| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,705,984 | 0 | T | G | 21.2% | 193.2 / 53.9 | 80 | intergenic (‑194/+114) | dppB/dppA | dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor |
| Reads supporting (aligned to +/- strand): ref base T (29/34); new base G (16/1); total (45/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01 | |||||||||||
GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATC > NC_000913/3705887‑3706078 | gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa > 1:411160/1‑100 (MQ=255) gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTCTcc > 2:987141/1‑100 (MQ=255) ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc < 1:1010741/100‑1 (MQ=255) ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc < 1:593242/100‑1 (MQ=37) gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc < 1:106762/100‑1 (MQ=255) ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc > 2:1439212/1‑100 (MQ=255) cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑aa < 1:1211545/100‑1 (MQ=255) cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑aaaa < 1:267284/100‑1 (MQ=255) tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAAt < 1:478482/100‑1 (MQ=255) cccGCAGAGGCAAAGCGCCACCGGGTATAAAGATTGTTGCCACAGTGAGATTTGTTTATGCCGGATGCGGCGTTAACGACTTATCAGGCCTAC‑AACATCg < 1:1101272/98‑1 (MQ=18) cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCg < 1:321228/100‑1 (MQ=255) cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCg < 2:371093/100‑1 (MQ=255) cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTg < 2:887462/100‑1 (MQ=255) cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTg < 1:324218/100‑1 (MQ=255) gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGc < 2:604494/100‑1 (MQ=255) cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCa > 2:232380/1‑100 (MQ=255) cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCa > 2:1527325/1‑100 (MQ=255) gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTc < 1:202366/100‑1 (MQ=255) cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCa > 2:690466/1‑100 (MQ=255) acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGATGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCaa < 1:550814/100‑1 (MQ=255) caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGTCCTAC‑AAAATCGTGCAAATTCAAt > 1:726380/1‑100 (MQ=255) caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt < 1:1279620/100‑1 (MQ=255) caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt > 2:976319/1‑100 (MQ=255) caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt < 2:838362/100‑1 (MQ=255) ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACAtt > 2:1148658/1‑100 (MQ=255) cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTg < 1:226481/100‑1 (MQ=255) cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑g > 2:1485127/1‑100 (MQ=255) ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑At‑tt > 1:1099855/1‑100 (MQ=255) taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑At‑ttt > 1:164209/1‑100 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCg < 2:849645/100‑1 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCg > 1:789106/1‑100 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCg < 1:976327/100‑1 (MQ=255) aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGt > 1:1486182/1‑100 (MQ=255) gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTa > 1:251155/1‑100 (MQ=255) ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAgg < 2:1187436/100‑1 (MQ=255) ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAgg < 2:569334/100‑1 (MQ=255) ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAgg > 1:847838/1‑100 (MQ=255) ttaaCAAAGTGCGCTTTGTTTATGCAGGATTCGTCGTTAACGCCTGATCCGGCCTAC‑AAAATCGTTCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGcc < 2:153546/96‑1 (MQ=17) tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTg > 2:1325421/1‑100 (MQ=255) gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGa > 2:1526449/1‑100 (MQ=255) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGAt > 2:1348016/1‑100 (MQ=255) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATa < 1:294522/100‑1 (MQ=255) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATa > 2:131025/1‑100 (MQ=255) aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATaa > 2:987632/1‑100 (MQ=255) aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGc < 1:273675/100‑1 (MQ=255) gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCg > 2:139166/1‑100 (MQ=255) tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCgg < 2:563755/100‑1 (MQ=255) cTTTGTTTATGGCGGATGCGGCGTTAAAGCCTTATCCGGCCTAG‑AAAAGCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑GTTCGTAGGCCTGATAAGCGGAgcg < 2:1417513/100‑1 (MQ=17) cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAgcg > 1:44386/1‑100 (MQ=255) ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCa < 1:103631/100‑1 (MQ=255) ttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATc > 1:1572164/7‑100 (MQ=18) gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATc > 2:485241/1‑100 (MQ=255) tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCa < 1:232381/100‑1 (MQ=255) tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑‑ATCCCTTGTAGGCCTGATAAGCGTAGCGCATCAg > 1:260919/6‑100 (MQ=18) ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAg > 1:358572/1‑100 (MQ=255) gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GG‑‑AG‑TTATGTAGGCCTGATAAGCGTAGCGCATCAgg > 1:1299694/5‑100 (MQ=21) aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCAGGG‑‑AC‑TAT‑GTAGGCCTGATAAGCATAGCGCATCAGGc > 2:649396/3‑100 (MQ=18) ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAATGTGCAAATTCAATAAATTGCA‑ATTCAA‑CTT‑GTAGGCCTGATAAGCGCAGCGCATCAGGc > 1:1146109/2‑100 (MQ=18) ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TG‑‑AG‑CCACGTAGGCCTGATAAGCGTAGCGCATCAGGc > 1:249526/2‑99 (MQ=17) ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCATGAAAATTCAATACATTGCA‑AG‑‑AT‑TTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCa > 2:1130087/2‑100 (MQ=17) ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCA‑GG‑‑GA‑CTATGTAGGCCTGATAAGCATAGCGCATCAGGCa > 2:461992/2‑100 (MQ=18) gCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt > 1:1317673/1‑100 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CG‑‑AT‑CCACGTAGGCCTGATAAGCGTAGCGCATCAGGCag > 1:993691/1‑99 (MQ=21) ccggataaggcgTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑‑ATCCCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAttt > 1:1230649/9‑100 (MQ=18) ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TG‑‑AG‑CCACGTAGGCCTGATAAGCGTAGCGCATCATGCagt > 1:947137/9‑98 (MQ=17) ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑‑AC‑CAT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt > 1:585039/7‑100 (MQ=18) ggataaggcgTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt > 1:399837/7‑100 (MQ=18) ggATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt < 1:134947/100‑1 (MQ=255) gataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑‑AC‑CAT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt > 1:831241/6‑100 (MQ=18) gataaggcgTTCACGCCGCATCCGGCCTAC‑AAATCCTTGCGAATTCAATATATTGTATGG‑‑AA‑ATTC‑‑AGGCCTGATAAGCGTAGCGCATCAGGCttttcac > 1:320365/6‑97 (MQ=37) aTGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg < 1:345541/100‑1 (MQ=255) taaggcgTTCACGCCGCATCCGACCCAC‑AAAGTCTTGCAAAATCAATAAATTGCAGGA‑‑AC‑GAT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactacgt > 1:1515347/4‑93 (MQ=25) tGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc > 2:1003362/1‑100 (MQ=255) ggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt < 2:505645/100‑1 (MQ=255) gcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATCAGCGGAGCGCATCAGGCCTTttttccttt > 2:131786/1‑100 (MQ=255) cgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg < 1:690469/100‑1 (MQ=255) cgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg < 1:987149/100‑1 (MQ=255) ttAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc > 2:391425/1‑100 (MQ=255) tAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa < 2:1300810/100‑1 (MQ=255) aCGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc < 1:592866/100‑1 (MQ=255) | GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑‑AT‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATC > NC_000913/3705887‑3706078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |