Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,159,354 C→T 15.4% R323R (CGC→CGT mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,159,3540CT15.4% 132.1 / 13.8 58R323R (CGC→CGTmdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (21/27);  new base T (5/4);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

CCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCT  >  NC_000913/2159257‑2159448
                                                                                                 |                                                                                              
ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTc                                                                                              <  1:1785506/100‑1 (MQ=255)
  aaTATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccc                                                                                            <  1:884300/100‑1 (MQ=255)
   atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTcccc                                                                                           <  1:989523/100‑1 (MQ=255)
       tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGAAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacc                                                                                       >  2:466931/1‑100 (MQ=255)
        atCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacca                                                                                      >  1:2111491/1‑100 (MQ=255)
          acAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCTTTCCTGCGGCGAGTGATCTGCAAATTGCCCAGGATTGGTCCTccacca                                                                                      >  2:197158/2‑98 (MQ=255)
               cGGGTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCATGATCGCTCCCCCACCATTcgcg                                                                                <  2:1386058/99‑1 (MQ=255)
                  ttGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTc                                                                            <  2:2271251/100‑1 (MQ=255)
                    gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGc                                                                          >  2:568408/1‑100 (MQ=255)
                     aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCCGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCt                                                                         <  2:1182802/100‑1 (MQ=255)
                       aGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTgg                                                                       <  2:595572/100‑1 (MQ=255)
                        gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGga                                                                      >  1:324404/1‑100 (MQ=255)
                         cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaa                                                                     <  1:1636406/100‑1 (MQ=255)
                          aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                    <  2:207105/100‑1 (MQ=255)
                           tCCGGGCAAAATTACCGGAGTTGCATGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga                                                                   <  2:1098347/100‑1 (MQ=255)
                           tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga                                                                   >  1:254756/1‑100 (MQ=255)
                           tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaaga                                                                   >  2:37597/1‑100 (MQ=255)
                               ggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTc                                                               <  2:250222/100‑1 (MQ=255)
                                 cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                             <  2:2047549/100‑1 (MQ=255)
                                  aaaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAg                                                            <  1:2100903/100‑1 (MQ=255)
                                  aaaaTGACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCTCCATTCGCGCCTCGCTGGAAGAAGTCGAg                                                            <  2:528022/100‑1 (MQ=255)
                                   aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                           <  2:568004/100‑1 (MQ=255)
                                    aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCTTCGCTGGAAGAAGTCGAGCa                                                          <  2:301693/100‑1 (MQ=255)
                                        aCCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACg                                                      >  2:1481163/1‑100 (MQ=255)
                                         ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc                                                     >  2:69379/1‑100 (MQ=255)
                                                tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                              >  2:1200772/1‑100 (MQ=255)
                                                tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                              >  1:944675/1‑100 (MQ=255)
                                                 gCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtc                                             >  2:8950/1‑100 (MQ=255)
                                                  cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct                                            >  2:269450/1‑100 (MQ=255)
                                                   aGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtctc                                           <  1:405038/100‑1 (MQ=255)
                                                    ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                          >  1:1050853/1‑100 (MQ=255)
                                                    ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                          >  2:2391214/1‑100 (MQ=255)
                                                        aCCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCTCCTCGCTGTAAGAAGTCGAGCAAACGCTGATTATCTCgg                                         <  2:2149473/97‑1 (MQ=255)
                                                         ccATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTgg                                      <  2:1771384/99‑1 (MQ=255)
                                                          cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg                                    >  2:1069478/1‑100 (MQ=255)
                                                             tCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTg                                 <  2:944664/100‑1 (MQ=255)
                                                                ggcggcGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                               >  2:677565/1‑99 (MQ=255)
                                                                ggcggaGATGGATGTGCACATTGCGCAGGATCGTGCCCCGCACATTCGCGCGTCGCTGGAAGAAGTCGAGCGAACGCTGATTATCTCGGTGGCGCTGGt                               <  2:2298727/99‑1 (MQ=255)
                                                                  cggcgATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGa                             <  1:841601/99‑1 (MQ=255)
                                                                     cgATTGATCTGCAAATTGCCCAGGCTCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                         <  1:1069488/100‑1 (MQ=255)
                                                                     cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                         >  2:1755062/1‑100 (MQ=255)
                                                                            tCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                  >  1:1804208/1‑100 (MQ=255)
                                                                            tCTGCAAATTGCACAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                  <  1:839123/100‑1 (MQ=255)
                                                                             cTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGCTACTGgtggtg                  >  2:271405/1‑99 (MQ=255)
                                                                             cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                         >  2:1547065/1‑92 (MQ=255)
                                                                             cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGgtgt                 <  1:1562784/100‑1 (MQ=255)
                                                                              tgctgaTTGGCCAGGATTGCTCCCCCACGTCTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                <  2:1860905/95‑1 (MQ=255)
                                                                              tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                  >  2:1497080/1‑98 (MQ=255)
                                                                              tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                <  1:1658619/100‑1 (MQ=255)
                                                                                cAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt              <  1:1663397/100‑1 (MQ=255)
                                                                                 aaaTTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttttt             <  1:466934/100‑1 (MQ=255)
                                                                                     tGCCGAGGATCGTTCCCCCACCATTCGCGCATCGCTGGTAGAAGTCGAGCAAGCGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAtt          <  2:609544/99‑1 (MQ=255)
                                                                                      gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGTCTCTGAAGAAGATTTTATTcc        >  2:508965/1‑100 (MQ=255)
                                                                                       cccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCt       >  1:1164403/1‑100 (MQ=255)
                                                                                        ccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGACTCTGGAGATATTTTTATTcat       >  2:1961850/1‑97 (MQ=255)
                                                                                         cAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgc     <  2:2111467/100‑1 (MQ=255)
                                                                                          aGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgcg    >  1:2083819/1‑100 (MQ=255)
                                                                                             aTCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCt  >  1:1105964/1‑99 (MQ=255)
                                                                                                 |                                                                                              
CCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCT  >  NC_000913/2159257‑2159448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: