Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,563,518 (A)5→6 intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,5131.A94.4% 182.6 / 4.2 54intergenic (+396/‑79)yffP/yffQCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base A (24/27);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC  >  NC_000913/2563415‑2563606
                                                                                                   |                                                                                              
ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTa                                                                                               >  1:1037279/1‑100 (MQ=255)
ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTa                                                                                               >  2:94325/1‑100 (MQ=255)
ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTa                                                                                               >  1:696553/1‑100 (MQ=255)
 gTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaa                                                                                              >  2:906641/1‑100 (MQ=255)
   gAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTACCAACAACAAAATAAAGATGGGAGGTaa                                                                                              >  2:974294/1‑98 (MQ=255)
     aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaa                                                                                         >  1:1329330/1‑99 (MQ=255)
       tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                       >  2:29696/1‑97 (MQ=255)
                    tCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAg                                                                           <  2:1422584/100‑1 (MQ=255)
                      ncTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  2:407630/2‑100 (MQ=255)
                      gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  1:6838/1‑100 (MQ=255)
                      gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGCGGGAAGt                                                                          >  1:224434/1‑99 (MQ=255)
                        tttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGc                                                                       <  2:386394/100‑1 (MQ=255)
                         ttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGcn                                                                      <  1:85941/100‑2 (MQ=255)
                           ttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCttt                                                                    <  1:1189206/100‑1 (MQ=255)
                                 tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGt                                                              >  1:206914/1‑100 (MQ=255)
                                  cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                             <  1:860617/100‑1 (MQ=255)
                                   aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAAGAACAAAATAAAGATGGGAGGTAAAAAATGAAGCGGTGGGAAGTGGCTTTACTGGTGa                                                            >  2:729304/1‑100 (MQ=255)
                                   aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                             >  1:606701/1‑99 (MQ=255)
                                   aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGa                                                            >  2:1271101/1‑100 (MQ=255)
                                     gCATTTTTGTTTTTTTTTATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGAt                                                           >  2:1438442/1‑98 (MQ=255)
                                     gCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTTGTGATg                                                          <  1:906649/100‑1 (MQ=255)
                                      cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg                                                         <  2:1037272/100‑1 (MQ=255)
                                      cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg                                                         <  1:176454/100‑1 (MQ=255)
                                         ttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTaa                                                      <  2:1024146/100‑1 (MQ=255)
                                           ttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAACATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATg                                                    <  1:529145/100‑1 (MQ=255)
                                             gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt                                                  >  1:254001/1‑100 (MQ=255)
                                              tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg                                                 <  1:205972/100‑1 (MQ=255)
                                                  aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                             >  1:518713/1‑100 (MQ=255)
                                                    tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                           <  2:1447646/100‑1 (MQ=255)
                                                      aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         <  2:948562/100‑1 (MQ=255)
                                                         caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTa                                       <  1:250610/99‑1 (MQ=255)
                                                         caaCGAAAGGCAGTTCCCAACAACAAAATAAAGAAGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                      >  2:1085705/1‑100 (MQ=255)
                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                     >  2:788114/1‑100 (MQ=255)
                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                     >  2:506845/1‑100 (MQ=255)
                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                     <  1:605283/100‑1 (MQ=255)
                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg                                    >  2:743359/1‑100 (MQ=255)
                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg                                    <  2:1363575/100‑1 (MQ=255)
                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg                                    >  1:421850/1‑100 (MQ=255)
                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg                                    <  2:405855/100‑1 (MQ=255)
                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa                                   >  1:821865/1‑100 (MQ=255)
                                                              aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                 <  2:48932/100‑1 (MQ=255)
                                                                 ggCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgt                              <  1:133956/100‑1 (MQ=255)
                                                                  gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             <  2:606698/100‑1 (MQ=255)
                                                                  gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             <  1:94325/100‑1 (MQ=255)
                                                                   cAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGa                            <  2:720959/100‑1 (MQ=255)
                                                                     gTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAACCAGTGGGAAGTGGCTTTACTGGTGAAGGTAATGGTGGTTTGCTGTATTGAACTGTGAag                          >  2:344086/1‑100 (MQ=255)
                                                                       tCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagga                        <  1:1568201/100‑1 (MQ=255)
                                                                        cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGCAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg                         >  1:406004/1‑98 (MQ=255)
                                                                        cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                       <  1:1265120/100‑1 (MQ=255)
                                                                          caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga                     >  2:466341/1‑100 (MQ=255)
                                                                             caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                  >  2:1535904/1‑100 (MQ=255)
                                                                               acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa                >  2:643970/1‑100 (MQ=255)
                                                                                caaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt               >  1:1268961/1‑100 (MQ=255)
                                                                                caaAATAAAGATGGGAGGTAAACAATGAAACGGTGGGAAGTGGCTCTACTGGTGATGGTAATGTTGGTTTGCTGCATTGAACGGTGAAGGAGGACGCCAt               >  2:1089772/1‑100 (MQ=255)
                                                                                  aaaTAAAGATGGGAGGTAAAAAATGAAACGGAGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa             >  2:569888/1‑100 (MQ=255)
                                                                                      aaaGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt         >  1:1240663/1‑100 (MQ=255)
                                                                                         gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt      <  1:870058/100‑1 (MQ=255)
                                                                                          aTGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt       <  2:1326750/98‑1 (MQ=255)
                                                                                            gggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt   >  1:819963/1‑100 (MQ=255)
                                                                                            gggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt   <  1:1002350/100‑1 (MQ=255)
                                                                                             ggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc  >  2:1200608/1‑100 (MQ=255)
                                                                                                   |                                                                                              
GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC  >  NC_000913/2563415‑2563606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: