Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,563,518 | (A)5→6 | intergenic (+401/‑74) | yffP → / → yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,563,513 | 1 | . | A | 94.4% | 182.6 / 4.2 | 54 | intergenic (+396/‑79) | yffP/yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base . (3/0); new base A (24/27); total (27/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 |
GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC > NC_000913/2563415‑2563606 | ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a > 1:1037279/1‑100 (MQ=255) ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a > 2:94325/1‑100 (MQ=255) ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a > 1:696553/1‑100 (MQ=255) gTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aa > 2:906641/1‑100 (MQ=255) gAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTACCAACAACAAAATAAAGATGGGAGGT‑aa > 2:974294/1‑98 (MQ=255) aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa‑aaa > 1:1329330/1‑99 (MQ=255) tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa‑aaatg > 2:29696/1‑97 (MQ=255) tCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAg < 2:1422584/100‑1 (MQ=255) ncTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTg > 2:407630/2‑100 (MQ=255) gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTg > 1:6838/1‑100 (MQ=255) gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGCGGGAAGt > 1:224434/1‑99 (MQ=255) tttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGc < 2:386394/100‑1 (MQ=255) ttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGcn < 1:85941/100‑2 (MQ=255) ttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCttt < 1:1189206/100‑1 (MQ=255) tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGt > 1:206914/1‑100 (MQ=255) cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTg < 1:860617/100‑1 (MQ=255) aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAAGAACAAAATAAAGATGGGAGGTAAAA‑AATGAAGCGGTGGGAAGTGGCTTTACTGGTGa > 2:729304/1‑100 (MQ=255) aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTg > 1:606701/1‑99 (MQ=255) aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGa > 2:1271101/1‑100 (MQ=255) gCATTTTTGTTTTTTTTTATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGAt > 2:1438442/1‑98 (MQ=255) gCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTTGTGATg < 1:906649/100‑1 (MQ=255) cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg < 2:1037272/100‑1 (MQ=255) cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg < 1:176454/100‑1 (MQ=255) ttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTaa < 2:1024146/100‑1 (MQ=255) ttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAACATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATg < 1:529145/100‑1 (MQ=255) gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt > 1:254001/1‑100 (MQ=255) tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg < 1:205972/100‑1 (MQ=255) aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt > 1:518713/1‑100 (MQ=255) tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc < 2:1447646/100‑1 (MQ=255) aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg < 2:948562/100‑1 (MQ=255) caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTa < 1:250610/99‑1 (MQ=255) caaCGAAAGGCAGTTCCCAACAACAAAATAAAGAAGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt > 2:1085705/1‑100 (MQ=255) aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt > 2:788114/1‑100 (MQ=255) aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt > 2:506845/1‑100 (MQ=255) aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt < 1:605283/100‑1 (MQ=255) aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg > 2:743359/1‑100 (MQ=255) aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg < 2:1363575/100‑1 (MQ=255) aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg > 1:421850/1‑100 (MQ=255) aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg < 2:405855/100‑1 (MQ=255) aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa > 1:821865/1‑100 (MQ=255) aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc < 2:48932/100‑1 (MQ=255) ggCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgt < 1:133956/100‑1 (MQ=255) gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg < 2:606698/100‑1 (MQ=255) gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg < 1:94325/100‑1 (MQ=255) cAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGa < 2:720959/100‑1 (MQ=255) gTTCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAACCAGTGGGAAGTGGCTTTACTGGTGAAGGTAATGGTGGTTTGCTGTATTGAACTGTGAag > 2:344086/1‑100 (MQ=255) tCCCAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagga < 1:1568201/100‑1 (MQ=255) cccAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGCAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg > 1:406004/1‑98 (MQ=255) cccAACAACAAAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag < 1:1265120/100‑1 (MQ=255) caacaacaaAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga > 2:466341/1‑100 (MQ=255) caacaaAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc > 2:1535904/1‑100 (MQ=255) acaaAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 2:643970/1‑100 (MQ=255) caaAATAAAGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt > 1:1268961/1‑100 (MQ=255) caaAATAAAGATGGGAGGT‑AAACAATGAAACGGTGGGAAGTGGCTCTACTGGTGATGGTAATGTTGGTTTGCTGCATTGAACGGTGAAGGAGGACGCCAt > 2:1089772/1‑100 (MQ=255) aaaTAAAGATGGGAGGTAAAA‑AATGAAACGGAGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa > 2:569888/1‑100 (MQ=255) aaaGATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt > 1:1240663/1‑100 (MQ=255) gATGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt < 1:870058/100‑1 (MQ=255) aTGGGAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt < 2:1326750/98‑1 (MQ=255) gggAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt > 1:819963/1‑100 (MQ=255) gggAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt < 1:1002350/100‑1 (MQ=255) ggAGGTAAAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc > 2:1200608/1‑100 (MQ=255) | GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAA‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC > NC_000913/2563415‑2563606 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |