Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,661	0	T	C	20.3%	130.4 / 30.2	64	K505E (AAA→GAA)	clpB	protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base C (7/6);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGC  >  NC_000913/2732564‑2732758                                                                                                  |                                                                                                  gCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGc                                                                                                 >  1:746954/1‑100 (MQ=255) gCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGc                                                                                                 <  1:1092126/100‑1 (MQ=255) gCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGc                                                                                                 <  1:1252120/100‑1 (MQ=255)    aTTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGt                                                                                              <  1:1441791/100‑1 (MQ=255)           cGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAg                                                                                       <  2:470437/100‑1 (MQ=255)              cGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTc                                                                                    <  1:41824/100‑1 (MQ=255)              cGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTc                                                                                    <  1:41544/100‑1 (MQ=255)              cGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTc                                                                                    <  1:1430269/100‑1 (MQ=255)              cGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTc                                                                                    >  1:889358/1‑100 (MQ=255)               ggTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCAAGGTGCTTTACCAGTTCCGGGATTTCGCCGTATTGCAGTTCa                                                                                   >  2:1102678/1‑100 (MQ=255)                gTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAg                                                                                  <  2:1271010/100‑1 (MQ=255)                gTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAg                                                                                  <  2:407980/100‑1 (MQ=255)                  cACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGTTTTTTGCCGGATTGCAGTTCAgcc                                                                                >  2:102703/1‑98 (MQ=255)                   aCTTTATTACGCGACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACa                                                                               >  1:1030751/1‑100 (MQ=255)                    cTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACAt                                                                              >  2:1448498/1‑100 (MQ=255)                        attaCGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCcgc                                                                          >  2:613139/1‑100 (MQ=255)                         ttaCGCAACAGACGCATAGTTTTGCCTTCGTGCTGCGATGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCcgcg                                                                         <  2:1055638/100‑1 (MQ=255)                          taCGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCgcgc                                                                        <  2:1541725/100‑1 (MQ=255)                           aCGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGcc                                                                       >  1:358622/1‑100 (MQ=255)                            cGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGcc                                                                       >  2:1075904/1‑99 (MQ=255)                             gCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAg                                                                     <  1:1090208/100‑1 (MQ=255)                              cAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAgg                                                                    >  2:929821/1‑100 (MQ=255)                              cAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAgg                                                                    >  1:973876/1‑100 (MQ=255)                              cAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAgg                                                                    >  1:1041253/1‑100 (MQ=255)                                 cAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTcc                                                                 >  2:189414/1‑100 (MQ=255)                                  aGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTccc                                                                <  1:760632/100‑1 (MQ=255)                                   gACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTcccc                                                               >  1:61090/1‑100 (MQ=255)                                    aCGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTccccc                                                              <  2:1028258/100‑1 (MQ=255)                                       cATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCcaca                                                           >  1:187931/1‑100 (MQ=255)                                       cATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCcaca                                                           >  1:580891/1‑100 (MQ=255)                                        aTAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCacac                                                          >  1:1208106/1‑100 (MQ=255)                                             tttGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGa                                                     >  1:1400808/1‑100 (MQ=255)                                                gCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGcc                                                  <  1:546094/100‑1 (MQ=255)                                                 ccTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCt                                                 >  1:302338/1‑100 (MQ=255)                                                  cTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACAAACGCGCCAGGTCCCCCACACGGCGAGCCTg                                                >  1:1309286/1‑100 (MQ=255)                                                    tCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGtt                                              <  2:267808/100‑1 (MQ=255)                                                     cGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTc                                             <  2:1041246/100‑1 (MQ=255)                                                     cGAGCGGCGTTGCGGCTTCCTTTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTc                                             <  1:128828/100‑1 (MQ=255)                                                      gAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCa                                            <  2:1499881/100‑1 (MQ=255)                                                      gAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCa                                            <  2:1473056/100‑1 (MQ=255)                                                      gAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCa                                            >  1:77394/1‑100 (MQ=255)                                                      gAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCa                                            <  2:588453/100‑1 (MQ=255)                                                       aGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCaa                                           >  1:53111/1‑100 (MQ=255)                                                               tGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGAtt                                   >  1:1449940/1‑100 (MQ=255)                                                                gCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGAttt                                  >  1:1443000/1‑100 (MQ=255)                                                                 cGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGAtttt                                 >  1:80868/1‑100 (MQ=255)                                                                   gCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCg                               <  1:613142/100‑1 (MQ=255)                                                                   gCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCg                               <  2:746950/100‑1 (MQ=255)                                                                     ttCCAGTTGCTTTTCCAGTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGcc                             <  2:761208/100‑1 (MQ=255)                                                                          gTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCTGTTTAGACATCCGCGCCAGGTGCCCAACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTc                        >  2:1475910/1‑100 (MQ=255)                                                                          gTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACCTCCGCGCCAGGCCCCCCACTTAGCGCGCATGTTCAATTGCGATTTCCGCCTGTTc                        >  2:1476149/1‑100 (MQ=255)                                                                          gTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTc                        >  1:777195/1‑100 (MQ=255)                                                                           ttGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTcc                       <  2:394542/100‑1 (MQ=255)                                                                              cTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGt                    >  1:913352/1‑100 (MQ=255)                                                                              cTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGt                    >  2:140393/1‑100 (MQ=255)                                                                               ttttCCATTTCTTGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGtt                   >  2:249824/1‑100 (MQ=255)                                                                                tttCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTc                  <  2:598697/100‑1 (MQ=255)                                                                                   ccAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGc               <  1:929829/100‑1 (MQ=255)                                                                                    cAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCt              >  1:146440/1‑100 (MQ=255)                                                                                      gTTCCGGGATTTCGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCttt            >  1:431140/1‑100 (MQ=255)                                                                                         ccGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAAt         >  2:712612/1‑100 (MQ=255)                                                                                          cGGGATTTTGCCGTATTGCAGTCCAGACATCCGCGCCGGGTCCCCCAGACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATg        <  2:1060704/100‑1 (MQ=255)                                                                                           gggATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATgg       <  1:800082/100‑1 (MQ=255)                                                                                             gATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTc     <  2:295545/100‑1 (MQ=255)                                                                                               ttttGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGGCTg   <  1:682280/100‑1 (MQ=255)                                                                                                tttGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGTGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGc  <  2:973868/100‑1 (MQ=255)                                                                                                  |                                                                                                  GCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGC  >  NC_000913/2732564‑2732758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑