| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,401,844 | A→G | 100% | I471T (ATC→ACC) | abgB ← | p‑aminobenzoyl‑glutamate hydrolase, B subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,401,844 | 0 | A | G | 97.8% | 140.0 / ‑4.6 | 45 | I471T (ATC→ACC) | abgB | p‑aminobenzoyl‑glutamate hydrolase, B subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/23); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
GACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTT > NC_000913/1401751‑1401939 | gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc < 1:1772779/100‑1 (MQ=255) ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGt < 2:1231985/100‑1 (MQ=255) gTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTg < 2:4209/100‑1 (MQ=255) tGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATa > 2:1016794/1‑100 (MQ=255) tGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATa < 2:293146/100‑1 (MQ=255) ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc > 2:1883525/1‑100 (MQ=255) ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc > 2:1096419/1‑100 (MQ=255) gACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGtt > 2:1865002/1‑100 (MQ=255) gACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGtt < 1:351798/100‑1 (MQ=255) cATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGt > 1:1858537/1‑97 (MQ=255) tACTCATGGGCATTCCTCGGTTGGTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGTGTAGGGCAGTGATACGGTTGCg < 2:1863346/99‑1 (MQ=255) gggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGtt < 2:53395/100‑1 (MQ=255) ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt > 2:1235155/1‑100 (MQ=255) cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGTTAGGGCAGTGATACGGATGCGTGTCCGTTACTTGCTg > 1:149361/1‑100 (MQ=255) cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGTGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt < 2:491133/100‑1 (MQ=255) tgttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGAAGGGCAGTGATACGGCTGCGTGTCCGTTACTTGCTGATGctc > 2:1113210/1‑100 (MQ=255) gttgtGTTTGCGTTTTTGTTGTTTTTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct < 2:1071201/100‑1 (MQ=255) gtTTGCGTGGTTGTTGTTATTTTAAAGGTGACTGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgtt < 2:420338/99‑1 (MQ=255) ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc < 1:106277/100‑1 (MQ=255) tgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGcg > 1:262252/1‑99 (MQ=255) ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt < 1:1392572/100‑1 (MQ=255) ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt > 1:1993292/1‑100 (MQ=255) gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCGTTCTTGCAATAg > 1:967507/1‑100 (MQ=255) gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt > 2:1269050/1‑98 (MQ=255) ttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGc < 1:860363/100‑1 (MQ=255) tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg > 1:240470/1‑100 (MQ=255) tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg < 1:1086936/100‑1 (MQ=255) aaaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt > 2:681245/1‑100 (MQ=255) aaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt > 1:1870857/1‑99 (MQ=255) ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTTGCCTTCTTGCAATAGTCCTGAATCaa > 1:235959/1‑100 (MQ=255) gTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAg < 2:2381878/100‑1 (MQ=255) gTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAg < 1:1311559/100‑1 (MQ=255) tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg < 1:1914908/100‑1 (MQ=255) gACGGTGTCACGTTTTTCGGGGTAGGGCAGTTATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg < 2:2055138/99‑1 (MQ=255) gtgtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTGGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGaa < 1:2042439/97‑1 (MQ=255) gtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt > 2:1350058/1‑100 (MQ=255) gtCACGTTTTTCGGGGTAGAGCAGAGATACGGTTACATGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAagag > 2:560544/1‑98 (MQ=255) tCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt < 1:111904/99‑1 (MQ=255) cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg > 1:2041212/1‑100 (MQ=255) cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg < 1:1016805/100‑1 (MQ=255) ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgt < 2:2044536/100‑1 (MQ=255) ttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCGATAGCCCTGAATCAAGGAAGAGATTGACTGTgg < 2:262249/100‑1 (MQ=255) ttCGGGATAGGGCAGTGATACGGCTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGGCtgtg > 2:905872/1‑99 (MQ=255) tCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGt > 2:2016228/1‑100 (MQ=255) cGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt > 2:163685/1‑100 (MQ=255) | GACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTT > NC_000913/1401751‑1401939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |