| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 36,231 | T→C | intergenic (‑69/+40) | caiD ← / ← caiC | carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 36,231 | 0 | T | C | 95.2% | 210.4 / ‑1.6 | 63 | intergenic (‑69/+40) | caiD/caiC | carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (28/32); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT > NC_000913/36139‑36327 | ggTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGatta > 1:968940/1‑100 (MQ=255)ggTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGatta > 1:1146477/1‑100 (MQ=255) cAGATGTAATGATTCACTCATTTCTTTTCTCCATTTTTGCTTTTTCATGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGAttatta < 2:1059206/100‑1 (MQ=255) cAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGAttatta < 1:236340/100‑1 (MQ=255) aGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAg < 2:78195/100‑1 (MQ=255) aTGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCt > 2:728797/1‑100 (MQ=255) aTGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCt < 1:170122/100‑1 (MQ=255) tGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTg > 2:334354/1‑100 (MQ=255) gTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTgg > 2:1250596/1‑100 (MQ=255) tAATGATTCACTCATTCCTTTTCTCCATTTTGGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc < 2:114704/100‑1 (MQ=255) aTGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAg < 2:877997/100‑1 (MQ=255) aTTCACTCATTCCTTTTCTCCATTTTTTCTTTTTCTTTGACGACAAAAAAAAAAAAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt < 2:1251588/100‑1 (MQ=255) aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt < 2:1237754/100‑1 (MQ=255) ttCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTa > 2:901625/1‑100 (MQ=255) cTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTc > 1:978946/1‑100 (MQ=255) cTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTc > 2:395111/1‑100 (MQ=255) cATTCCTTTTCTCCATTTTTGCTTTTTCGGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTccc < 2:231591/100‑1 (MQ=255) cATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTccc < 2:47699/100‑1 (MQ=255) ccTTTTCTCCATTTTTTTTTTTTTTTTTACGACAACATCCCTGCAAAAAATATATTTTGTTTTAGAGTGTGAATATTAGCTTTCAGGGTATTTctcttc > 2:995495/1‑97 (MQ=255) ccTTTTCTCCATTTTTTTTTTTTTTTTGACGACAACATCCCTGCAAAAAATGTATATTGTTTTAGAGCGTGATTATTAGCTGTCAGGGTAGTTCCct > 2:434684/1‑97 (MQ=255) tttaCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTATAGAGCGTGATTATAAGCTGGCAGGGTAGTTCCCTGCTGt > 1:97834/1‑100 (MQ=255) ttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGAACTACCctgccagct < 1:825214/100‑6 (MQ=255) tctcCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTc > 2:1416973/1‑100 (MQ=255) ctcCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCa < 2:1515845/100‑1 (MQ=255) ctcCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCa > 1:1037115/1‑100 (MQ=255) tttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCatttattt < 1:933913/100‑1 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc < 2:1037390/100‑1 (MQ=255) tGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCa > 2:227783/1‑100 (MQ=255) cTTTTTCAGGGACGAGAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGa < 1:1550328/100‑1 (MQ=255) cTTTTTCAGGGACGACAACATCCCTGCAAAAACTGCATATTGTTTTAGAGCGTGTTTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAg > 2:65931/1‑99 (MQ=255) cTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGa < 2:513721/100‑1 (MQ=255) tttttCCGGGACAACAACATAAAAGCAAAAAATGTATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAg < 2:869130/98‑1 (MQ=255) tttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttc < 1:895812/100‑1 (MQ=255) tCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctt < 1:395112/100‑1 (MQ=255) aGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttc > 2:1488261/1‑100 (MQ=255) aGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttc > 1:1141054/1‑100 (MQ=255) gggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttct > 2:452631/1‑100 (MQ=255) gggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttct < 1:233076/100‑1 (MQ=255) gggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttct < 2:1049697/100‑1 (MQ=255) ggACGACAACCTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTa < 2:463877/100‑1 (MQ=255) ggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTa < 2:1146470/100‑1 (MQ=255) ggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTa > 2:952843/1‑100 (MQ=255) caTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTc > 2:463923/1‑100 (MQ=255) caTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTc > 2:461928/1‑100 (MQ=255) caTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTc > 1:630543/1‑100 (MQ=255) tCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc > 2:577273/1‑100 (MQ=255) tCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc < 2:792850/100‑1 (MQ=255) tGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAg < 1:1241215/100‑1 (MQ=255) gCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGc > 1:1233789/1‑100 (MQ=255) cAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCa < 1:461931/100‑1 (MQ=255) aaaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTa < 1:1262714/100‑1 (MQ=255) gCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCTCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTg > 2:378750/1‑100 (MQ=255) cATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTgg > 1:135173/1‑100 (MQ=255) tatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCa < 1:728800/100‑1 (MQ=255) tGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGAt > 1:1120298/1‑100 (MQ=255) gTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATc < 2:851658/100‑1 (MQ=255) ttttAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCt > 1:593738/1‑100 (MQ=255) tAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTCTTTTCCCCGAGCAATTACGTGGCAGATCtttt > 1:1225167/1‑100 (MQ=255) tAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCtttt < 1:707492/100‑1 (MQ=255) agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc < 1:334355/100‑1 (MQ=255) agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc < 1:452633/100‑1 (MQ=255) agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc < 1:1004590/100‑1 (MQ=255) gagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCt > 2:1357247/1‑100 (MQ=255) | GGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT > NC_000913/36139‑36327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |