Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,665,195 (T)7→8 intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,665,1881.T95.3% 142.1 / ‑0.1 43intergenic (+68/‑127)dmsD/clcBtwin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (22/19);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATC  >  NC_000913/1665093‑1665285
                                                                                                 |                                                                                                 
tGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttt                                                                                               <  2:920741/100‑1 (MQ=255)
   cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATATGTTACAGTGTCACGttttttt                                                                                            <  2:1209978/100‑1 (MQ=255)
   cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                            >  2:215701/1‑100 (MQ=255)
   cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                            <  2:327479/100‑1 (MQ=255)
   cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                            <  2:104920/100‑1 (MQ=255)
      ggTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttttat                                                                                         >  1:1121984/1‑97 (MQ=255)
       gTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttttatc                                                                                        >  1:1081556/1‑96 (MQ=255)
       gTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttttatc                                                                                        >  1:297322/1‑96 (MQ=255)
       gTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttttatc                                                                                        <  2:734773/100‑5 (MQ=255)
         tAAACCGCTGTTTCGTTAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTAtctc                                                                                       <  1:1250282/100‑1 (MQ=255)
         tAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTAtctc                                                                                       <  1:1111697/100‑1 (MQ=255)
         tAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttttttttatct                                                                                      >  2:849878/1‑94 (MQ=255)
          aaaCCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATctct                                                                                      <  1:1111875/100‑1 (MQ=255)
             ccGCTGTTTCGATAGGATCACCGTATTATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCtt                                                                                     <  2:553688/98‑1 (MQ=255)
              cGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTaaa                                                                                  >  2:435768/1‑100 (MQ=255)
                 tGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgca                                                                               <  1:1086646/100‑1 (MQ=255)
                  gTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcac                                                                              >  1:883227/1‑100 (MQ=255)
                   tttCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacg                                                                             >  2:1396535/1‑100 (MQ=255)
                           ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                     >  1:329255/1‑100 (MQ=255)
                                aCCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCg                                                                <  1:1185331/100‑1 (MQ=255)
                                    tAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                            >  1:1428968/1‑100 (MQ=255)
                                       tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                         <  2:1183265/100‑1 (MQ=255)
                                        atTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCct                                                        >  2:91552/1‑100 (MQ=255)
                                        atTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCct                                                        >  2:1465126/1‑100 (MQ=255)
                                         ttGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctc                                                       <  1:435770/100‑1 (MQ=255)
                                             cGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtttt                                                   >  2:1519591/1‑100 (MQ=255)
                                             cGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt                                                    <  2:607461/99‑1 (MQ=255)
                                              ggATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTg                                                  >  2:470131/1‑100 (MQ=255)
                                                  ggTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGc                                              <  1:1314829/100‑1 (MQ=255)
                                                       cGATTACACGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAAt                                         >  1:827947/1‑100 (MQ=255)
                                                            aCGCGCAATCCGGCAATAATAGGTTACAGTGTCACGGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCg                                    <  2:1249602/100‑1 (MQ=255)
                                                             cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc                                   >  2:596972/1‑100 (MQ=255)
                                                              gcgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGcc                                  >  1:204833/1‑100 (MQ=255)
                                                                   aTCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgtgt                             <  1:560722/100‑1 (MQ=255)
                                                                        gCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAt                        >  2:1444489/1‑100 (MQ=255)
                                                                         cAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAtt                       >  2:236489/1‑100 (MQ=255)
                                                                         cAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAtt                       >  2:836496/1‑100 (MQ=255)
                                                                           ataataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc                     >  1:1093134/1‑100 (MQ=255)
                                                                           ataataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc                     >  1:908461/1‑100 (MQ=255)
                                                                             aataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGccc                   <  2:1011896/100‑1 (MQ=255)
                                                                             aataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGccc                   >  2:220580/1‑100 (MQ=255)
                                                                              ataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCa                  <  1:1396545/100‑1 (MQ=255)
                                                                               taGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTTTTTCATTGCCCAt                 <  2:307436/100‑1 (MQ=255)
                                                                                 ggTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAttt               >  2:690883/1‑100 (MQ=255)
                                                                                 ggTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAttt               >  2:762188/1‑100 (MQ=255)
                                                                                  gTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc              <  2:888358/100‑1 (MQ=255)
                                                                                     aCAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg            <  1:470134/99‑1 (MQ=255)
                                                                                     aCAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc           <  1:91552/100‑1 (MQ=255)
                                                                                     aCAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc           <  2:1495453/100‑1 (MQ=255)
                                                                                      cAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCt          >  2:1225020/1‑100 (MQ=255)
                                                                                       aGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTc         <  2:329253/100‑1 (MQ=255)
                                                                                          gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg      >  2:538447/1‑100 (MQ=255)
                                                                                             aCGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGcttc  >  2:923346/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
TGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATC  >  NC_000913/1665093‑1665285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: