Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,971,761 | T→C | 16.0% | T311A (ACC→GCC) | tar ← | methyl‑accepting chemotaxis protein II |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,971,761 | 0 | T | C | 16.0% | 173.6 / 25.9 | 81 | T311A (ACC→GCC) | tar | methyl‑accepting chemotaxis protein II |
Reads supporting (aligned to +/- strand): ref base T (33/35); new base C (9/4); total (42/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 |
ATCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAC > NC_000913/1971663‑1971859 | aTCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGTCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTg < 2:710613/100‑1 (MQ=255) aTCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTg < 1:2312538/100‑1 (MQ=255) ccaccaCTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAg < 2:1989048/100‑1 (MQ=255) caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgc > 2:1184550/1‑100 (MQ=255) caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgc < 1:2370953/100‑1 (MQ=255) accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgct < 1:776405/100‑1 (MQ=255) accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgct > 1:1178006/1‑100 (MQ=255) acTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgctgct < 1:1351597/100‑1 (MQ=255) cTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTc < 1:1042600/100‑1 (MQ=255) gccgccGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCagg < 1:1622503/100‑3 (MQ=255) ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGc > 1:457721/1‑100 (MQ=255) cgccgTGCTGGGCGGTGTCGGAGGCCGTTTGCGCTAGTTGCGAGGTCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATg < 2:1017519/98‑1 (MQ=255) cgTGCTGGGCGGTGTCGGAGGCACTTTGCGTCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGc < 2:675982/96‑1 (MQ=255) cgTGCTGGGCGGTGACGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGTCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggc < 2:1633993/100‑1 (MQ=255) tggtGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGGGGCCTGGCGGGGGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcgg < 2:2189240/97‑1 (MQ=255) tGCTGGGCGGTGTCGGTGGCACTTTGCGCCAGTTGGGAGGCGTGGCGGGCGTTATCTGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGc < 2:72390/94‑1 (MQ=255) tGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcgg > 2:530750/1‑100 (MQ=255) tGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcgg > 1:1603361/1‑100 (MQ=255) gggCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGt > 1:1205407/1‑100 (MQ=255) ggCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGtt < 2:1128699/100‑1 (MQ=255) cGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttc < 1:1339214/100‑1 (MQ=255) cGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttc < 2:1623042/100‑1 (MQ=255) tgtCGGAGGCACGTTGCGCCAGTTGCGAGGCTTGGCGGGCTTGATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTTCATGCTGGCGGCAGTttctt < 2:931997/99‑1 (MQ=38) gtCGGAGGCACTTTGCGCCAGTTGCGAAGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttctttc > 2:900150/1‑98 (MQ=255) cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg < 2:1758284/100‑1 (MQ=255) ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc > 2:664752/1‑100 (MQ=255) cACTTTGCGCCGGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg > 2:1551709/1‑99 (MQ=255) cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa < 2:1205396/100‑1 (MQ=255) cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa > 1:2380387/1‑100 (MQ=255) aCTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCGTCCAGCGCgg > 2:1959948/1‑98 (MQ=255) tGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGcc > 2:501266/1‑99 (MQ=255) cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc < 1:208875/100‑1 (MQ=255) gcCAGTTGCGAGGCCTAACGGGCGTTAACGGCGTATTGATTCACGGTCGCCGTGAGCTGCTCCATGCTGGCGGGAGTTTCTTCCAGCGCGGATTCcggct > 2:793227/1‑100 (MQ=25) cAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGt < 2:478565/100‑1 (MQ=255) ttGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCa < 1:1277973/100‑1 (MQ=255) ttGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCa < 2:2402664/100‑1 (MQ=255) tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg > 1:63802/1‑100 (MQ=255) tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg < 1:223710/100‑1 (MQ=255) tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg > 2:2335478/1‑100 (MQ=255) gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt > 1:1642892/1‑100 (MQ=255) cGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTa > 1:2218825/1‑100 (MQ=255) gAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTAc > 1:1520349/1‑100 (MQ=255) cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag < 2:1886425/100‑1 (MQ=255) tGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggagg > 2:2228039/1‑100 (MQ=255) ggcgggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCCGCGCGGATGCCTGCTGTTCAGTACGagagga > 1:767730/1‑100 (MQ=255) gggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg < 1:95650/100‑1 (MQ=255) gggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg > 2:2380748/1‑100 (MQ=255) gggcgTTATCGGAGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg > 1:1576602/1‑100 (MQ=255) ggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGa > 2:1359688/1‑100 (MQ=255) ggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGa < 1:1623953/100‑1 (MQ=255) gcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGAt > 1:153734/1‑100 (MQ=255) cgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc < 2:542207/100‑1 (MQ=255) tATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgt < 2:359644/100‑1 (MQ=255) aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg > 2:1600681/1‑100 (MQ=255) aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg > 2:689330/1‑100 (MQ=255) tCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt < 2:1265303/100‑1 (MQ=255) tCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt > 1:1355893/1‑100 (MQ=255) tCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGGTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt < 2:1666554/100‑1 (MQ=255) tCGGCGTTTTGATTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGCTGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg < 1:434928/99‑1 (MQ=255) cGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt > 2:2088378/1‑100 (MQ=255) ggCGTTTTGCTTCACTGTCGCGGTGAGGTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt < 2:1662663/99‑1 (MQ=255) ggCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTg < 2:12387/100‑1 (MQ=255) cGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGcc > 1:1382303/1‑100 (MQ=255) gTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGTTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATAGGTGTTGccc > 1:140866/1‑100 (MQ=255) tttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGCTGccccc > 2:1148853/1‑98 (MQ=255) ttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgcc > 1:2354337/1‑100 (MQ=255) ttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgcc < 2:248085/100‑1 (MQ=255) tGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg < 2:50369/100‑1 (MQ=255) gCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc > 1:2331812/1‑100 (MQ=255) gCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc > 2:1775686/1‑100 (MQ=255) cTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGGTGCCTGCTGTTCAGTACGGGGGGAAAGATCGGTGTTGCCCGCCGCa > 1:1827201/1‑100 (MQ=255) tCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt < 1:1107690/100‑1 (MQ=255) aCTTTCGCGCTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAttt < 2:932472/100‑1 (MQ=255) gTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCAc > 1:2212994/1‑100 (MQ=255) cgcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg > 2:2044980/1‑100 (MQ=255) cgcgGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg > 2:1371410/1‑100 (MQ=255) gcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg > 2:1247214/1‑100 (MQ=255) gcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg < 1:2326257/100‑1 (MQ=255) gTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAc > 1:2115638/1‑100 (MQ=255) gTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAc > 1:1530334/1‑100 (MQ=255) gTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAc > 1:1329372/1‑100 (MQ=255) | ATCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAC > NC_000913/1971663‑1971859 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |