| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,311,831 | C→T | 16.9% | intergenic (+23/+17) | tonB → / ← yciA | membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,311,831 | 0 | C | T | 16.9% | 169.9 / 24.4 | 78 | intergenic (+23/+17) | tonB/yciA | membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase |
| Reads supporting (aligned to +/- strand): ref base C (33/29); new base T (8/5); total (44/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01 | |||||||||||
AGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATA > NC_000913/1311735‑1311925 | aGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCgg < 2:1892093/100‑1 (MQ=255) gCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGa < 2:837258/100‑1 (MQ=255) ccGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAg > 1:938688/1‑100 (MQ=255) ccGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAg < 2:138611/100‑1 (MQ=255) gggTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGc < 2:2103657/100‑1 (MQ=255) tAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCttt < 2:68914/100‑1 (MQ=255) aaGCCAGGCAGTGGGGTTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt < 2:2017928/100‑1 (MQ=255) aaGCCAGGCAGTGGGATTGTGGCGAATATCCTGTTTAAAATTAACGGCACCACCGAAATGCAGTAAGCAGAAAGTCAAAAGCCCCCCACCGGAGGCt > 2:420885/1‑97 (MQ=255) aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGTCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg < 1:2320444/100‑1 (MQ=39) aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg < 1:1130993/100‑1 (MQ=255) aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg < 2:1106761/100‑1 (MQ=255) aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg < 1:2062103/100‑1 (MQ=255) aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg > 1:2227502/1‑100 (MQ=255) gCCAGGCAGTGGGAGTGTGGTGAATATCCTGTTTAAAATTAACGGCACCAACGAAATTCAGTAAGTAGAAAGTCAAAAGCCTCCGAACGGAGTCtat > 2:1514759/1‑95 (MQ=255) ccAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGAc < 2:2131972/100‑1 (MQ=255) ccAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc < 1:2070915/100‑1 (MQ=255) ccAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc < 1:2071843/100‑1 (MQ=255) cAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc < 1:799412/99‑1 (MQ=255) ggATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTATTGACTATTACTCaa > 1:2367392/1‑100 (MQ=255) ggagatgAACATCCTCTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACAGGATGATCTTGACCATTCCTAAACa > 2:1247567/6‑98 (MQ=255) ggatatCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTaaaac > 2:921261/3‑97 (MQ=255) atatCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGgc > 1:711391/1‑98 (MQ=255) tCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAg < 1:2392478/100‑1 (MQ=255) ccTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAgg < 1:1339457/100‑1 (MQ=255) gTTTATAATTGACGGGACCACCGAAATTCAGTAAGCAGTAAGTCGTAAGCCGCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGttt < 2:341806/100‑1 (MQ=255) gTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGttt > 1:1531874/1‑100 (MQ=255) gTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGttt > 1:1005699/1‑100 (MQ=255) tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGtttt < 1:851034/100‑1 (MQ=255) tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc > 1:1760435/1‑100 (MQ=255) tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc > 2:611462/1‑100 (MQ=255) tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAATCAAAACCCTCCGAACGGAGGCTTTTGACTATTACTCAACACGTAAGGCGCGAGATTTTcc > 2:962961/1‑100 (MQ=255) aaaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt < 1:950673/100‑1 (MQ=255) aaaTTAACGTTTCCATCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt < 1:803612/99‑1 (MQ=39) aTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGATAAGGCACAAGGTTTTCCTTCa > 1:29249/1‑100 (MQ=255) tAACGGCACCACCGAAATTCAGTAAGCAGAAATTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACACGTAAACCGCGAGGTTTTTCttta > 2:1585784/1‑96 (MQ=255) tAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAgg < 1:724302/100‑1 (MQ=255) cGGCACCACAGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATc > 1:455200/1‑100 (MQ=255) ggCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAACGGAGGCTTTTGACTATTACTCACCAGGTAAGGCACGAGGTTTTCCTTCAGGATCa > 2:2223350/1‑100 (MQ=255) gCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCaa > 2:919963/1‑100 (MQ=255) accaccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAAcc > 2:469100/1‑100 (MQ=255) accaccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAAGTAAGGCGCGAGGTTTTCCTTCAGGATCaa > 2:583921/1‑98 (MQ=255) caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgc > 1:269525/1‑100 (MQ=255) ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGATGTTTTCCTTCAGGATCAACCGCGa > 1:843765/1‑100 (MQ=255) ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAAGCTTTTGACTATTACTCAAGAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGa > 2:1304066/1‑100 (MQ=255) gAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGAAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGATTTCGTTCAGAATCAACCGCGAc > 2:804729/1‑99 (MQ=255) ttCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACt < 1:90513/100‑1 (MQ=255) aGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaa > 2:1043/1‑100 (MQ=255) aGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaa > 2:1889165/1‑100 (MQ=255) gTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaaa < 1:1378232/100‑1 (MQ=255) gTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaaa < 1:1583756/100‑1 (MQ=255) tAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaat < 1:420888/100‑1 (MQ=255) gCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaataat < 1:611465/100‑1 (MQ=255) cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATg > 1:2285927/1‑100 (MQ=255) cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATg < 2:511037/100‑1 (MQ=255) gAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCt > 1:955748/1‑100 (MQ=255) gAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCt > 1:1355286/1‑100 (MQ=255) aaaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCtt < 2:1372462/100‑1 (MQ=255) aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc < 2:765802/100‑1 (MQ=255) aGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTTAGGATCAACAGCGACATACTTAAATAATGAttat > 2:565476/1‑98 (MQ=37) gTCAAAAGCCTCCGGTCGGAGGATTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTg > 2:416009/1‑100 (MQ=255) gTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCACCCGCGACATACTTAAATAATGCTTCTg > 2:1927658/1‑100 (MQ=255) tCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGt > 1:1344064/1‑100 (MQ=255) tCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTTCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGt < 2:1323052/100‑1 (MQ=255) cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc > 1:1064100/1‑100 (MQ=255) cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc > 2:1190578/1‑100 (MQ=255) cAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc > 2:853714/1‑100 (MQ=255) cAAAAGCCTCCGACCGGAGGCTTTTGACTATTAATCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACAGCGACATACTTAAATAATGCTTCTGTc > 2:82482/1‑100 (MQ=255) naaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc > 2:2175553/2‑99 (MQ=255) aaaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc > 1:1095359/1‑99 (MQ=255) aaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGc > 1:2402115/1‑100 (MQ=255) aaaGCCTCCGACCGAAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGc > 1:2176961/1‑100 (MQ=255) gCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCttt > 1:1324667/1‑100 (MQ=255) gCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGATAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCttt > 1:1690963/1‑100 (MQ=255) gCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCttt < 1:1514776/100‑1 (MQ=255) ccTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTta < 2:2385582/100‑1 (MQ=255) ccTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTta < 1:2130771/100‑1 (MQ=255) tCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTtata < 1:921272/100‑1 (MQ=255) gACCGGTGGCTTATGACTATAACACAACAGGAAAGACACAAGGCTTTCCTTCAGGACCAACCGCGACATACTTAAATAATGTTTCCGTc > 2:359478/1‑89 (MQ=255) | AGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATA > NC_000913/1311735‑1311925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |