Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T97.1% 239.9 / 0.3 69coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base T (31/36);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

GTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469553‑2469743
                                                                                                 |                                                                                               
gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttt                                                                                             >  1:1186225/1‑100 (MQ=255)
 tttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑tttt                                                                                            <  2:995693/100‑1 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                       <  2:1112199/100‑3 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                       <  1:891620/100‑3 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                       <  2:768559/100‑3 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                       <  1:891605/100‑3 (MQ=255)
        catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttcgt                                                                                     <  1:1260845/100‑5 (MQ=255)
         atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttcgtt                                                                                    <  2:712788/100‑6 (MQ=255)
          ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                    <  1:594789/100‑1 (MQ=255)
            cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  <  2:1489684/100‑1 (MQ=255)
            cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  <  2:1128147/100‑1 (MQ=255)
            cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  >  2:1230993/1‑100 (MQ=255)
             tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 >  1:130720/1‑100 (MQ=255)
             tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 >  2:401627/1‑100 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTCTGCTTTTGCTATTTTTTTTTCGTTGCta                                                                                 >  1:1388225/1‑100 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  1:60093/1‑100 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttttttttt                                                                                 >  2:945037/1‑90 (MQ=255)
                cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              >  2:594406/1‑100 (MQ=255)
                  agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  1:1155931/100‑1 (MQ=255)
                  agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  2:504452/100‑1 (MQ=255)
                  agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  1:340324/1‑100 (MQ=255)
                   gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  2:1046200/100‑1 (MQ=255)
                           aGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGc                                                                   <  1:1344706/100‑1 (MQ=255)
                            gTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCt                                                                  >  1:90747/1‑100 (MQ=255)
                            gTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCt                                                                  >  2:1389785/1‑100 (MQ=255)
                              tataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCTAATAATATCTACTGCTaa                                                                >  2:242788/1‑100 (MQ=255)
                                       ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAtt                                                       <  2:1218249/100‑1 (MQ=255)
                                        cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      >  1:340380/1‑100 (MQ=255)
                                         ttccATTTTTTTTTTATTTATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAtttt                                                      >  2:661770/1‑99 (MQ=255)
                                         ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     >  1:698432/1‑100 (MQ=255)
                                         ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     <  2:280852/100‑1 (MQ=255)
                                         ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     >  2:788202/1‑100 (MQ=255)
                                           ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  2:1157384/100‑1 (MQ=255)
                                           ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  1:641608/100‑1 (MQ=255)
                                            cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                  <  1:1100796/100‑1 (MQ=255)
                                               ttttGGTTTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTa                                                <  1:1367527/99‑1 (MQ=255)
                                                tttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAc                                              <  1:1231000/100‑1 (MQ=255)
                                                 ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:624964/100‑1 (MQ=255)
                                                  tGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTTTTTTTTTTTAATATCTACTGCTAAAAATCAATTTATAtttatc                                             >  2:1145754/1‑94 (MQ=255)
                                                   ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTCAAAATCAATTTATATTAAcccc                                           >  2:1047152/1‑100 (MQ=255)
                                                   ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                           >  1:1138782/1‑100 (MQ=255)
                                                   ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAccc                                            >  2:200999/1‑99 (MQ=255)
                                                     tgtATTCATTAATATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  2:1533801/100‑1 (MQ=255)
                                                     tgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  1:401628/100‑1 (MQ=255)
                                                     tgtATTCATTAACATGCAAAAACAATAAATCTATGCGTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         >  1:1107212/1‑100 (MQ=255)
                                                      gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        >  1:869450/1‑100 (MQ=255)
                                                          tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    <  1:1389795/100‑1 (MQ=255)
                                                             ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgt                                  >  1:755770/1‑99 (MQ=255)
                                                                aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                              <  1:663786/100‑1 (MQ=255)
                                                                 cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             <  2:1170018/100‑1 (MQ=255)
                                                                  aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                            >  1:97541/1‑100 (MQ=255)
                                                                   tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                            >  2:419568/1‑99 (MQ=255)
                                                                   tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  2:130720/100‑1 (MQ=255)
                                                                    gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:543408/1‑100 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTCTAATAATATCTACTGCTAAAAATCAATTTATATTTACCCCACTAATAGTGTATTCat                          >  2:759593/1‑99 (MQ=255)
                                                                      aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        <  2:575346/100‑1 (MQ=255)
                                                                       aaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatt                       <  1:1007383/100‑1 (MQ=255)
                                                                        aaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTATAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatta                      >  2:138767/1‑100 (MQ=255)
                                                                        aaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatta                      <  2:1321177/100‑1 (MQ=255)
                                                                                aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt              >  1:1543268/1‑100 (MQ=255)
                                                                                 ttccgatcTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             <  2:1549183/93‑1 (MQ=255)
                                                                                 aTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             >  1:1167903/1‑100 (MQ=255)
                                                                                 aTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             <  2:692504/100‑1 (MQ=255)
                                                                                  tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:171886/100‑1 (MQ=255)
                                                                                    tATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGa          <  1:338021/100‑1 (MQ=255)
                                                                                     aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAACAGTGTATTCATATTATATTTTTTTTGAt         >  1:1115240/1‑100 (MQ=255)
                                                                                       gCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg       <  2:1432182/100‑1 (MQ=255)
                                                                                       gCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      >  2:1170591/1‑100 (MQ=255)
                                                                                       gCTTTTGATATTTTTTTTCGTTGCTCATCATATCTACTGCTAAAATTCACTTTCTATTCACCCCACCAACAGTGTATTAATATTATATTTTTTTTGATAg       <  2:1312935/100‑1 (MQ=255)
                                                                                        cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      <  1:25184/100‑1 (MQ=255)
                                                                                        cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      <  1:27490/100‑1 (MQ=255)
                                                                                        cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      <  2:60093/100‑1 (MQ=255)
                                                                                         ttttGTTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg       <  2:1000692/98‑1 (MQ=255)
                                                                                         ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        >  2:118447/1‑97 (MQ=255)
                                                                                          tttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTAGAtcggaa    >  1:1549193/1‑95 (MQ=255)
                                                                                           ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATACTAACCCCACGAATAGCGTATTCATCTTATATTTTTTTTTATAGacac   >  2:1061284/1‑100 (MQ=255)
                                                                                            tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCTCTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  <  1:594409/100‑1 (MQ=255)
                                                                                            tGCTATTTTTTTTCGTTGCTAATAATATCTAATAATAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  <  2:1516863/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469553‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: