| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 428,188 | G→T | R181L (CGC→CTC) | secD → | SecYEG protein translocase auxillary subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 428,188 | 0 | G | T | 95.2% | 206.8 / 2.7 | 62 | R181L (CGC→CTC) | secD | SecYEG protein translocase auxillary subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (33/26); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG > NC_000913/428091‑428283 | cAAAATATCGATAGCCTACGCAGTCACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCctcg < 2:488502/100‑1 (MQ=255) aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg > 2:1200711/1‑100 (MQ=255) aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg < 1:290995/100‑1 (MQ=255) atatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc > 1:981539/1‑100 (MQ=255) agatCGATAGCCTACGGAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg < 2:427265/97‑1 (MQ=255) tatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCt < 2:994292/100‑1 (MQ=255) atCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTa > 2:717184/1‑100 (MQ=255) gATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACATTCCTCGATGCTAAAg > 2:920571/1‑100 (MQ=255) gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg > 1:376358/1‑100 (MQ=255) cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg < 1:1040479/100‑1 (MQ=255) cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg < 2:703668/100‑1 (MQ=255) tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga < 1:570269/100‑1 (MQ=255) cGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatg > 1:92956/1‑100 (MQ=255) cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGaa > 1:1171254/1‑100 (MQ=255) cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGaa > 2:391244/1‑100 (MQ=255) gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc > 1:1262553/1‑100 (MQ=255) ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt > 1:209390/1‑100 (MQ=255) ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt < 1:904548/100‑1 (MQ=255) tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc > 2:848386/1‑100 (MQ=255) tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc < 2:509339/100‑1 (MQ=255) gAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc < 2:1262544/100‑1 (MQ=255) aaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg < 1:538462/100‑1 (MQ=255) gggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGc > 2:849614/1‑100 (MQ=255) cATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa > 1:1233409/1‑98 (MQ=255) aTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgc < 1:355103/100‑1 (MQ=255) cccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAg < 1:1407056/97‑1 (MQ=255) tataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATc > 2:577204/1‑100 (MQ=255) ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa > 2:1143018/1‑100 (MQ=255) ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGAGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa > 2:854898/1‑100 (MQ=255) cACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAc < 1:502762/100‑1 (MQ=255) aCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc < 2:1174130/100‑1 (MQ=255) gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg > 2:632463/1‑100 (MQ=255) gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTg < 1:522588/99‑1 (MQ=255) ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGACATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt > 1:1102867/1‑100 (MQ=255) tCGTAAAGAATCCAACTACGGCCTGAGGATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTg < 2:937316/100‑1 (MQ=255) tCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTg < 2:962732/100‑1 (MQ=255) cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa > 1:519570/1‑100 (MQ=255) tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt > 2:584386/1‑100 (MQ=255) aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa > 2:1356516/1‑100 (MQ=255) aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag > 1:505202/1‑100 (MQ=255) gAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagca > 2:768487/1‑100 (MQ=255) gAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagca > 1:795265/1‑100 (MQ=255) aaaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag > 2:1011284/1‑100 (MQ=255) aaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcagc > 2:86904/1‑100 (MQ=255) aaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcagc < 1:768491/100‑1 (MQ=255) aacaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGcc > 1:1253436/1‑100 (MQ=255) aacaacTACGACCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGTGCGAGCGCCATCCGGACCTGGTGATTAGCAGcc < 2:1034051/100‑1 (MQ=255) acaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGCCCTGGTGATTcgcagcaa > 1:289477/1‑98 (MQ=255) cTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgc < 1:391245/100‑1 (MQ=255) aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag < 2:1396583/100‑1 (MQ=255) cGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagc < 2:1330857/100‑1 (MQ=255) ggCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca > 1:144213/1‑100 (MQ=255) ggCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca > 1:144238/1‑100 (MQ=255) gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCaa < 2:609086/100‑1 (MQ=255) ccTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAc < 2:727641/100‑1 (MQ=255) cTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc < 2:1105508/100‑1 (MQ=255) aTCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgc < 1:920579/100‑1 (MQ=255) tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg > 1:1292530/1‑100 (MQ=255) tCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg > 1:741451/1‑100 (MQ=255) aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg > 1:706199/1‑100 (MQ=255) cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc < 2:748409/100‑1 (MQ=255) tttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgcg < 1:118618/100‑1 (MQ=255) ttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCgg > 1:1429617/1‑100 (MQ=255) | CAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG > NC_000913/428091‑428283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |