Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,291,084 | A→C | 51.4% | K195N (AAA→AAC) | narP → | response regulator in two‑component regulatory system with NarQ |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,291,084 | 0 | A | C | 51.4% | 12.2 / 98.1 | 73 | K195N (AAA→AAC) | narP | response regulator in two‑component regulatory system with NarQ |
Reads supporting (aligned to +/- strand): ref base A (20/15); new base C (23/14); total (44/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.10e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 |
ACGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGATT > NC_000913/2290987‑2291182 | aCGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACt < 2:101742/100‑1 (MQ=255) gAGGTGGCGGAGGGGGTGTCAAATAAACAGAGTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCt < 2:2146694/98‑1 (MQ=255) gCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAAt < 1:578891/100‑1 (MQ=255) cTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATg < 2:2317731/100‑1 (MQ=255) cTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATg < 2:368879/100‑1 (MQ=255) cacaGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCg > 2:318950/1‑100 (MQ=255) tgggCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCa > 2:273287/2‑100 (MQ=255) ggggCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCa > 1:302372/1‑100 (MQ=255) ggggCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCa < 2:947050/100‑1 (MQ=255) ggggCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCa > 2:1977532/1‑100 (MQ=255) gCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCAcgc > 2:1723293/1‑100 (MQ=255) gCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCAcgc > 2:1635645/1‑100 (MQ=255) gCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCAcgc > 1:452288/1‑100 (MQ=255) cTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCAcgcg < 2:2327587/100‑1 (MQ=255) tGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGt > 2:529159/1‑100 (MQ=255) gTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCAcgc > 2:1551388/1‑97 (MQ=255) gTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTg < 1:2239172/100‑1 (MQ=255) tCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGGCCGCTCACGCGTg < 2:2160291/99‑1 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTggc > 2:2254922/1‑100 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACACGTagc > 1:33556/1‑100 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCATggc > 1:1311731/1‑100 (MQ=255) aaaTAAACAGATTGCCTCAGAGTTGAATATTACCGAGAAGACAGTAAAAGTACATATTAGCAATCTGATGCGTAATCTCAATGTCCGCTCACGCGTggcg > 2:2059391/1‑100 (MQ=255) aaTAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAATTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTggcgg < 1:577571/100‑1 (MQ=255) aTAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTggcggc > 1:1853203/1‑100 (MQ=255) tAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGcc > 1:1700685/1‑100 (MQ=255) tAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGcc < 2:194922/100‑1 (MQ=255) tAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGcc > 2:1587863/1‑100 (MQ=255) aaaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGcca < 1:1695992/100‑1 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccac < 2:1551204/100‑1 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccac > 2:1506674/1‑100 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGccac > 1:2347081/1‑100 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGccac < 2:1051339/100‑1 (MQ=255) aGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCAt > 2:286629/1‑100 (MQ=255) gATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCAtt > 2:1963346/1‑100 (MQ=255) aTTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTc < 2:1087707/100‑1 (MQ=255) cTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTc > 1:831547/1‑100 (MQ=255) cTCGGTGTTGAATATTTCCAAGCAGACAGTAAAAGTACATATTCGCAATCTGCTCCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTc < 2:2263259/100‑1 (MQ=255) tctgtgtTGAATATTTCCGAGCAGACATTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTcc < 2:2134262/97‑1 (MQ=255) tgtTGAATATTTCCGAGCAGACAGTAAAAGTATATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGca < 2:2240115/100‑1 (MQ=255) tgtTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTg < 2:2100244/98‑1 (MQ=255) ttGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaac > 1:2156330/1‑100 (MQ=255) tGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaaca > 1:2248310/1‑100 (MQ=255) tGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaaca > 1:274991/1‑100 (MQ=255) gAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaacaa > 1:19859/1‑100 (MQ=255) aaTATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTCGCGGCCACCATTCTGTTCCTGCaacaac > 1:823294/1‑100 (MQ=255) aaTATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCaacaac > 2:985253/1‑100 (MQ=255) tttCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCgg > 1:1872554/1‑100 (MQ=255) gAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGcac > 2:66944/1‑100 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaa > 2:1417349/1‑100 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaa > 1:1366621/1‑100 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaa > 1:172448/1‑100 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaa < 2:1585196/100‑1 (MQ=255) cAGTAAAAGTACCTATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaaa < 2:2405784/99‑1 (MQ=255) cAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaaaa > 2:1269241/1‑100 (MQ=255) gTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGACCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAAt > 2:2168011/1‑100 (MQ=255) tAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATa < 2:1637489/100‑1 (MQ=255) aaaaGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAg > 2:743586/1‑100 (MQ=255) aaaaGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAg < 1:252564/100‑1 (MQ=255) aaGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGcc < 1:1351468/100‑1 (MQ=255) aGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGccc > 2:540598/1‑100 (MQ=255) aCATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGAt > 1:679016/1‑100 (MQ=255) tatTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGa > 1:1585174/1‑100 (MQ=255) tatTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGa > 2:1609904/1‑100 (MQ=255) ttCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGAtt < 2:1136977/100‑1 (MQ=255) ttCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGa > 2:448909/1‑98 (MQ=255) ttCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGAtt > 1:1530762/1‑100 (MQ=255) tCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGAttt < 2:1872532/100‑1 (MQ=255) cGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTa > 2:1998392/1‑100 (MQ=255) gCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTat > 1:1354549/1‑100 (MQ=255) aTCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTACTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTatc < 1:946675/98‑1 (MQ=255) ctgcggCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTACCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCg < 2:1181864/100‑1 (MQ=255) ctgcGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGc < 2:1867451/100‑1 (MQ=255) gTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTgag > 2:929606/1‑100 (MQ=255) aaaCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGAt > 2:1644749/1‑100 (MQ=255) aaCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGAtt > 1:1192937/1‑100 (MQ=255) | ACGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGATT > NC_000913/2290987‑2291182 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |