGAACAATAGTAATAGTCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCt                                                                                       <  1:819460/100‑1 (MQ=255)
            tGTTTGAAAGTTTTGACAGGATTGCCATTCGTAGCATGAACAATAGTAATAATCTGGATTATTTCATTCTCTATATCATATTTTCCATTGCATTTATGCt                                                                                       <  2:1438948/100‑1 (MQ=255)
            tGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCt                                                                                       <  2:670330/100‑1 (MQ=255)
             gTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTg                                                                                      >  1:408799/1‑100 (MQ=255)
             gTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTg                                                                                      <  2:1058322/100‑1 (MQ=255)
             gTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCt                                                                                       >  1:147873/1‑99 (MQ=255)
               ttGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGAt                                                                                    >  1:540872/1‑100 (MQ=255)
               ttGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGAt                                                                                    <  2:692790/100‑1 (MQ=255)
               ttGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGAt                                                                                    >  1:2385188/1‑100 (MQ=255)
               ttGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGAt                                                                                    >  1:2373254/1‑100 (MQ=255)
                tGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATc                                                                                   >  1:718933/1‑100 (MQ=255)
                tGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATc                                                                                   >  1:2025878/1‑100 (MQ=255)
                 gAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCa                                                                                  >  2:1564684/1‑100 (MQ=255)
                 gAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCa                                                                                  >  1:320871/1‑100 (MQ=255)
                 gAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTAtaataaaca                                                                                  >  1:106132/1‑92 (MQ=255)
                  aaaGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCa                                                                                  <  2:315927/99‑1 (MQ=255)
                     gTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACcct                                                                              <  2:1112874/100‑1 (MQ=255)
                     gTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACcct                                                                              <  2:1113004/100‑1 (MQ=255)
                       tttGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACcctcc                                                                            <  1:344714/100‑1 (MQ=255)
                        ttGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACcctcct                                                                           <  1:2104893/100‑1 (MQ=255)
                         tGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTg                                                                          >  1:1981171/1‑100 (MQ=255)
                         tGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTg                                                                          >  2:289828/1‑100 (MQ=255)
                         tGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTg                                                                          <  2:1876110/100‑1 (MQ=255)
                         tGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATACTGATCACCATCCTg                                                                          >  1:1944073/1‑100 (MQ=255)
                          gACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTgg                                                                         <  2:173408/100‑1 (MQ=255)
                          gACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTgg                                                                         >  1:1572701/1‑100 (MQ=255)
                          gACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTgg                                                                         <  2:1834343/100‑1 (MQ=255)
                          gACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTgg                                                                         >  1:1748626/1‑100 (MQ=255)
                           aCAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGt                                                                        <  2:616471/100‑1 (MQ=255)
                           aCAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGt                                                                        >  2:1566548/1‑100 (MQ=255)
                           aCAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGt                                                                        <  2:398979/100‑1 (MQ=255)
                            cAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTc                                                                       >  2:566265/1‑100 (MQ=255)
                            cAGGATTGCAATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTc                                                                       <  2:2385160/100‑1 (MQ=255)
                                   gCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTa                                                                >  2:1823511/1‑100 (MQ=255)
                                     cATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTAtt                                                              >  2:1461141/1‑100 (MQ=255)
                                     cATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTAtt                                                              >  2:1458475/1‑100 (MQ=255)
                                     cATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTAt                                                               <  2:1185799/99‑1 (MQ=255)
                                      aTTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTg                                                             <  2:1608640/100‑1 (MQ=255)
                                         agtagCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCaa                                                          <  2:1227742/100‑1 (MQ=255)
                                             gCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAAcc                                                      >  2:2267354/1‑100 (MQ=255)
                                             gCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAAcc                                                      >  2:126357/1‑100 (MQ=255)
                                              cATGAACAATAGTAATAATCTGGATTATTTAACACTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTACTGCAAAAccc                                                     >  2:1576455/1‑100 (MQ=255)
                                                tGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAg                                                   >  2:146993/1‑100 (MQ=255)
                                                tGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAg                                                   <  1:2020133/100‑1 (MQ=255)
                                                 gAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCGCCCTCCTGGTCATCCTTATTTCAAAAccaa                                                    >  1:1978034/1‑96 (MQ=255)
                                                 gAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTTATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGt                                                  >  1:1700867/1‑100 (MQ=255)
                                                 gAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGt                                                  >  2:2358277/1‑100 (MQ=255)
                                                 gAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGt                                                  >  1:1211001/1‑100 (MQ=255)
                                                  aaCAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGt                                                  <  1:2212844/99‑1 (MQ=255)
                                                  aaCAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGt                                                  <  2:1604746/99‑1 (MQ=255)
                                                    cAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTAcc                                               >  2:673152/1‑100 (MQ=255)
                                                     aaTAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCg                                              <  1:1061887/100‑1 (MQ=255)
                                                      aTAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCgg                                             <  1:30497/100‑1 (MQ=255)
                                                      aTAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCgg                                             <  2:1785759/100‑1 (MQ=255)
                                                        aGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGc                                           >  1:613025/1‑100 (MQ=255)
                                                         gTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTTGTCATCCTTATTGCAAAACCCAGTAc                                                >  2:1068496/1‑94 (MQ=255)
                                                          taataaTCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTg                                         >  1:1932172/1‑100 (MQ=255)
                                                          taataaTCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTg                                         <  2:373715/100‑1 (MQ=255)
                                                            ataatCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTggg                                       <  2:490468/100‑1 (MQ=255)
                                                             taatCTGGATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGga                                      >  1:2088969/1‑100 (MQ=255)
                                                                tCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGaa                                   >  1:655518/1‑100 (MQ=255)
                                                                 cTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAg                                  >  2:616635/1‑100 (MQ=255)
                                                                   ggATTATTTCACTCTCTATATCATATTTTCCATTGCTTTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTg                                >  1:1688544/1‑100 (MQ=255)
                                                                   ggATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTTATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGt                                 >  2:207428/1‑99 (MQ=255)
                                                                   ggATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTg                                <  2:201848/100‑1 (MQ=255)
                                                                    gATTATTTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGc                               >  2:1298722/1‑100 (MQ=255)
                                                                     attattTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCt                              <  1:918359/100‑1 (MQ=255)
                                                                     attattTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCt                              >  2:335554/1‑100 (MQ=255)
                                                                      ttattTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCtt                             >  1:1956157/1‑100 (MQ=255)
                                                                       tattTCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACTCTCCTGGTCATCCTTATTGCAAAACTCAGTACCGGGCTGGGAGAAGTGCTtg                            >  2:1134449/1‑100 (MQ=255)
                                                                       tattTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTtg                            <  2:1517756/100‑1 (MQ=255)
                                                                        attTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTtgt                           <  2:2137647/100‑1 (MQ=255)
                                                                         tttCACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTtgtg                          <  2:540869/100‑1 (MQ=255)
                                                                          ttCACGCTCTACATCATATTTTCCATTGCATGTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGATGGGAGAAGTGCTtgtg                          <  2:439144/99‑1 (MQ=255)
                                                                           tCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGAc                        >  2:1125940/1‑100 (MQ=255)
                                                                            cACTCTCTATATCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACg                       <  1:1231480/100‑1 (MQ=255)
                                                                                ctctATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGTTCATCCTTATTGCAAAACCTAGTACAGAGATGGGAGAAGTTCTTGTGACGATaa                   >  2:846562/1‑100 (MQ=255)
                                                                                  ctATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAAt                 <  2:282780/100‑1 (MQ=255)
                                                                                   tataTCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAAtt                <  2:1981149/100‑1 (MQ=255)
                                                                                   tataTCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAAtt                <  2:1630370/100‑1 (MQ=255)
                                                                                     tatCATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTg              >  1:2126739/1‑100 (MQ=255)
                                                                                       tCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGTAGAAGTGCTTGTGACGATAAATTTGc             <  2:561404/99‑1 (MQ=255)
                                                                                       tCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCt            >  2:1792029/1‑100 (MQ=255)
                                                                                        cATATTTTCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGCTAAATTTGCtt           >  1:1180886/1‑100 (MQ=255)
                                                                                           atTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAAt        <  1:1902639/100‑1 (MQ=255)
                                                                                            ttttCTATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATg       >  2:1232518/1‑100 (MQ=255)
                                                                                            ttttCCATTGTATTTATGCTGATCAGCCTCCTTGTCATCCTTATTGCAAAACCCATTACCGGTCTGGGAGAAGTGCTTGTGACTATAAATTTGCtt           <  2:385229/96‑1 (MQ=255)
                                                                                            ttttCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGTTTAATg       >  1:1545529/1‑100 (MQ=255)
                                                                                              ttCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATGcc     <  1:1085598/100‑1 (MQ=255)
                                                                                                ccATTGCATTTATGCTGATCACCCGCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATGCCCt   <  2:1944051/100‑1 (MQ=255)
                                                                                                 cATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATGCCCtt  <  1:731232/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTGGATTATTTCACTCTCTATATCATATTTTCCATTGCATTTATGCTGATCACCCTCCTGGTCATCCTTATTGCAAAACCCAGTACCGGGCTGGGAGAAGTGCTTGTGACGATAAATTTGCTTAATGCCCTT  >  NC_000913/1218936‑1219132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: