Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	843,368	(T)6→5	coding (2113/2226 nt)	ybiO ←	mechanosensitive channel protein, intermediate conductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	843,363	0	T	.	100.0%	279.0 / NA	59	coding (2118/2226 nt)	ybiO	mechanosensitive channel protein, intermediate conductance
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/31);  total (28/31)

GGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAA  >  NC_000913/843265‑843458                                                                                                   |                                                                                                ggTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtt                                                                                                >  1:1051736/1‑100 (MQ=255) ggTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGt                                                                                                 >  2:402749/1‑99 (MQ=255)    tCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACAACCGCCAGGTCGAAATGttttt                                                                                             >  1:634834/1‑100 (MQ=255)     cccccGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttc                                                                                            >  1:1482279/1‑99 (MQ=255)      ccccGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttca                                                                                           <  1:1441399/100‑3 (MQ=255)       cccGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttcac                                                                                          >  1:914926/1‑97 (MQ=255)       cccGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttcac                                                                                          >  1:1535182/1‑97 (MQ=255)        ccGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttcacc                                                                                         >  2:700948/1‑96 (MQ=255)         cggcggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCt                                                                                       <  1:1547387/100‑1 (MQ=255)          ggcggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTg                                                                                      >  1:11148/1‑100 (MQ=255)          ggcggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTg                                                                                      >  2:610946/1‑100 (MQ=255)           gcggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTgg                                                                                     <  1:348639/100‑1 (MQ=255)            cggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGtttttcac                                                                                          <  2:1381015/95‑4 (MQ=255)            cggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGc                                                                                    <  1:1038336/100‑1 (MQ=255)             ggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCt                                                                                   >  2:155005/1‑100 (MQ=255)                   ggTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCGGGCTGTCGa                                                                              >  2:1560086/1‑99 (MQ=255)                    gTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAgg                                                                            <  2:11148/100‑1 (MQ=255)                    gTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAgg                                                                            >  1:934561/1‑100 (MQ=255)                    gTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAgg                                                                            >  1:934313/1‑100 (MQ=255)                     ttCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAggg                                                                           <  2:657732/100‑1 (MQ=255)                       cAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACTCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCa                                                                         <  2:861022/100‑1 (MQ=255)                          ccGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAg                                                                      <  1:783192/100‑1 (MQ=255)                          ccGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAg                                                                      <  2:1308591/100‑1 (MQ=255)                            ggggTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCg                                                                    <  1:94364/100‑1 (MQ=255)                                 cgcgCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTAcc                                                                >  1:728995/1‑99 (MQ=255)                                 cgcgCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCg                                                               <  1:1565667/100‑1 (MQ=255)                                 cgcgCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCg                                                               <  1:1498390/100‑1 (MQ=255)                                 cgcgCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCg                                                               >  2:109563/1‑100 (MQ=255)                                   cgcCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTc                                                             >  2:101343/1‑100 (MQ=255)                                   cgcCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTc                                                             >  2:868313/1‑100 (MQ=255)                                    gcCCGGCGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCCGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTcc                                                            >  1:236959/1‑100 (MQ=255)                                    gcCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTcc                                                            <  1:1022811/100‑1 (MQ=255)                                     cccGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCa                                                           >  1:1003788/1‑100 (MQ=255)                                        ggAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTg                                                        <  1:610949/100‑1 (MQ=255)                                           gcaggcagCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGttt                                                     <  2:515992/100‑1 (MQ=255)                                              ggcagCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAg                                                  <  1:479151/100‑1 (MQ=255)                                              ggcagCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAg                                                  >  2:56554/1‑100 (MQ=255)                                               gcagcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGt                                                 <  2:599151/100‑1 (MQ=255)                                               gcagcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGt                                                 <  1:1500506/100‑1 (MQ=255)                                               gcagcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGt                                                 <  2:599158/100‑1 (MQ=255)                                                  gcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGc                                              <  1:101128/100‑1 (MQ=255)                                                  gcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGc                                              <  2:1111943/100‑1 (MQ=255)                                                   caCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCa                                             <  2:1051729/100‑1 (MQ=255)                                                    aCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCa                                             >  1:279057/1‑99 (MQ=255)                                                      cTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCg                                          >  2:714463/1‑100 (MQ=255)                                                      cTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCg                                          <  1:1288167/100‑1 (MQ=255)                                                           aaGTCTGCACTGGCGCGCGAACGCCCGCCAGGGCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCgtggtg                                     <  2:640383/100‑1 (MQ=255)                                                              tCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAAc                                  >  1:1199338/1‑100 (MQ=255)                                                                 gCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGaa                               >  1:189437/1‑100 (MQ=255)                                                                 gCACTGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGaa                               <  1:402750/100‑1 (MQ=255)                                                                     tGGCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAacac                           >  1:26307/1‑100 (MQ=255)                                                                      ggCGCGCGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACg                          >  1:375913/1‑100 (MQ=255)                                                                       gcgcgcGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGc                         <  1:109563/100‑1 (MQ=255)                                                                       gcgcgcGAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGc                         <  2:1388139/100‑1 (MQ=255)                                                                           gcgAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCAGTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAgtg                     <  2:842448/100‑1 (MQ=255)                                                                           gcgAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAgtg                     >  1:360330/1‑100 (MQ=255)                                                                           gcgAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAgtg                     <  2:88660/100‑1 (MQ=255)                                                                           gcgAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCCGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAgtg                     <  2:1056727/100‑1 (MQ=255)                                                                            cgAACGCCCGCCAGGTCAAAATG‑TTATTGACATGTCTGGCGAGGGCACAGCGGACCGAGCACAGTGCGAGTGGCAACGGGGCGAACGAAACACGCAgtgt                    >  2:1285448/1‑100 (MQ=255)                                                                             gAACGCCCGCCAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTa                   <  2:37183/100‑1 (MQ=255)                                                                                 gcccgccAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAAcg               >  2:675000/1‑100 (MQ=255)                                                                                  cccgccAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAAcgc              >  1:1248649/1‑100 (MQ=255)                                                                                  cccgccAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAAcgc              >  1:889885/1‑100 (MQ=255)                                                                                    cgccAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCgg            >  2:1460772/1‑100 (MQ=255)                                                                                     gccAGGTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGt           <  1:1119401/100‑1 (MQ=255)                                                                                         ggTCGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTg       >  1:104798/1‑100 (MQ=255)                                                                                            cGAAATG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCtt    >  2:279665/1‑100 (MQ=255)                                                                                              aaaTG‑TTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTaa  >  1:1545909/1‑100 (MQ=255)                                                                                                   |                                                                                                GGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAA  >  NC_000913/843265‑843458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑