Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,159,354 | C→T | 15.4% | R323R (CGC→CGT) | mdtC → | multidrug efflux system, subunit C |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,159,354 | 0 | C | T | 15.4% | 132.1 / 13.8 | 58 | R323R (CGC→CGT) | mdtC | multidrug efflux system, subunit C |
Reads supporting (aligned to +/- strand): ref base C (21/27); new base T (5/4); total (27/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 |
CCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCT > NC_000913/2159257‑2159448 | ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTc < 1:1785506/100‑1 (MQ=255) aaTATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccc < 1:884300/100‑1 (MQ=255) atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTcccc < 1:989523/100‑1 (MQ=255) tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGAAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacc > 2:466931/1‑100 (MQ=255) atCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacca > 1:2111491/1‑100 (MQ=255) acAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCTTTCCTGCGGCGAGTGATCTGCAAATTGCCCAGGATTGGTCCTccacca > 2:197158/2‑98 (MQ=255) cGGGTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCATGATCGCTCCCCCACCATTcgcg < 2:1386058/99‑1 (MQ=255) ttGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTc < 2:2271251/100‑1 (MQ=255) gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGc > 2:568408/1‑100 (MQ=255) aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCCGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCt < 2:1182802/100‑1 (MQ=255) aGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTgg < 2:595572/100‑1 (MQ=255) gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGga > 1:324404/1‑100 (MQ=255) cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaa < 1:1636406/100‑1 (MQ=255) aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag < 2:207105/100‑1 (MQ=255) tCCGGGCAAAATTACCGGAGTTGCATGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga < 2:1098347/100‑1 (MQ=255) tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga > 1:254756/1‑100 (MQ=255) tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga > 2:37597/1‑100 (MQ=255) ggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTc < 2:250222/100‑1 (MQ=255) cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa < 2:2047549/100‑1 (MQ=255) aaaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAg < 1:2100903/100‑1 (MQ=255) aaaaTGACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCTCCATTCGCGCCTCGCTGGAAGAAGTCGAg < 2:528022/100‑1 (MQ=255) aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc < 2:568004/100‑1 (MQ=255) aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCTTCGCTGGAAGAAGTCGAGCa < 2:301693/100‑1 (MQ=255) aCCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACg > 2:1481163/1‑100 (MQ=255) ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc > 2:69379/1‑100 (MQ=255) tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt > 2:1200772/1‑100 (MQ=255) tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt > 1:944675/1‑100 (MQ=255) gCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtc > 2:8950/1‑100 (MQ=255) cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct > 2:269450/1‑100 (MQ=255) aGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtctc < 1:405038/100‑1 (MQ=255) ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg > 1:1050853/1‑100 (MQ=255) ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg > 2:2391214/1‑100 (MQ=255) aCCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCTCCTCGCTGTAAGAAGTCGAGCAAACGCTGATTATCTCgg < 2:2149473/97‑1 (MQ=255) ccATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTgg < 2:1771384/99‑1 (MQ=255) cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg > 2:1069478/1‑100 (MQ=255) tCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTg < 2:944664/100‑1 (MQ=255) ggcggcGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt > 2:677565/1‑99 (MQ=255) ggcggaGATGGATGTGCACATTGCGCAGGATCGTGCCCCGCACATTCGCGCGTCGCTGGAAGAAGTCGAGCGAACGCTGATTATCTCGGTGGCGCTGGt < 2:2298727/99‑1 (MQ=255) cggcgATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGa < 1:841601/99‑1 (MQ=255) cgATTGATCTGCAAATTGCCCAGGCTCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt < 1:1069488/100‑1 (MQ=255) cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt > 2:1755062/1‑100 (MQ=255) tCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg > 1:1804208/1‑100 (MQ=255) tCTGCAAATTGCACAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg < 1:839123/100‑1 (MQ=255) cTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGCTACTGgtggtg > 2:271405/1‑99 (MQ=255) cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt > 2:1547065/1‑92 (MQ=255) cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGgtgt < 1:1562784/100‑1 (MQ=255) tgctgaTTGGCCAGGATTGCTCCCCCACGTCTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt < 2:1860905/95‑1 (MQ=255) tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg > 2:1497080/1‑98 (MQ=255) tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt < 1:1658619/100‑1 (MQ=255) cAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt < 1:1663397/100‑1 (MQ=255) aaaTTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttttt < 1:466934/100‑1 (MQ=255) tGCCGAGGATCGTTCCCCCACCATTCGCGCATCGCTGGTAGAAGTCGAGCAAGCGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAtt < 2:609544/99‑1 (MQ=255) gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGTCTCTGAAGAAGATTTTATTcc > 2:508965/1‑100 (MQ=255) cccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCt > 1:1164403/1‑100 (MQ=255) ccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGACTCTGGAGATATTTTTATTcat > 2:1961850/1‑97 (MQ=255) cAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgc < 2:2111467/100‑1 (MQ=255) aGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgcg > 1:2083819/1‑100 (MQ=255) aTCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCt > 1:1105964/1‑99 (MQ=255) | CCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCT > NC_000913/2159257‑2159448 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |