Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,944 T→C 100% intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,9440TC97.4% 248.3 / ‑2.4 77intergenic (‑22/‑583)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (40/34);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTG  >  NC_000913/1292848‑1293036
                                                                                                |                                                                                            
cGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgg                                                                                           <  1:2305837/100‑1 (MQ=255)
 gTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTggg                                                                                          >  1:291704/1‑100 (MQ=255)
  tttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                         <  2:291701/100‑1 (MQ=255)
  tttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                         <  2:40315/100‑1 (MQ=255)
    tCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtg                                                                                       <  1:835260/100‑1 (MQ=255)
         tgtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttg                                                                                  <  2:748393/100‑1 (MQ=255)
            aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCATAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgttg                                                                               <  2:658667/100‑1 (MQ=255)
            aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgttg                                                                               <  2:869757/100‑1 (MQ=255)
            aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgttg                                                                               <  1:1876450/100‑1 (MQ=255)
            aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCACTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTttgttg                                                                               >  1:1510622/1‑100 (MQ=255)
             cATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGa                                                                              >  2:1205563/1‑100 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                            <  2:2381155/100‑1 (MQ=255)
                tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGt                                                                           <  2:1569207/100‑1 (MQ=255)
                tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTGAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGt                                                                           <  1:1449530/100‑1 (MQ=255)
                 ctctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGta                                                                          >  2:1161491/1‑100 (MQ=255)
                  tctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaa                                                                         >  1:964752/1‑100 (MQ=255)
                    ttGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaata                                                                       <  2:1135635/100‑1 (MQ=255)
                    ttGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaata                                                                       <  1:973862/100‑1 (MQ=255)
                        cTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATag                                                                   >  1:1264532/1‑100 (MQ=255)
                        cTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTAGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATag                                                                   >  2:1450188/1‑100 (MQ=255)
                         tGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATaga                                                                  >  1:2314980/1‑100 (MQ=255)
                          gcgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATagag                                                                 <  2:1641559/100‑1 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                               >  2:729083/1‑99 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                              >  2:174705/1‑100 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                              >  1:982511/1‑100 (MQ=255)
                                 aaGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTaaa                                                          >  2:1970824/1‑100 (MQ=255)
                                 aaGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTaaa                                                          <  2:2065885/100‑1 (MQ=255)
                                  agagTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAAt                                                         >  2:2313857/1‑100 (MQ=255)
                                    agTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTc                                                       <  1:2313885/100‑1 (MQ=255)
                                     gtattgATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGTTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTc                                                       <  2:1068826/94‑1 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                      >  2:428781/1‑100 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                      <  2:1997285/100‑1 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                      <  2:2307355/100‑1 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGt                                                    >  2:1316951/1‑99 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATAGTAGTAATCTCAAACTTATAATGGGATGGTTTGTTTAGGTAATAATAGAGCCTTAAATGAAGtt                                                   >  2:1729933/1‑100 (MQ=255)
                                          gATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgt                                                 >  1:2058646/1‑100 (MQ=255)
                                          gATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgt                                                 >  2:1223662/1‑100 (MQ=255)
                                           aTGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgtg                                                <  1:2210243/100‑1 (MQ=255)
                                           aTGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgtg                                                <  1:1322566/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                               >  2:824025/1‑100 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                               <  2:2321914/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                               >  1:1555485/1‑100 (MQ=255)
                                             gttgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgtg                                                <  1:1236516/98‑1 (MQ=255)
                                               tgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAAt                                            >  2:259459/1‑100 (MQ=255)
                                               tgttCAGAATTTTAAGTGCTTCACTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAAt                                            <  2:1510605/100‑1 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATa                                           >  1:1299563/1‑100 (MQ=255)
                                                  tCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGc                                         >  1:300255/1‑100 (MQ=255)
                                                  tCAGAATTTTAAGTGCTTCGCTCATTATAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAg                                          >  2:2169611/1‑99 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                        >  1:2014688/1‑100 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                       <  2:1299549/100‑1 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACAGGGGAGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                       >  1:1836782/1‑100 (MQ=255)
                                                     ttaTTTTAAGTGCTTCGCACAATGTAATAATCTCAAACTTATACTGGGGTGAATTGTTGAGGTAATAATAGAGACTTAAATTCAGTTGTTCAATAGcaa                                       >  2:421654/3‑97 (MQ=255)
                                                     gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                      >  1:677705/1‑100 (MQ=255)
                                                         tttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAAt                                  >  1:1074350/1‑100 (MQ=255)
                                                         tttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAAt                                  <  2:381374/100‑1 (MQ=255)
                                                          ttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATg                                 >  1:868985/1‑100 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                            <  1:17833/100‑1 (MQ=255)
                                                               tGCTTCGCCCATCGTACGAATCTCAAACTTATACTGAGGCGGTTAGTTGAGGTAATAATATAGAATTAAATTCAGTTGTGCAATAGCCAGGAACGTAAg                             >  2:453384/1‑99 (MQ=255)
                                                                     gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                      >  2:784508/1‑100 (MQ=255)
                                                                     gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                      >  1:1400100/1‑100 (MQ=255)
                                                                       tCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaa                     <  1:869695/99‑1 (MQ=255)
                                                                         aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCAAGGAATGTAAGAAATTCaaaa                   >  1:2004095/1‑99 (MQ=255)
                                                                         aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGACAGGAATGTAAGGAATTCAAAAt                  >  2:1975736/1‑100 (MQ=255)
                                                                            gtagtaATCTCAAACTTATACTGGGGCGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGt               >  2:1294399/1‑100 (MQ=255)
                                                                              agtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTc             <  1:1294413/100‑1 (MQ=255)
                                                                               gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCt            >  2:1248737/1‑100 (MQ=255)
                                                                               gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCt            >  2:130871/1‑100 (MQ=255)
                                                                                 aaTCTCAAACTTATACTGGGGTCGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCttt          <  2:1372134/100‑1 (MQ=255)
                                                                                   tctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAt        >  2:1639679/1‑100 (MQ=255)
                                                                                   tctcAAACTTATACTGGGGAGGTTTCTTGAGGTAAGAATAAAGCCTTAAATTCAGTTGTGCAATAGCGATGAATGTAAGGAATTCAAAATTGTTCTTTAt        >  2:1340170/1‑100 (MQ=255)
                                                                                    ctcAAACTTATCCTTGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt       <  2:1903515/100‑1 (MQ=255)
                                                                                    ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt       >  2:56634/1‑100 (MQ=255)
                                                                                    ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt       >  2:1919745/1‑100 (MQ=255)
                                                                                     tgaaaCTTAGTCTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      <  2:1836761/98‑1 (MQ=255)
                                                                                     tcAAAGTGATACTGAGGTGGTTTGTTGAGGTACTAATAGAGCCATAAATTCAGGTGTTTTCTAGCGAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      <  2:1190603/100‑1 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt     <  2:741742/100‑1 (MQ=255)
                                                                                           ttATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTtgtg  <  1:713714/98‑1 (MQ=255)
                                                                                                |                                                                                            
CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTG  >  NC_000913/1292848‑1293036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑