| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,493,774 | A→G | 20.0% | L435L (TTA→TTG) | trg → | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,493,774 | 0 | A | G | 20.0% | 120.5 / 29.1 | 65 | L435L (TTA→TTG) | trg | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Reads supporting (aligned to +/- strand): ref base A (23/29); new base G (5/8); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
ATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCG > NC_000913/1493678‑1493871 | aTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGAtt > 1:2018483/1‑100 (MQ=255)aTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAAtt < 2:1142923/100‑1 (MQ=255) tttGCCGTTGTCGCGAGCGAAGTACGGACACGCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTg < 2:1484984/100‑1 (MQ=255) tGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGACGGCTTGATCAGTGCATCAGTCAGGTTAATTGAc > 2:1261818/1‑100 (MQ=255) gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTGGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTgg > 2:1584311/1‑100 (MQ=255) gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGt > 1:659429/1‑100 (MQ=255) gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGt > 1:1374083/1‑100 (MQ=255) tCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTc < 2:298082/100‑1 (MQ=255) gCCAGCGAAGAACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCgg > 2:1689492/1‑100 (MQ=255) ccAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGa < 2:2083437/100‑1 (MQ=255) ccAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGa < 2:1898419/100‑1 (MQ=255) aGCGAAGTCAGGACACTCGGAAGTCGCAGTGCTCATGCCGCGAAAGAGATTGAAGGCTTGATCAGTGTATCAGTCAGGTTAATTGACCTGGGGTCGGa < 2:824357/98‑1 (MQ=255) aGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATg < 2:1979588/100‑1 (MQ=255) aGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATg < 2:1983048/100‑1 (MQ=255) aGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGAt > 2:1883332/1‑99 (MQ=255) gCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATg < 2:2401234/99‑1 (MQ=255) cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAg < 2:1877826/100‑1 (MQ=255) gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTgg < 1:421312/100‑1 (MQ=255) tACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGc < 1:2360038/100‑1 (MQ=255) gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACg > 2:1588529/1‑100 (MQ=255) gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACg > 2:565876/1‑100 (MQ=255) gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACg > 2:1512369/1‑100 (MQ=255) cacTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGc < 1:1968706/100‑1 (MQ=255) ctctCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTTAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGcc < 2:334868/99‑1 (MQ=255) cGCAAGTCGCAGCGCTCAGGCGGCGATAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg > 1:1462318/1‑100 (MQ=255) cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg < 1:1291099/100‑1 (MQ=255) cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg < 2:2071062/100‑1 (MQ=255) tCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAcc < 2:2039658/100‑1 (MQ=255) cGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCa > 1:1350940/1‑100 (MQ=255) ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCGGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt > 2:1785479/1‑100 (MQ=255) ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt > 2:244426/1‑100 (MQ=255) ggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTCAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgt < 2:1678199/99‑1 (MQ=255) cgAAAGGGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGa < 2:1856347/100‑1 (MQ=255) gAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGAt > 1:1191449/1‑100 (MQ=255) aaaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATg < 2:264019/100‑1 (MQ=255) aaaGAGATTGAAGGCTTGATCAGTGAATCAGGCAGGTTAATTGGCCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGa < 1:143392/98‑1 (MQ=255) nagagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGc > 2:2213338/2‑100 (MQ=255) aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGc > 1:373634/1‑100 (MQ=255) aaGAGATTGAAGGCTTGATAAATGAATAAATCAGGTTAATTGACATGAGGTCGGATAAGATAGCAACGACCGAGAAAACCATGAGCACTATTGTTAAt > 2:713479/1‑98 (MQ=255) gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCg < 1:1588549/100‑1 (MQ=255) gaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATAcc > 1:1902772/1‑97 (MQ=255) aaGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAg > 1:1314834/1‑100 (MQ=255) ggCTTGATCAGTTAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgtg < 2:2313618/100‑1 (MQ=255) cTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc < 2:373631/100‑1 (MQ=255) cTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc > 2:136211/1‑100 (MQ=255) tGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcac > 1:1759575/1‑100 (MQ=255) gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca < 1:220900/100‑1 (MQ=255) aTCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac < 2:1660834/100‑1 (MQ=255) aTCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGTAACGGCCTGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac < 2:47796/100‑1 (MQ=255) tCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacaca > 2:50060/1‑100 (MQ=255) tCAGTGAAGCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacaca < 1:453166/100‑1 (MQ=255) cAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGCGTGTCACACat > 2:1193784/1‑100 (MQ=255) cAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACat > 2:2264199/1‑100 (MQ=255) aGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata < 1:1286450/100‑1 (MQ=255) aGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata > 2:1237324/1‑100 (MQ=255) aGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata > 1:502290/1‑100 (MQ=255) tGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATc < 1:861265/100‑1 (MQ=255) tcagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc < 2:659423/100‑1 (MQ=255) tcagtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc < 2:1994262/100‑1 (MQ=255) tcagtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc < 1:785378/100‑1 (MQ=255) cagtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa > 2:2393348/1‑100 (MQ=255) gtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg < 1:513099/100‑1 (MQ=255) ggTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt < 1:1316046/100‑1 (MQ=255) ttAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg < 1:1644484/100‑1 (MQ=255) ttAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg < 1:1883354/100‑1 (MQ=255) | ATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCG > NC_000913/1493678‑1493871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |