| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 497,139 | (T)5→6 | intergenic (+145/‑36) | htpG → / → adk | protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 497,134 | 1 | . | T | 97.6% | 141.8 / ‑3.3 | 42 | intergenic (+140/‑41) | htpG/adk | protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (18/23); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑TTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCA > NC_000913/497035‑497227 | tttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGc < 1:523701/100‑1 (MQ=255) catcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑tttttt < 1:864777/100‑2 (MQ=255) atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑ttttttc < 1:1331196/100‑3 (MQ=255) tcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGGAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑ttttttca > 2:1058305/1‑97 (MQ=255) tcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑ttttttca > 1:696748/1‑97 (MQ=255) tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑ttttttcaaa > 2:1001585/1‑95 (MQ=255) tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑ttttttcaaa > 2:1001581/1‑95 (MQ=255) gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCaaaa < 1:1529838/100‑1 (MQ=255) aCTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCaaaaaa < 2:783673/100‑1 (MQ=255) tttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAAtt > 2:881511/1‑100 (MQ=255) tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCg < 2:593972/100‑1 (MQ=255) cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTAAAAAAATTCGac > 2:697636/1‑100 (MQ=255) cAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGacac > 2:237263/1‑100 (MQ=255) aaaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGAcaca < 1:8756/100‑1 (MQ=255) ttATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACAttt > 1:87089/1‑100 (MQ=255) tcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAgg < 2:325349/100‑1 (MQ=255) cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAggg > 2:696008/1‑100 (MQ=255) ccATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGa > 2:509864/1‑100 (MQ=255) aTTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtt > 2:1296406/1‑100 (MQ=255) ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc < 1:1444822/100‑1 (MQ=255) ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc < 2:1506261/100‑1 (MQ=255) tttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAAt < 1:881518/100‑1 (MQ=255) ttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATg < 1:983826/100‑1 (MQ=255) ttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATg < 1:999452/100‑1 (MQ=255) cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg > 2:1527089/1‑100 (MQ=255) cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc < 1:785203/100‑1 (MQ=255) cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc < 1:592207/100‑1 (MQ=255) ggTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTc < 2:341305/100‑1 (MQ=255) ggTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTc < 1:1238025/100‑1 (MQ=255) gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGc < 1:1117997/100‑1 (MQ=255) ccTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCt < 2:492855/100‑1 (MQ=255) cTTCCTTGAGGCAATCGCCCGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATCTTCGCAATGTGTATCATTCTGCtt > 2:213251/1‑100 (MQ=255) tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTg < 2:214762/100‑1 (MQ=255) ttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTgg > 2:123445/1‑100 (MQ=255) tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc < 1:509867/100‑1 (MQ=255) cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg > 2:573261/1‑100 (MQ=255) gAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAACATTCTGCTTGTCGCTc > 2:1026886/1‑100 (MQ=255) gAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATATTAAGGGGATTTTCGCAATGAGTATCATTCTGCTTGgcgc > 2:1507032/1‑98 (MQ=255) ggCAATCGCCTGTTGGTGGTATCGTTTATAAAA‑AAAAAAAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg < 2:1553780/100‑1 (MQ=255) ggCAATCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGTCACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg > 2:1562538/1‑100 (MQ=255) aTCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg > 2:420246/1‑100 (MQ=255) tCGCCTGTTGGTGGTATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcgc > 2:341042/1‑100 (MQ=255) gtggtATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg < 1:819263/100‑1 (MQ=255) gtggtATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg < 1:237264/100‑1 (MQ=255) ggtATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc < 2:202105/100‑1 (MQ=255) gtATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAATGGGATTTTCGCAATGCTTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACt < 1:111352/100‑1 (MQ=255) tATCGTTTAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTc < 2:1360595/100‑1 (MQ=255) ntttAT‑CGCTTTTTTCATAAAATTCGACACATTTTTAGGNGATTTTNGCAAGGNGTATNATTCTGCTTGGCGCTCCNGNCGNGGGNAAAGGGTCNCAGGc > 2:618690/2‑100 (MQ=255) tAT‑CGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa > 1:418587/1‑100 (MQ=255) | TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAT‑CGC‑TTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCA > NC_000913/497035‑497227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |