| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,964,425 | G→C | 17.3% | A209G (GCC→GGC) | flhA ← | putative flagellar export pore protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,964,425 | 0 | G | C | 17.3% | 141.9 / 26.6 | 58 | A209G (GCC→GGC) | flhA | putative flagellar export pore protein |
| Reads supporting (aligned to +/- strand): ref base G (23/25); new base C (6/4); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01 | |||||||||||
TAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCA > NC_000913/1964326‑1964523 | taACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATgg < 1:102301/100‑1 (MQ=255)taACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATgg < 1:1288737/100‑1 (MQ=255) aaCTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGc > 2:1824820/1‑100 (MQ=255) aaCTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGc < 1:274102/100‑1 (MQ=255) aCTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCa < 1:576598/100‑1 (MQ=255) cTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCAt > 2:1419544/1‑100 (MQ=255) ccgccgCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGCCATcgcc < 1:756343/100‑1 (MQ=255) cgccgcGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATcgccg > 2:1908755/1‑100 (MQ=255) gccgcGTGTCCCATTCTCATGCCATGGTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGCCATcgccgg < 2:1716320/100‑2 (MQ=255) gccgcGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGCCATcgccgg < 1:2156223/100‑2 (MQ=255) ccgcGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCcgcg < 1:2089776/100‑1 (MQ=255) ccgcGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGCCATcgccggg < 1:494231/100‑3 (MQ=255) ccgcGTGTCCCATGCGCAGGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCcgcg < 1:1292432/100‑1 (MQ=255) cgTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAAc < 2:2176033/100‑1 (MQ=255) tCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAac < 1:1957595/100‑1 (MQ=255) cccATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAact < 1:2018487/100‑1 (MQ=255) cccATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAact > 1:2303174/1‑100 (MQ=255) cccATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAac > 1:1661538/1‑99 (MQ=255) ccATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAactt > 2:2219212/1‑100 (MQ=255) ccATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAactt < 2:734001/100‑1 (MQ=255) cATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAactta > 1:1493483/1‑100 (MQ=255) gCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAacttactt > 2:64856/1‑100 (MQ=255) cATGCCATGTTGCAGCACGCCGACCAGCAACCCGTCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGcc < 2:12304/100‑1 (MQ=255) gCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCg > 2:417248/1‑100 (MQ=255) ccATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGt > 1:1284074/1‑100 (MQ=255) tGCAGCACGCCGACCAGCAACCCGCCAACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTACCCCGTCCAt > 1:1454431/1‑98 (MQ=255) agcaCGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGc < 1:2383297/100‑1 (MQ=255) agcaCGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGc < 2:1445085/100‑1 (MQ=255) gcaCGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGcc < 1:2219238/100‑1 (MQ=255) caCTCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGATTCGTGGCGATGGCATCGACGCGAACAAACTTACTTGCCCCGTCCATTGAGCCg < 2:843317/100‑1 (MQ=255) caCGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCg < 2:746729/100‑1 (MQ=255) aCGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGt > 1:1022042/1‑100 (MQ=255) gCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTaa > 1:688936/1‑100 (MQ=255) ccAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGTGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATc > 1:2384320/1‑100 (MQ=255) cAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCg < 1:1714509/100‑1 (MQ=255) cAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCg < 2:1853655/100‑1 (MQ=255) aGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCgg < 2:569275/100‑1 (MQ=255) gCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGc > 2:500576/1‑100 (MQ=255) cccGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACCAAATTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTcc > 2:1688938/1‑100 (MQ=255) ccgccgACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCt < 2:520460/100‑1 (MQ=255) cgccgACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTg < 2:2361753/100‑1 (MQ=255) ccgACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAg < 1:1174086/100‑1 (MQ=255) gTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACATCg > 1:322416/1‑100 (MQ=255) tAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCgg > 2:2315095/1‑99 (MQ=255) ccATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAgcggcg > 1:2050285/1‑100 (MQ=255) gatgaGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGtttt > 1:169375/1‑100 (MQ=255) atgaGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGttttt > 1:1681045/1‑100 (MQ=255) ggATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGc > 1:1624026/1‑100 (MQ=255) gATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGcc < 1:2390245/100‑1 (MQ=255) ttcgcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCt > 1:231346/5‑100 (MQ=255) aTCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCt < 1:924097/100‑1 (MQ=255) cgcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCa > 2:1788826/3‑100 (MQ=255) cgcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCa > 1:1131268/3‑100 (MQ=255) cgcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCa > 2:1788662/3‑100 (MQ=255) cgcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCa > 1:1923195/3‑100 (MQ=255) gcGGCGATGCCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCAt > 1:1265759/2‑100 (MQ=255) ccGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCan < 1:772670/100‑2 (MQ=255) ccGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCAt > 1:605619/1‑100 (MQ=255) aTGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCa < 2:1934526/99‑1 (MQ=255) tGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCAcc < 1:777210/100‑1 (MQ=255) ggCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACca > 2:1397238/1‑100 (MQ=255) ggCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACca < 2:987954/100‑1 (MQ=255) | TAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCA > NC_000913/1964326‑1964523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |