Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,805	A→T	C264S (TGC→AGC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,805	0	A	T	100.0%	174.4 / NA	51	C264S (TGC→AGC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/31);  total (20/31)

GCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC  >  NC_000913/700710‑700900                                                                                                |                                                                                                gCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCa                                                                                             <  1:1451691/100‑1 (MQ=255)      cgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGtt                                                                                        >  2:92776/1‑100 (MQ=255)       gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                       >  2:317774/1‑100 (MQ=255)          cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                    <  1:873962/100‑1 (MQ=255)                 aCGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGc                                                                             <  2:89797/100‑1 (MQ=255)                 aCGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGc                                                                             <  2:1491965/100‑1 (MQ=255)                  cgcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCa                                                                            >  1:719921/1‑100 (MQ=255)                           ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc                                                                   <  1:1552351/100‑1 (MQ=255)                               gCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGc                                                               <  2:1144939/100‑1 (MQ=255)                               gCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGc                                                               <  2:523301/100‑1 (MQ=255)                                cccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCg                                                              >  1:368175/1‑100 (MQ=255)                                     cTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTTGCAGCGTTCACCCAGCGGTTCg                                                         >  2:369277/1‑100 (MQ=255)                                      tctgtcAGATTCAACACCCTTTGTTTTATGGCAGCGTTTGCAGCGTGAGTTCCCAGGCTGCCAAATTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGa                                                        <  2:303359/95‑1 (MQ=255)                                       ttAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAc                                                       >  2:149288/1‑100 (MQ=255)                                       ttAACAGAATCCACCCCCGTTGTTCAATGGCAGCTTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCCCAGTGGCAGCGTTCACCCAGCCGTTCGAc                                                       <  1:165889/100‑1 (MQ=255)                                        taccAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                      <  2:183419/97‑1 (MQ=255)                                          aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                                    <  1:442680/100‑1 (MQ=255)                                           cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGTCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                   >  1:1438091/1‑100 (MQ=255)                                           cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                   >  1:536761/1‑100 (MQ=255)                                           cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                   <  1:820413/100‑1 (MQ=255)                                            aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                  <  2:1376819/100‑1 (MQ=255)                                             gATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                                 <  2:425133/100‑1 (MQ=255)                                              aTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata                                                <  1:1139880/100‑1 (MQ=255)                                                 cAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATgg                                             >  1:346105/1‑100 (MQ=255)                                                      cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                        <  1:810955/100‑1 (MQ=255)                                                      cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                        <  2:200004/100‑1 (MQ=255)                                                       ccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAtt                                       <  2:979424/100‑1 (MQ=255)                                                       ccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAtt                                       >  2:1020185/1‑100 (MQ=255)                                                                  tGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgtt                            >  2:1224131/1‑100 (MQ=255)                                                                  tGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgtt                            >  1:90030/1‑100 (MQ=255)                                                                  gggCAGCGTTGGCAGCGATAGTTTCCAGGGTGCCAAATTTGCCGCAGTGGCAGCGTTCACGCAGCGGTTCGACCTTATTATGGCCAATTTCACCGa                                >  2:1334161/2‑96 (MQ=255)                                                                   ggCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttg                           >  1:282602/1‑100 (MQ=255)                                                                    gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc                          >  1:1216167/1‑100 (MQ=255)                                                                     cAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgcc                         <  1:1482836/100‑1 (MQ=255)                                                                       gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgt                       >  1:1195464/1‑100 (MQ=255)                                                                         gTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCCCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgttg                     <  1:1241902/100‑1 (MQ=255)                                                                           tGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCg                   <  2:1116794/100‑1 (MQ=255)                                                                              cAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGcc                <  1:1527136/100‑1 (MQ=255)                                                                              cAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGc                 <  2:1566822/99‑1 (MQ=255)                                                                               aGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCa               <  2:506621/100‑1 (MQ=255)                                                                                gCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCaa              >  1:488569/1‑100 (MQ=255)                                                                                 cGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGCTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAAt             <  1:399046/100‑1 (MQ=255)                                                                                       tttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAAt       <  1:149288/100‑1 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg      <  1:604676/100‑1 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg      <  2:1147758/100‑1 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg      >  1:1145879/1‑100 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg      >  1:10069/1‑100 (MQ=255)                                                                                          ccAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcg    <  2:536758/100‑1 (MQ=255)                                                                                          ccAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcg    <  2:90030/100‑1 (MQ=255)                                                                                            aGGCTGCCAAATTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc  <  2:511000/100‑1 (MQ=255)                                                                                            aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc  >  2:1166002/1‑100 (MQ=255)                                                                                                |                                                                                                GCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC  >  NC_000913/700710‑700900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑