Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,242,942	Δ66 bp	coding (510‑575/891 nt)	malG ←	maltose transporter subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4242942	4243007	66	49 [0]	[1] 49	malG	maltose transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4242941	0 (0.000)	44 (0.740)	38/184	0.8	98.9%	coding (576/891 nt)	malG	maltose transporter subunit
	?	NC_000913	4243008 =	1 (0.020)					coding (509/891 nt)	malG	maltose transporter subunit

CCTGCCACGGTGTCGCACCATCCAGCGCAGCAGCTTCTTCCAGCGAACTGTCGATGGTTTCGAAATAGCCTTTGATGGTCCAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCA  >  NC_000913/4242913‑4243107                                                                                                |                                                                                                    taaacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATccc                                                                                                 <  1:1460896‑M2/5‑1 (MQ=255)  aaacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATccca                                                                                                >  1:97200‑M2/95‑100 (MQ=255)  aaacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATccca                                                                                                >  1:97205‑M2/95‑100 (MQ=255)   aacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggccaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcccac                                                                                               >  2:1034392‑M2/94‑100 (MQ=255)     cgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcccaccc                                                                                             <  2:1235403‑M2/9‑1 (MQ=255)          gaatgaatacaaccgccagaatcggtactgacagcggcaacagaacaagccggaagacctgccacggggtcgaaccatccagcgcaATCCCACCCAGATa                                                                                        <  2:213588‑M2/14‑1 (MQ=255)            atgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATAcg                                                                                      >  2:1563889‑M2/85‑100 (MQ=255)                 taccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGaaa                                                                                 >  1:117298‑M2/80‑100 (MQ=255)                  accaccgccagaatccgtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGaaa                                                                                 <  2:920293‑M2/21‑1 (MQ=255)                    caccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCGCACCCAGATACGCGAAAAtt                                                                              >  2:562763‑M2/77‑100 (MQ=255)                    caccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAtt                                                                              >  1:767128‑M2/77‑100 (MQ=255)                      ccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTAc                                                                            >  1:1538309‑M2/75‑100 (MQ=255)                      ccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTAc                                                                            <  1:804074‑M2/26‑1 (MQ=255)                       cgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTAcg                                                                           <  2:1429297‑M2/27‑1 (MQ=255)                               tcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTg                                                                   <  1:477662‑M2/35‑1 (MQ=255)                                cggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGa                                                                  >  2:187514‑M2/65‑100 (MQ=255)                                     ctgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTAtt                                                             >  1:1504980‑M2/60‑100 (MQ=255)                                      tgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTCTTc                                                            >  2:59147‑M2/59‑100 (MQ=255)                                      tgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTc                                                            <  1:508269‑M2/42‑1 (MQ=255)                                       gacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCa                                                           <  1:1225615‑M2/43‑1 (MQ=255)                                        acagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAg                                                          >  2:362053‑M2/57‑100 (MQ=255)                                        acagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAg                                                          <  2:98115‑M2/44‑1 (MQ=255)                                         cagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAgg                                                         <  1:1439640‑M2/45‑1 (MQ=255)                                               caacaggacaaggcggaaggccttccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatg                                                   <  2:1191574‑M2/51‑1 (MQ=255)                                                aacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatga                                                  >  1:108430‑M2/49‑100 (MQ=255)                                                  caggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGCGTATTCAGGCCaatga                                                  >  2:1450‑M2/47‑98 (MQ=255)                                                   aggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatgaatg                                               <  2:978059‑M2/55‑1 (MQ=255)                                                       caaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATgaatggaat                                           >  1:373121‑M2/42‑100 (MQ=255)                                                       caaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATgaatggaat                                           >  1:373078‑M2/42‑100 (MQ=255)                                                        aaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATgaatggaatg                                          <  2:1452050‑M2/60‑1 (MQ=255)                                                              gaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCa                                    >  1:1213154‑M2/35‑100 (MQ=255)                                                                  gcctgccacggtgtcgcaccatccagcgcgATCCCACCCATATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCa                                <  2:680127‑M2/70‑1 (MQ=255)                                                                   cctgccacggtgtcgcaccatccagcgcaATCCCACCCGGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAg                               >  2:969688‑M2/30‑100 (MQ=255)                                                                   cctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAg                               >  2:1110412‑M2/30‑100 (MQ=255)                                                                    ctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGa                              >  1:1050028‑M2/29‑100 (MQ=255)                                                                    ctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGa                              <  1:250128‑M2/72‑1 (MQ=255)                                                                     tgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGAc                             <  1:1055090‑M2/73‑1 (MQ=255)                                                                       ccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGa                           >  2:591340‑M2/26‑100 (MQ=255)                                                                         tGATGGTCCAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATc                         <  2:411435/100‑1 (MQ=255)                                                                         acggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATc                         <  1:963809‑M2/77‑1 (MQ=255)                                                                         acggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATc                         <  2:1024529‑M2/77‑1 (MQ=255)                                                                             tgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAaac                     <  1:927874‑M2/81‑1 (MQ=255)                                                                                 gcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACg                 >  1:1216369‑M2/16‑100 (MQ=255)                                                                                 gcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACg                 >  1:1431348‑M2/16‑100 (MQ=255)                                                                                    ccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCAt              >  1:1465284‑M2/13‑100 (MQ=255)                                                                                         cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAc         >  1:949141‑M2/8‑100 (MQ=255)                                                                                           gcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAcgc       >  2:774424‑M2/6‑100 (MQ=255)                                                                                            cgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAcgcg      <  2:708600‑M2/96‑1 (MQ=255)                                                                                                aTCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCa  >  1:1139271/1‑100 (MQ=255)                                                                                                |                                                                                                    CCTGCCACGGTGTCGCACCATCCAGCGCAGCAGCTTCTTCCAGCGAACTGTCGATGGTTTCGAAATAGCCTTTGATGGTCCAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCA  >  NC_000913/4242913‑4243107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑