Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,212,319 (T)5→4 intergenic (‑316/+384) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,3150T.96.7% 263.9 / 2.0 60intergenic (‑312/+388)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (27/31);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

TAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAA  >  NC_000913/1212218‑1212410
                                                                                                 |                                                                                               
tAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttt                                                                                               <  2:574138/100‑1 (MQ=255)
tAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttt                                                                                               <  1:1519604/100‑1 (MQ=255)
 aGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCtttt                                                                                              >  1:584968/1‑100 (MQ=255)
   cTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttttat                                                                                            <  2:720043/100‑3 (MQ=255)
      taATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTtatata                                                                                        >  1:380106/1‑100 (MQ=255)
       aaTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTtatata                                                                                        <  1:1348065/100‑1 (MQ=255)
       aaTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATaa                                                                                       >  1:737641/1‑100 (MQ=255)
        aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAAc                                                                                      <  2:651080/100‑1 (MQ=255)
         tGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACg                                                                                     <  2:878848/100‑1 (MQ=255)
              taCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGataat                                                                                >  1:900828/1‑100 (MQ=255)
              taCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGataat                                                                                >  1:1166817/1‑100 (MQ=255)
                cATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGAtaataa                                                                              >  2:1108249/1‑100 (MQ=255)
                  ttCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTACCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATg                                                                            <  2:868310/100‑1 (MQ=255)
                   tCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGa                                                                           >  2:1149837/1‑100 (MQ=255)
                   tCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGa                                                                           <  2:170692/100‑1 (MQ=255)
                   tCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGa                                                                           <  2:153483/100‑1 (MQ=255)
                    cAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGaa                                                                          >  1:379779/1‑100 (MQ=255)
                     aaaTGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAat                                                                         >  1:210601/1‑100 (MQ=255)
                     aaaTGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAat                                                                         <  2:92216/100‑1 (MQ=255)
                      aaTGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAata                                                                        >  2:269833/1‑100 (MQ=255)
                         gACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATaa                                                                     >  1:142180/1‑100 (MQ=255)
                         gACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATaa                                                                     <  1:1185809/100‑1 (MQ=255)
                            tGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGca                                                                  <  2:379778/100‑1 (MQ=255)
                               aCATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGcatga                                                               <  2:1518426/100‑1 (MQ=255)
                                cATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGcatgac                                                              >  2:347021/1‑100 (MQ=255)
                                     tGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATgag                                                         <  2:218531/100‑1 (MQ=255)
                                         aTTACAACATAGCCTTTATGCTACTTTGTGCCTATTTGCTAAACATTATAGTTGCCTTTTATATAACGATAATAATGAATATAAGCATCACATCAGAATa                                                     >  2:1063302/1‑100 (MQ=255)
                                         aTTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATa                                                     >  2:1027352/1‑100 (MQ=255)
                                         aTTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATa                                                     >  2:431140/1‑100 (MQ=255)
                                          ttAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATaa                                                    <  1:679788/100‑1 (MQ=255)
                                           tAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAg                                                   >  2:27099/1‑100 (MQ=255)
                                           tAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAAAATTATGGTTGCCTTTTATAGAACGATAATAATGAATATAAGCATGACATGAGAATAAg                                                   >  2:859148/1‑100 (MQ=255)
                                            aaaaCATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAgg                                                  <  2:1308673/100‑1 (MQ=255)
                                              aaCATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGtt                                                <  1:1526493/100‑1 (MQ=255)
                                              aaCATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGtt                                                >  2:445845/1‑100 (MQ=255)
                                               aCATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGttt                                               >  1:274209/1‑100 (MQ=255)
                                                cATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTc                                              >  2:1205522/1‑100 (MQ=255)
                                                   aGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAAt                                           >  2:625264/1‑100 (MQ=255)
                                                     ccTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAAttt                                         >  2:1108608/1‑100 (MQ=255)
                                                     ccTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAAttt                                         >  1:7420/1‑100 (MQ=255)
                                                        ttATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTg                                      <  1:1027359/100‑1 (MQ=255)
                                                        ttATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTg                                      <  1:411225/100‑1 (MQ=255)
                                                        ttATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTg                                      <  1:1553055/100‑1 (MQ=255)
                                                          aTGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAg                                    <  1:27099/100‑1 (MQ=255)
                                                               aCTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATTACATGAGAATAAGGTTTCAATTTTTGAGTta                                 <  1:543288/98‑1 (MQ=255)
                                                                 tttGTGCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTtatata                             <  1:388677/100‑1 (MQ=255)
                                                                  ttGTGCCAATTTGGTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAg                            <  1:53581/100‑1 (MQ=255)
                                                                   tgtgCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGTCATGAGAATAAGGTTTCAATTTTTGAGTTATATAgg                           <  1:1108615/100‑1 (MQ=255)
                                                                     tgCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGaa                         >  1:1313756/1‑100 (MQ=255)
                                                                      gCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAAt                        >  1:364617/1‑100 (MQ=255)
                                                                      gCCAATTTGCTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAAt                        >  2:48330/1‑100 (MQ=255)
                                                                               cTAAACATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAAcc               <  2:987299/100‑1 (MQ=255)
                                                                                 aaaCATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCt              >  1:132429/1‑100 (MQ=255)
                                                                                 aaaCATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTg             <  2:1514791/100‑1 (MQ=255)
                                                                                 aaaCATTATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTg             >  2:1482474/1‑100 (MQ=255)
                                                                                      ttATGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCt        <  1:705507/100‑1 (MQ=255)
                                                                                       tATGGTTGCCTTTTATATAACGATAATAATGCATCTAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTg       <  2:1311479/100‑1 (MQ=255)
                                                                                        aTGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTgg      <  2:852428/100‑1 (MQ=255)
                                                                                         tGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGc     <  2:274208/100‑1 (MQ=255)
                                                                                         tGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGc     <  2:737422/100‑1 (MQ=255)
                                                                                         tGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGc     <  2:1214169/100‑1 (MQ=255)
                                                                                         tGGTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGc     <  1:945887/100‑1 (MQ=255)
                                                                                           gTTGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTa   <  1:1108256/100‑1 (MQ=255)
                                                                                            ttGCCTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTaa  >  2:446676/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAA  >  NC_000913/1212218‑1212410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: