Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 232,196 G→T 17.6% G91C (GGC→TGC)  yafE → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913232,1960GT17.6% 282.5 / 45.0 115G91C (GGC→TGC) yafEputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (43/50);  new base T (11/9);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 118 total aligned reads displayed.

GCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTA  >  NC_000913/232097‑232294
                                                                                                   |                                                                                                  
gCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTg                                                                                                    <  1:410423/100‑1 (MQ=255)
 ccGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTgg                                                                                                   >  1:1920996/1‑100 (MQ=255)
 ccGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTg                                                                                                    >  2:791832/1‑99 (MQ=255)
  cGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGTATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGc                                                                                                  <  2:948638/100‑1 (MQ=255)
   gATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCg                                                                                                 >  2:955010/1‑100 (MQ=255)
   gATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCg                                                                                                 >  1:1974724/1‑100 (MQ=255)
   gATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCg                                                                                                 <  1:172514/100‑1 (MQ=255)
    aTAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGCTGCAGCACTGCGAGAAGTGAATAGGATACTGAAACCTGGcag                                                                                                <  1:1623582/100‑3 (MQ=255)
     tAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGc                                                                                                  <  1:1906581/97‑1 (MQ=255)
       aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAg                                                                                             <  2:1044131/100‑1 (MQ=255)
        cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAgg                                                                                            >  2:2085192/1‑100 (MQ=255)
        cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAgg                                                                                            <  2:619857/100‑1 (MQ=255)
        cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAgg                                                                                            >  2:823335/1‑100 (MQ=255)
         gCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGc                                                                                           <  1:145458/100‑1 (MQ=255)
          cATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAATCCTGGCGGTAGGc                                                                                           <  2:1480073/99‑1 (MQ=255)
           aTTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTg                                                                                         <  1:1492223/100‑1 (MQ=255)
           aTTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTg                                                                                         <  1:1997449/100‑1 (MQ=255)
           aTTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTg                                                                                         <  1:219366/100‑1 (MQ=255)
             ttGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGAt                                                                                       >  1:508202/1‑100 (MQ=255)
             ttGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGAt                                                                                       >  1:1004605/1‑100 (MQ=255)
               gATATTGTTATCAGTCGTTATTCTGTCAATCACTGGCATGATGTTGATGCAGCACTGCGAGAAGTGAATAGAATAGTGAAACCTGGCGGTAGGCt                                                                                          >  2:1329026/1‑95 (MQ=255)
                  atTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGa                                                                                  <  2:1154874/100‑1 (MQ=255)
                  atTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATtgtg                                                                                   <  1:323046/99‑1 (MQ=255)
                   ttGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGAt                                                                                 <  2:790815/100‑1 (MQ=255)
                     gTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCAGATTGTGATg                                                                                >  1:1472121/1‑99 (MQ=255)
                       tATCAGCCGTTATTCTGCCCATCACGGGCATGATATAGGTGCAGCACTGCGAGACGTGAATAGGCGATTAAAACCTGGCGATAGGCTGATTGTGATGGa                                                                              >  2:20128/1‑99 (MQ=255)
                        aTCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACg                                                                            <  2:140233/100‑1 (MQ=255)
                        aTCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACg                                                                            <  2:589817/100‑1 (MQ=255)
                        aTCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACg                                                                            >  1:2291172/1‑100 (MQ=255)
                         tCAGCCGTTATTCTGCCCATCACTGGCATGAGGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGt                                                                           <  2:639485/100‑1 (MQ=255)
                          cAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTa                                                                          >  1:678972/1‑100 (MQ=255)
                          cAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTa                                                                          >  2:122838/1‑100 (MQ=255)
                           aGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTaa                                                                         >  1:1841517/1‑100 (MQ=255)
                           aGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTaa                                                                         >  2:1231367/1‑100 (MQ=255)
                            gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAAt                                                                        >  2:1353919/1‑100 (MQ=255)
                            gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTaa                                                                         >  1:2275221/1‑99 (MQ=255)
                            gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAAt                                                                        <  1:2146015/100‑1 (MQ=255)
                             ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATg                                                                       <  1:540194/100‑1 (MQ=255)
                              cGTTATTCTGCCCATCACTGGCATGAAGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGt                                                                      >  1:1459907/1‑100 (MQ=255)
                                    tCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCgg                                                                >  2:529651/1‑100 (MQ=255)
                                    tCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGGGATGGACGTAATGTCTCCgg                                                                <  2:1459891/100‑1 (MQ=255)
                                       gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTCATGTCTACGGGTc                                                             >  1:610991/1‑100 (MQ=255)
                                       gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGt                                                              >  1:870731/1‑99 (MQ=255)
                                         ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAc                                                           <  1:734810/100‑1 (MQ=255)
                                             cACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAg                                                       <  1:715937/100‑1 (MQ=255)
                                             cACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAg                                                       <  1:716436/100‑1 (MQ=255)
                                             cACTGGCATGATGCTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAg                                                       >  1:1306213/1‑100 (MQ=255)
                                               cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTg                                                     <  2:2101219/100‑1 (MQ=255)
                                                 ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgc                                                    >  1:1222949/1‑99 (MQ=255)
                                                 ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATAGTGATGGACGTAATGTCTCCGGGTCACTCAGTgcg                                                   >  2:1088456/1‑100 (MQ=255)
                                                  gCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcgc                                                  >  2:961495/1‑100 (MQ=255)
                                                   cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                                 <  1:2392905/100‑1 (MQ=255)
                                                   cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTTTCCGGGTCACCCAGTGcgcg                                                 <  2:142217/100‑1 (MQ=255)
                                                   cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                                 <  2:874211/100‑1 (MQ=255)
                                                   cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                                 <  2:874037/100‑1 (MQ=255)
                                                     tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGAc                                               <  1:1686292/100‑1 (MQ=255)
                                                     tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGAc                                               >  1:222830/1‑100 (MQ=255)
                                                        tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCCGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATc                                            <  2:1254236/100‑1 (MQ=255)
                                                             gTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                       <  1:405384/100‑1 (MQ=255)
                                                             gTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                       >  2:422014/1‑100 (MQ=255)
                                                             gTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                       >  2:1584900/1‑100 (MQ=255)
                                                              tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                      >  1:783074/1‑100 (MQ=255)
                                                              tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                      >  2:111335/1‑100 (MQ=255)
                                                              tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                      <  1:1603801/100‑1 (MQ=255)
                                                              tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                      <  1:2206172/100‑1 (MQ=255)
                                                               gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTaa                                     >  2:1603781/1‑99 (MQ=255)
                                                                cagcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                    <  1:1231380/100‑1 (MQ=255)
                                                                 agcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGATTACAg                                   >  2:1892190/1‑100 (MQ=255)
                                                                 agcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg                                   <  2:2192813/100‑1 (MQ=255)
                                                                 agcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg                                   <  2:2192758/100‑1 (MQ=255)
                                                                  gcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                  >  1:974228/1‑100 (MQ=255)
                                                                   caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTTCGCGACATCTGGTTACAGAc                                 <  2:196204/100‑1 (MQ=255)
                                                                    aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACg                                >  1:206700/1‑100 (MQ=255)
                                                                     cTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACgg                               >  2:796111/1‑100 (MQ=255)
                                                                       gCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                             >  2:1026055/1‑100 (MQ=255)
                                                                          agaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                          >  2:1885817/1‑100 (MQ=255)
                                                                          agaAGTGAATAGGATATTGAAACCTGGCCGTAGGCTTATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                          <  1:2138457/100‑1 (MQ=255)
                                                                           gaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAg                         <  1:1270651/100‑1 (MQ=255)
                                                                           gaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                           >  1:313905/1‑98 (MQ=255)
                                                                           gaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAg                         <  1:1290511/100‑1 (MQ=255)
                                                                            aaGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                        <  2:452248/100‑1 (MQ=255)
                                                                                naaTAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGGCATCTGGTTACAGACGGTAGAAGCAt                      >  2:596073/2‑98 (MQ=255)
                                                                                  aTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcg                  <  1:2051454/100‑1 (MQ=255)
                                                                                   tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc                 >  1:1304084/1‑100 (MQ=255)
                                                                                   tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc                 <  1:274052/100‑1 (MQ=255)
                                                                                   tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc                 <  2:1927271/100‑1 (MQ=255)
                                                                                    aGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgcg                >  2:166039/1‑100 (MQ=255)
                                                                                     ngATATTGAAACCTGGCGGTAGGCTGATTGTGAGGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATATGGTTACAGACGGTAGAAGCATTACGCGa               >  2:1437420/2‑100 (MQ=255)
                                                                                      gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt              <  2:957973/100‑1 (MQ=255)
                                                                                      gATATTGAAACCTAGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt              >  1:1618443/1‑100 (MQ=255)
                                                                                        tatTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAc            >  1:2273784/1‑100 (MQ=255)
                                                                                         atTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc           >  1:2090006/1‑100 (MQ=255)
                                                                                           tctaaCCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc         <  2:1886005/97‑1 (MQ=255)
                                                                                           tGAAACCTGGCGGTAGGCTGATAGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGAAATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc         >  2:122211/1‑100 (MQ=255)
                                                                                            gAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct        <  2:288706/100‑1 (MQ=255)
                                                                                            gAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct        >  2:2170453/1‑100 (MQ=255)
                                                                                              aaCCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctcaga      >  1:1886027/1‑97 (MQ=255)
                                                                                              aaCCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa      <  2:928384/100‑1 (MQ=255)
                                                                                                ccTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACg    <  1:342542/100‑1 (MQ=255)
                                                                                                  tGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTa  <  1:1364222/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTA  >  NC_000913/232097‑232294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: