| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,602,236 | A→T | 15.8% | Q249H (CAA→CAT) | lsrA → | autoinducer 2 import ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,602,236 | 0 | A | T | 15.8% | 145.8 / 20.6 | 57 | Q249H (CAA→CAT) | lsrA | autoinducer 2 import ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (25/23); new base T (5/4); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
ATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGT > NC_000913/1602141‑1602334 | aTCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAt < 1:1867031/100‑1 (MQ=255)aTCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAt < 1:646735/100‑1 (MQ=255) tAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATAGATCTCTGCCAGCCAAAACTTATgaat > 1:793681/1‑97 (MQ=255) aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTgg > 1:2285144/1‑100 (MQ=255) gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATATTTTTTGGCTggc < 1:1578170/100‑2 (MQ=255) gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc < 1:623802/100‑2 (MQ=255) gCGGCAAAACCAGCGAACTGTCTACCGACGACATCATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAACTTTGGGCTggc < 2:1258416/100‑2 (MQ=255) ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggcag < 2:374909/100‑4 (MQ=255) ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg > 1:1548437/1‑99 (MQ=255) ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGt > 1:565044/1‑100 (MQ=255) gCAAAACCAGCGAACTGTCTACAGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCACCCAAAAATTATGGCTGGAg > 2:1057408/1‑98 (MQ=255) cAAAACCAGCGAACTGTCTACCGACGACATTATTCAAGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGATGAAGTTa > 2:412341/1‑100 (MQ=255) aCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg < 1:134217/100‑1 (MQ=255) cAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg < 1:1450864/98‑1 (MQ=255) gAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAcc < 2:855525/100‑1 (MQ=255) tGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGccc < 1:2365466/100‑1 (MQ=255) gTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGc < 1:993871/97‑1 (MQ=255) tCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac < 2:253640/100‑1 (MQ=255) aCCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaac > 1:582099/1‑100 (MQ=255) ccGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaaca < 1:2300533/100‑1 (MQ=255) cgacgaCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTTGAGTTACCTGGTAACCGCCCACAACAt < 2:2377409/100‑1 (MQ=255) acATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGATACCTGGTAACCGCCCACAACATgccg > 2:669230/1‑99 (MQ=255) acATTATTCAGGACATCACCCCAGCGGTACGGGAAATATCGCTCTCTGCCAGCCAAAAATTATCGCTAGAGTTACCTGGTAACCGCCCACAACATgccgc > 1:1576693/1‑100 (MQ=255) cATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgcc > 1:2292311/1‑100 (MQ=255) attCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGCTACCTGGTAACCGCCCACAACATGCCGCCgg > 2:729821/1‑98 (MQ=255) tCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACg < 1:1494190/100‑1 (MQ=255) gCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCgg > 2:27893/1‑100 (MQ=255) ccATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCgg < 1:2034359/99‑1 (MQ=255) aTCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGc < 1:458541/100‑1 (MQ=255) aTCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGc < 1:1693371/100‑1 (MQ=255) tCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCt < 1:2230872/100‑1 (MQ=255) cACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg < 2:200100/100‑1 (MQ=255) aCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGa > 1:2189372/1‑100 (MQ=255) cccAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGaca > 2:917198/1‑100 (MQ=255) cAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACt > 2:1784117/1‑100 (MQ=255) gCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTCGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg < 1:1379772/100‑1 (MQ=255) ggTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGa < 2:466408/99‑1 (MQ=255) cGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATc > 1:166255/1‑100 (MQ=255) gggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCt > 1:586202/1‑100 (MQ=255) gggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCAGGAGTTACCTGGTAACCGCCCACAAAATGCAGCCGGAACGCCGGTGCTGACACTGGAAAATCt > 2:1102306/1‑100 (MQ=255) ggAAAAATCGCTCTCTGACAGCCCAACATTCCGCCTGCAGTTACACTGTAAAAGCCAAACACCTGCCGCTGGAATGCCAGTGTTTACACTGGAAAATCTg < 2:2004485/100‑1 (MQ=255) gAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTg < 2:586199/99‑1 (MQ=255) aaaaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGAc > 2:1012481/1‑100 (MQ=255) aaaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGAcc > 1:215215/1‑100 (MQ=255) aTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGc > 2:768150/1‑100 (MQ=255) gCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGaa < 2:464823/100‑1 (MQ=255) gCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGaa > 2:244355/1‑100 (MQ=255) aactccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGtt > 1:1014967/5‑100 (MQ=255) actccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACAGGAAAATCTGACCGGCGAAg > 1:615990/4‑96 (MQ=255) actccagccaTAATTTTTGGATGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGttt > 2:1513713/4‑100 (MQ=255) ccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACCCTGGAAAATCTGACAGGCGCAGGTTTCAg > 1:793118/1‑100 (MQ=255) ccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg > 1:1842205/1‑100 (MQ=255) ccagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg < 2:1956868/100‑1 (MQ=255) ccagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg > 1:832254/1‑100 (MQ=255) gccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGaa > 1:1328216/1‑100 (MQ=255) gccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGaa < 1:1054238/100‑1 (MQ=255) caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATg > 1:1169684/1‑100 (MQ=255) aaaaaTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGt > 2:1215941/1‑100 (MQ=255) | ATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGT > NC_000913/1602141‑1602334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |