| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,434,459 | C→T | 100% | intergenic (‑235/+499) | ynaE ← / ← ttcC | cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,434,459 | 0 | C | T | 83.4% | 21.7 / 1.2 | 18 | intergenic (‑235/+499) | ynaE/ttcC | cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (8/7); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAG > NC_000913/1434363‑1434537 | cTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTgn < 1:1283420/100‑2 (MQ=25)cTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTgg < 2:22925/100‑1 (MQ=18)cTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTg > 1:624258/1‑99 (MQ=25) gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATTTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt < 2:303685/100‑1 (MQ=255) aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTTATATAGTCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtg < 1:122659/100‑1 (MQ=255) atatGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtgt > 2:214762/1‑100 (MQ=25) tAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTaa < 2:61722/100‑1 (MQ=17) aaGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTaaa > 2:1754399/1‑100 (MQ=18) aaGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTaaa > 1:2078677/1‑100 (MQ=25) aaGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTaaa < 1:1079456/100‑1 (MQ=40) aGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAg > 2:657663/1‑100 (MQ=18) ggAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGTATTAGTAATGTGTTATATATGATATAGTTGATAATTTTAATCTTGGGTGTTCTTAAAg < 2:846840/99‑1 (MQ=11) ggAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGt > 2:1489529/1‑100 (MQ=17) gAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGtt < 2:499017/100‑1 (MQ=14) agttaaGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTATGGTGTTATATATAATATAGTTGACCATTTTTATCCTGGGTGTTCTTAACGtt < 2:1550423/95‑1 (MQ=11) aGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGTTc > 2:2221760/1‑100 (MQ=17) cGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGTTCGt > 2:617816/1‑100 (MQ=1) atatAGTTGACAATTTTTATCCTGGGTGTTCTCAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAg < 1:2317881/100‑1 (MQ=38) | CTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAG > NC_000913/1434363‑1434537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |