Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	520,993	T→G	V193G (GTC→GGC)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	520,993	0	T	G	100.0%	184.4 / NA	52	V193G (GTC→GGC)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/24);  total (28/24)

GGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGG  >  NC_000913/520898‑521089                                                                                                |                                                                                                 ggATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGGATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCAcc                                                                                              >  1:735297/1‑100 (MQ=255) ggATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCAcc                                                                                              <  2:751693/100‑1 (MQ=255)     tCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGc                                                                                          >  1:648661/1‑100 (MQ=255)       cGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGt                                                                                        <  2:34842/100‑1 (MQ=255)       cGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGt                                                                                        <  2:446558/100‑1 (MQ=255)        ggTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAAGCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGtt                                                                                       >  1:1097807/1‑100 (MQ=255)        ggTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGtt                                                                                       <  2:1175437/100‑1 (MQ=255)         gTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTta                                                                                      <  1:1155083/100‑1 (MQ=255)             tAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATaaa                                                                                  <  1:1325352/100‑1 (MQ=255)              aaCCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAAt                                                                                 >  2:259243/1‑100 (MQ=255)                 cccTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcg                                                                              >  2:1322882/1‑100 (MQ=255)                  ccTGCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcgg                                                                             <  2:1063801/100‑1 (MQ=255)                    ttCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTcggcg                                                                           <  1:423084/100‑1 (MQ=255)                        aGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCaa                                                                       >  1:309698/1‑100 (MQ=255)                           tGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACga                                                                    <  2:731475/100‑1 (MQ=255)                           tGGCTCCGCGTCTGATGATGAATCTGGCGGATGGCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACga                                                                    >  2:1034436/1‑100 (MQ=255)                             gCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACgaga                                                                  <  2:1132420/100‑1 (MQ=255)                               tCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAAc                                                                >  1:218442/1‑100 (MQ=255)                                ccGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAAAGAGaa                                                                 <  2:1266605/98‑1 (MQ=255)                                    gTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCt                                                           <  1:361666/100‑1 (MQ=255)                                        gatgatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGAc                                                       >  1:799165/1‑100 (MQ=255)                                          tgatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACg                                                      >  2:1365728/1‑99 (MQ=255)                                           gatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGc                                                    >  1:189347/1‑100 (MQ=255)                                           gatgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGc                                                    >  1:1392191/1‑100 (MQ=255)                                             tgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTCTAAATGCGGCGGCAACGAGAACCAGCTCg                                                         >  2:1261919/1‑93 (MQ=255)                                             tgaATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTa                                                  <  1:1322891/100‑1 (MQ=255)                                                  cTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATgaga                                             >  1:184882/1‑100 (MQ=255)                                                       ggATGTCGATAAAGCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATgg                                        <  2:313446/100‑1 (MQ=255)                                                          tGTCGATAAAACCGGAGCCGTGCAGCCGGGGATTCGGGGGACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgttg                                     <  2:1103236/100‑1 (MQ=255)                                                           gTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGgttgt                                    >  2:980988/1‑100 (MQ=255)                                                             cGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTa                                  >  1:1493786/1‑100 (MQ=255)                                                             cGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGAGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTAGACGGCTATGAGaaa                                           >  2:17986/1‑91 (MQ=255)                                                              gATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGTCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTAc                                 >  2:856004/1‑100 (MQ=255)                                                                  aaaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCa                             <  1:447421/100‑1 (MQ=255)                                                                  aaaCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCa                             >  2:1322943/1‑100 (MQ=255)                                                                    aCCGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGc                           <  1:1473944/100‑1 (MQ=255)                                                                     ccGGAGCCGTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCt                          <  1:740430/100‑1 (MQ=255)                                                                             gTGCAGCCGGGGAGTCGGGGCACCTGGCGTTATGAATTCGGCGGCAACGAGGACCAGCTGGGAGGCTATGGGAAATGGTGGTTAGGTCAGCTTAAAc                     >  2:1150319/1‑97 (MQ=255)                                                                               gCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaa                >  1:1251115/1‑100 (MQ=255)                                                                               gCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaa                >  1:450894/1‑100 (MQ=255)                                                                               gCAGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaa                >  1:961935/1‑100 (MQ=255)                                                                               gCAGCCGGGGAGTCGGGGCACCTGGCGTGATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGa                 <  2:198691/99‑1 (MQ=255)                                                                                 aGCCGGGGAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaaca              <  2:218441/100‑1 (MQ=255)                                                                                  gCCGGGGAGTCGGGGGACGTGGCGGTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGaac               <  2:736442/98‑1 (MQ=255)                                                                                     ggggAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGc          >  2:1187536/1‑100 (MQ=255)                                                                                      gggAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCt         <  2:1086433/100‑1 (MQ=255)                                                                                      gggAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAAGGGTTGTTACCGCAGCTTAAACCCGAACAACGCt         >  2:1312241/1‑100 (MQ=255)                                                                                      gggAGTCGGGGCACCTGGCGCTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCt         <  2:804888/100‑1 (MQ=255)                                                                                       ggAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTg        >  1:1321279/1‑100 (MQ=255)                                                                                        gAGTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTgg       <  2:21836/100‑1 (MQ=255)                                                                                          gTCGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGt      >  2:1446149/1‑99 (MQ=255)                                                                                            cGGGGCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGATTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACg   >  2:935757/1‑100 (MQ=255)                                                                                             ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACgg  >  1:68442/1‑100 (MQ=255)                                                                                             ggggCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACgg  >  1:1093319/1‑100 (MQ=255)                                                                                                |                                                                                                 GGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGG  >  NC_000913/520898‑521089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑