Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 710,882 | +T | intergenic (‑157/+53) | uof ← / ← fldA | ryhB‑regulated fur leader peptide/flavodoxin 1 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 710,878 | 1 | . | T | 92.3% | 214.4 / 11.5 | 65 | intergenic (‑153/+57) | uof/fldA | ryhB‑regulated fur leader peptide/flavodoxin 1 |
Reads supporting (aligned to +/- strand): ref base . (3/2); new base T (29/31); total (32/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 |
ATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTC > NC_000913/710779‑710970 | ataaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAatta > 2:1547617/1‑100 (MQ=255) aaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑tt‑ttt < 1:930272/100‑2 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑tt‑tttg < 2:1400820/100‑3 (MQ=255) gTGGCATAGCTTACAGTGAGTACGGCGAGGTGACTTGAACATCTGGATATTGTACAGGTACAACAGCAAAAGTCACAAATTTGCAGCAATTA‑gt‑tgt < 2:1313704/97‑3 (MQ=255) ttgCACAGCTTACATTTACAACGGCAATGCCACTTGAACACCGTGACATTGTACAGGTACTACTGCAAAAGTTACAAAGTTGTAGCAATTA‑tt‑tttgat < 2:458258/97‑5 (MQ=255) tGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑tt‑tttgatt < 1:1408666/100‑6 (MQ=255) ggCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGAAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTg > 1:395835/1‑100 (MQ=255) gCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTgg > 1:996960/1‑100 (MQ=255) gCACACCTTACATTTACAACAGCAAGGCTACGTAAACACAGGGACATTGTACAGGTACAACAGCAAAAGGTACAAATTTGTAGCAATTA‑tt‑tttgatt < 2:1538847/98‑6 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCAtt < 2:729535/100‑1 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTa > 1:1152743/1‑100 (MQ=255) ttACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATc < 1:367005/100‑1 (MQ=255) cATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTAt > 1:1119804/1‑100 (MQ=255) aTTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTAtt < 1:340082/100‑1 (MQ=255) tttACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTa < 2:468227/100‑1 (MQ=255) tttACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTa < 1:142912/100‑1 (MQ=255) ttACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGTTACAACAGCATAAGTGACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTaa < 2:228254/100‑1 (MQ=255) tACAACGGCAAGGCCACTTGAACTCCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAAt > 2:1520956/1‑100 (MQ=255) tACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAAt > 1:1361166/1‑100 (MQ=255) cAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATAc > 2:1236592/1‑100 (MQ=255) aCGGCAAGGCCACTTGAACACCGGGACATTGTACAGTTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACgg < 2:279783/100‑1 (MQ=255) aCGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACgg < 1:549988/100‑1 (MQ=255) ggCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCg < 2:31531/100‑1 (MQ=255) ggCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCg < 2:3821/100‑1 (MQ=255) aaGGCCACTTGAACTCCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAg < 1:1520966/100‑1 (MQ=255) aGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGa > 2:1379231/1‑100 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGAc < 2:820298/100‑1 (MQ=255) ccACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATg < 1:1300132/100‑1 (MQ=255) cACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATg < 2:618941/100‑1 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGa > 1:1093515/1‑100 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAg < 1:49594/100‑1 (MQ=255) cTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAg < 1:829366/100‑1 (MQ=255) cTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAg < 1:1420797/100‑1 (MQ=255) cTTGAACACCGGGACATTGTACAGGCACAACAGCAAACGTTACCAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACCGCGTAGACATg > 1:519663/1‑98 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTc < 2:1119797/100‑1 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTc > 1:1498501/1‑100 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTc > 2:1108296/1‑100 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTc > 1:877430/1‑100 (MQ=255) cacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTAcg > 2:1475364/1‑100 (MQ=255) acCGGGACATTGTACGGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTAcgc < 1:1547627/100‑1 (MQ=255) ccGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTAcgcc > 2:597800/1‑100 (MQ=255) gggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTAcgc > 2:1139567/1‑97 (MQ=255) gggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGca > 2:922145/1‑100 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCcaca < 1:1355926/100‑1 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGca < 1:1424996/100‑1 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcat > 2:901597/1‑100 (MQ=255) gACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcat > 2:1208734/1‑100 (MQ=255) ttGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcatcaca < 1:1121675/100‑1 (MQ=255) ttGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcatcaca > 2:211130/1‑100 (MQ=255) gTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcatcacatc > 2:1341730/1‑100 (MQ=255) tACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcatcacatc > 2:209571/1‑99 (MQ=255) naGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAgg > 2:690683/2‑100 (MQ=255) ggTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCa < 1:1130314/100‑1 (MQ=255) gTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCAt > 2:184117/1‑100 (MQ=255) gTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TTATTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCAt > 2:843094/1‑100 (MQ=255) tACAACAGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATCCGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCAt > 1:356244/1‑99 (MQ=255) acaacaGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTg < 2:996952/100‑1 (MQ=255) acaacaGAAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTg < 2:961510/100‑1 (MQ=255) caacaGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTga > 1:1029826/1‑100 (MQ=255) aacaGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTgag < 2:1404184/100‑1 (MQ=255) aGCAAAAGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAAt > 2:295524/1‑100 (MQ=255) aaaGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcg < 1:568318/100‑1 (MQ=255) aaaGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcg < 1:778553/100‑1 (MQ=255) aaaGTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcg < 2:1014628/100‑1 (MQ=255) gTTACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcgtcg > 2:3829/1‑100 (MQ=255) tACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCgag < 2:1498491/100‑1 (MQ=255) tACAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCgag < 1:843100/100‑1 (MQ=255) cAAATTTGTAGCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGAt < 1:716029/100‑1 (MQ=255) aaTATGTAGCAATTATTT‑TTGAATGGCATTAACTATTAATTCGGCGTATATATGAGTCTATGCCTCATCACATCAGGCATTGAGAATTTCGTCGAGATGc > 2:1110153/1‑100 (MQ=255) gCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc < 1:1522626/100‑1 (MQ=255) gCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc > 2:411163/1‑100 (MQ=255) gCAATTATTT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc > 1:1363653/1‑100 (MQ=255) | ATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTA‑TT‑TTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTC > NC_000913/710779‑710970 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |