Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,180	G→A	L202L (CTG→TTG)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,180	0	G	A	98.2%	197.0 / ‑5.2	57	L202L (CTG→TTG)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/24);  minor base C (0/1);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTT  >  NC_000913/674085‑674275                                                                                                |                                                                                                ggTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCg                                                                                             >  1:1464308/1‑100 (MQ=255)      tctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGc                                                                                        >  2:631172/1‑100 (MQ=255)         ccACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTa                                                                                     >  2:942768/1‑100 (MQ=255)           aCGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAg                                                                                   >  2:895848/1‑100 (MQ=255)             gCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCa                                                                                 <  2:728026/100‑1 (MQ=255)                  cGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAt                                                                            >  2:18785/1‑100 (MQ=255)                     aaCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGAtttt                                                                         <  2:687411/100‑1 (MQ=255)                        gACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGAt                                                                      >  1:168115/1‑100 (MQ=255)                         aCCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATa                                                                     >  2:1318948/1‑100 (MQ=255)                          ccGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATaa                                                                    <  1:1569497/100‑1 (MQ=255)                          ccGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATa                                                                     <  1:1168390/99‑1 (MQ=255)                           cGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATaaa                                                                   <  2:1287571/100‑1 (MQ=255)                            gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGATGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAAc                                                                  >  2:855003/1‑100 (MQ=255)                                     aCGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCggg                                                         >  1:1001651/1‑100 (MQ=255)                                     aCGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCggg                                                         <  2:393822/100‑1 (MQ=255)                                        cTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGAtc                                                      >  1:890107/1‑100 (MQ=255)                                          gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGAtctc                                                    >  2:1563168/1‑100 (MQ=255)                                          gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGAtctc                                                    >  1:1218990/1‑100 (MQ=255)                                           cATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATctct                                                   <  1:88037/100‑1 (MQ=255)                                             tGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCttt                                                 <  2:956512/100‑1 (MQ=255)                                             tGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCttt                                                 >  2:910336/1‑100 (MQ=255)                                              ggTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTa                                                >  1:375911/1‑100 (MQ=255)                                                ttttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACg                                              <  1:735439/100‑1 (MQ=255)                                                 tttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGt                                             <  2:541482/100‑1 (MQ=255)                                                 tttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGt                                             >  1:1237462/1‑100 (MQ=255)                                                 tttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGt                                             <  2:935892/100‑1 (MQ=255)                                                  ttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGtt                                            >  2:1504036/1‑100 (MQ=255)                                                  ttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGtt                                            >  1:1122641/1‑100 (MQ=255)                                                   tAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTc                                           >  2:883206/1‑100 (MQ=255)                                                   tAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTc                                           >  2:12999/1‑100 (MQ=255)                                                   tAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTc                                           >  1:1455816/1‑100 (MQ=255)                                                           tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                   <  1:1096988/100‑1 (MQ=255)                                                           tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                   <  1:937018/100‑1 (MQ=255)                                                            cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGt                                  <  1:31982/100‑1 (MQ=255)                                                            cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGt                                  <  2:1001463/100‑1 (MQ=255)                                                            cTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGt                                  >  2:460536/1‑100 (MQ=255)                                                             tGGCCAGTGATCCAGTCTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTa                                 <  1:311475/100‑1 (MQ=255)                                                              ggCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTAt                                >  1:1172408/1‑100 (MQ=255)                                                               gCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTAt                                >  1:1264911/1‑99 (MQ=255)                                                               gCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATc                               >  2:534214/1‑100 (MQ=255)                                                                 cAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGc                             >  2:1023867/1‑100 (MQ=255)                                                                   gTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAg                           <  1:895856/100‑1 (MQ=255)                                                                     gATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAgcg                         <  2:307965/100‑1 (MQ=255)                                                                     gATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAgcg                         <  1:944008/100‑1 (MQ=255)                                                                       tCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGcc                       >  1:247672/1‑100 (MQ=255)                                                                             ttATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagc                 >  1:43602/1‑100 (MQ=255)                                                                              tATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagca                >  1:502011/1‑100 (MQ=255)                                                                                tCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCagcagcagc              <  2:432263/100‑1 (MQ=255)                                                                                  cAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCg            <  2:1455806/100‑1 (MQ=255)                                                                                  cAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCg            >  1:766678/1‑100 (MQ=255)                                                                                   aGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGt           >  1:869956/1‑100 (MQ=255)                                                                                    gATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc          <  1:910344/100‑1 (MQ=255)                                                                                    gATCGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc          <  2:1237455/100‑1 (MQ=255)                                                                                      tCGTTGAGCACCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCACCTCTGGTATCGCAGCGCCAGCAGCAGCCGTCGa        <  2:1033174/100‑1 (MQ=255)                                                                                       cGTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGAt       <  2:1348298/100‑1 (MQ=255)                                                                                        gTTGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATa      >  1:852839/1‑100 (MQ=255)                                                                                         ttGAGCAACTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATaa     >  1:1394246/1‑100 (MQ=255)                                                                                            agcaacTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACtt  <  1:328843/100‑1 (MQ=255)                                                                                                |                                                                                                GGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTT  >  NC_000913/674085‑674275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑