| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,352,993 | T→A | 67.8% | I116N (ATC→AAC) | glpA → | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,352,993 | 0 | T | A | 67.8% | 109.8 / 68.6 | 84 | I116N (ATC→AAC) | glpA | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/13); new base A (27/29); total (42/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTG > NC_000913/2352897‑2353091 | aCTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg < 1:1206555/100‑1 (MQ=255) gCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAgcgc < 2:987823/100‑1 (MQ=255) gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAg > 1:381702/1‑100 (MQ=255) gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAg > 1:1075068/1‑100 (MQ=255) aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGc > 1:859217/1‑100 (MQ=255) ccaaccatCGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCta < 2:550035/100‑1 (MQ=255) caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGc < 1:811444/97‑1 (MQ=255) caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtat < 1:830298/100‑1 (MQ=255) atccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCt < 1:522898/95‑1 (MQ=255) aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCACCGCAGAAGCtata > 2:2304974/1‑100 (MQ=255) aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata > 1:1920417/1‑100 (MQ=255) aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata < 1:284544/100‑1 (MQ=255) caacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAc < 2:795027/100‑1 (MQ=255) gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgca < 2:1075058/100‑1 (MQ=255) tGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgcagca < 2:1243513/100‑1 (MQ=255) tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAg < 1:1481083/100‑1 (MQ=255) ttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgc < 1:2193092/100‑1 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcg < 1:773836/100‑1 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcg > 1:997037/1‑100 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCCCATAAGCTATAGACCCgcagca > 2:140060/1‑96 (MQ=255) taaCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTAGTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg > 1:1623862/3‑100 (MQ=255) aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcgcgc > 2:1732454/1‑99 (MQ=255) aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa > 2:1733469/1‑100 (MQ=255) cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt > 2:1327636/1‑100 (MQ=255) cccTGCCGGAAGATGACCTCTCCTTCCAGACCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt > 2:1005921/1‑100 (MQ=255) ccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt < 2:633053/100‑1 (MQ=255) cGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAATAATCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGTGCGCATTATc > 2:1683977/1‑98 (MQ=255) gAAGATGACCCCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAATCTATAGACCCGCAGCAAGCGCGCATTATCGAAc < 2:1571641/100‑1 (MQ=255) aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAcc < 2:1174469/100‑1 (MQ=255) aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAcc > 2:1560808/1‑100 (MQ=255) aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAACTATAGAACCGCAGCAAGCGCGCATTATCGaa > 1:1378434/1‑98 (MQ=255) aGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCt < 1:1652202/100‑1 (MQ=255) gATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTg > 1:211196/1‑100 (MQ=255) aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGACCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc > 2:2254386/1‑100 (MQ=255) aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc < 1:1495674/100‑1 (MQ=255) tGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGcc < 1:1603981/100‑1 (MQ=255) tGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGcc > 1:1124994/1‑100 (MQ=255) aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt > 2:1723233/1‑100 (MQ=255) aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCg > 1:592149/1‑99 (MQ=255) aCCTCTCCTTCCAGGCCACTTTTATTAGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt > 1:1459678/1‑100 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt < 2:369728/100‑1 (MQ=255) ctctCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTa < 2:859208/100‑1 (MQ=255) tctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTaa > 1:39025/1‑100 (MQ=255) ctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTaa > 2:138753/1‑99 (MQ=255) ctcCTTCCAGGCCACTTTTATTCGCCCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAc < 2:2250974/100‑1 (MQ=255) tcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAcc > 1:1230013/1‑100 (MQ=255) cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGCTCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg > 2:362084/1‑100 (MQ=255) cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg > 1:200162/1‑100 (MQ=255) ttCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCgg < 1:964835/100‑1 (MQ=255) tCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGc > 1:141737/1‑100 (MQ=255) ccAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGc < 1:2145868/99‑1 (MQ=255) ccAGGCCACCTTTATTCGCGCCTGCGAAGCAGCAGGGAACACCGCAGAAGCTATACCCCCGCAGCCAGCGCGCATTATCGAACCTGCCGTTCACCCGGCa > 2:2229947/1‑100 (MQ=255) aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCAc < 2:1465745/99‑1 (MQ=255) aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACt < 2:1949652/100‑1 (MQ=255) ggCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTg < 2:1229838/100‑1 (MQ=255) gCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGa < 1:1952791/100‑1 (MQ=255) ccACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGAt > 2:2229222/1‑100 (MQ=255) cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg > 2:951761/1‑100 (MQ=255) cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg > 2:1050893/1‑100 (MQ=255) cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg < 1:2046336/100‑1 (MQ=255) tttATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgcg < 2:1929951/100‑1 (MQ=255) actCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg > 1:2227558/3‑100 (MQ=255) aTTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg > 1:2299933/1‑100 (MQ=255) ttCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGt < 1:2080560/100‑1 (MQ=255) ttCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgc > 2:1805889/1‑95 (MQ=255) tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGt < 1:1043402/99‑1 (MQ=255) tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg < 2:1634280/100‑1 (MQ=255) tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg < 1:2199851/100‑1 (MQ=255) tCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg < 2:67616/100‑1 (MQ=255) cgcgCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa > 2:1953714/1‑100 (MQ=255) cgcCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGaaa < 2:1550674/100‑1 (MQ=255) cGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGTGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCg > 1:1092738/1‑100 (MQ=255) gaagaaGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCgg < 1:1727102/100‑1 (MQ=255) gaagaaGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCgg < 2:1459888/100‑1 (MQ=255) aagaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGa < 1:1203189/100‑1 (MQ=255) agaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt > 1:2381566/1‑100 (MQ=255) agaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt < 1:120476/100‑1 (MQ=255) gaagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg > 1:1498219/1‑100 (MQ=255) agCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGc > 2:164649/1‑100 (MQ=255) cAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCAc > 2:1586383/1‑100 (MQ=255) gggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCg > 1:291197/1‑100 (MQ=255) ggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGt > 2:2350333/1‑100 (MQ=255) gATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCTAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGtt < 2:1529319/100‑1 (MQ=255) aTCAGAGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTg < 2:492453/100‑1 (MQ=255) | ACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTG > NC_000913/2352897‑2353091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |