| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,611,964 | T→C | 19.7% | intergenic (‑110/+2) | yneF ← / ← yneG | putative membrane‑bound diguanylate cyclase/DUF4186 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,611,964 | 0 | T | C | 19.7% | 107.2 / 24.0 | 56 | intergenic (‑110/+2) | yneF/yneG | putative membrane‑bound diguanylate cyclase/DUF4186 family protein |
| Reads supporting (aligned to +/- strand): ref base T (23/22); new base C (6/5); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CCAGACTATCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTAT > NC_000913/1611866‑1612063 | ccAGACTATCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCACATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATtc > 1:1402537/1‑100 (MQ=255)ccAGACTATCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAAtt > 2:1137533/1‑99 (MQ=255) cAGACTATCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATtct > 2:2297680/1‑100 (MQ=255) tCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCt > 2:1226539/1‑100 (MQ=255) cAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTg > 2:220878/1‑100 (MQ=255) cAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTg > 2:137338/1‑100 (MQ=255) cAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTg > 2:2244307/1‑100 (MQ=255) aaTACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGa > 1:2124454/1‑100 (MQ=255) tACTGACCGAACCAATATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGAtt < 1:2364468/100‑1 (MQ=255) aCTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTc > 2:1104488/1‑100 (MQ=255) cTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTCa > 1:840745/1‑100 (MQ=255) gACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCCTGGCTGATTCATc > 1:2114362/1‑100 (MQ=255) gACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTGGTCGGTTTCCGCAATTCTCATGGCTGCTTCATc > 2:594641/1‑100 (MQ=255) ccGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTg < 2:2124430/100‑1 (MQ=255) cGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGt < 2:663479/100‑1 (MQ=255) aaCCAAGATAGCATTTTCGCTGACAGCGGCGGCAAATAAATGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTAc < 1:1094687/100‑1 (MQ=255) ccAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACaa > 1:732508/1‑100 (MQ=255) cAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAc < 1:64907/100‑1 (MQ=255) cAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTCATCTGTACAAc < 1:237523/100‑1 (MQ=255) aaGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACACTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAcc > 2:1304824/1‑100 (MQ=255) tAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAccagcc > 2:265308/1‑100 (MQ=255) tAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAccagcc < 2:902942/100‑1 (MQ=255) tAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAccagcc < 2:1608165/100‑1 (MQ=255) gCATGTTCGCTGACAGCGGCGGCAAATAAAGGAACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAAccagccag > 2:472230/1‑100 (MQ=255) tGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGa < 1:235171/100‑1 (MQ=255) tGTTCGCTGACAGCAACAGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGa > 2:722759/1‑100 (MQ=255) tCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCCATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAg < 2:793664/100‑1 (MQ=255) gCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGAt < 2:1311734/100‑1 (MQ=255) gACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTAc > 2:1678389/1‑100 (MQ=255) cAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCg > 2:946358/1‑100 (MQ=255) cggcggCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCa > 2:2077213/1‑100 (MQ=255) ggcggcAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCAc < 1:1152840/100‑1 (MQ=255) ggcggcAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCAc < 1:1269304/100‑1 (MQ=255) gcggcAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACg > 1:306651/1‑100 (MQ=255) gcAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGata < 1:1245296/100‑1 (MQ=255) aTAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAAc < 1:1631041/100‑1 (MQ=255) aaGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACgtt > 1:2243051/1‑100 (MQ=255) tACCTTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACgttgt < 1:248662/98‑1 (MQ=255) aCCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACgttgttgt < 1:261653/100‑1 (MQ=255) gggAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGttcttc < 2:1414366/100‑1 (MQ=255) aaTGCTATTTTGTCGTGTTCCGCAATTCTCATGTCTGATTCAGCTGTACAACCAGCCAGTGATATATTACCGCCACGATATAACGTTGTTGTTCTTCGCt < 2:539340/100‑1 (MQ=255) tGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCt < 1:1403385/98‑1 (MQ=255) gCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCTTCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTaa < 2:1301500/100‑1 (MQ=255) gCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATACCGTTGTTGTTCTTCGCTTaa > 1:1608904/1‑100 (MQ=255) gCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTaa > 2:1721278/1‑100 (MQ=255) tttGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGaaa > 2:692527/1‑100 (MQ=255) tGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAaca > 1:1171686/1‑100 (MQ=255) tGTCGTTTTCCGCAATCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAaca > 1:2133455/1‑100 (MQ=255) gTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAacac > 1:701010/1‑100 (MQ=255) tCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACAcc > 1:1701346/1‑100 (MQ=255) gTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATATATTACCGCCACGATATAACGTAGTTGGTCTTCGCTTAACGATacac > 1:350785/1‑97 (MQ=255) gTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCt > 2:349619/1‑100 (MQ=255) ttttCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTg < 1:2077235/100‑1 (MQ=255) tCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCgg < 1:1226552/100‑1 (MQ=255) gCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAAt > 2:1828900/1‑100 (MQ=255) cAATCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATg < 1:2244333/100‑1 (MQ=255) aaTTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGt < 1:1398809/100‑1 (MQ=255) aaTCCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATg < 1:137341/99‑1 (MQ=255) aTTCTCATGGCTGATTCATCTGTACAACAAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGt < 2:1380484/99‑1 (MQ=255) tctcTTGGATGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTAt < 1:946369/100‑1 (MQ=255) | CCAGACTATCAATACTGACCGAACCAAGATAGCATGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTAT > NC_000913/1611866‑1612063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |