| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 772,209 | C→A | T251N (ACC→AAC) | cydA → | cytochrome d terminal oxidase, subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 772,209 | 0 | C | A | 98.4% | 222.5 / ‑3.4 | 64 | T251N (ACC→AAC) | cydA | cytochrome d terminal oxidase, subunit I |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (33/30); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACAA > NC_000913/772114‑772305 | cTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACaaa > 1:467867/1‑100 (MQ=255) tGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTg > 2:1180776/1‑100 (MQ=255) gCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTgg > 2:1040466/1‑100 (MQ=255) tATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgct > 1:816344/1‑100 (MQ=255) tCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgctgc > 1:477312/1‑100 (MQ=255) tGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAtt < 1:543184/100‑1 (MQ=255) gCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTg > 2:243802/1‑100 (MQ=255) gCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTg < 2:561502/100‑1 (MQ=255) ccAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGa > 1:222067/1‑100 (MQ=255) cAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGaa < 2:467864/100‑1 (MQ=255) gCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGc < 1:529354/100‑1 (MQ=255) cTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGcc > 1:56245/1‑100 (MQ=255) ttCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCg > 1:232355/1‑100 (MQ=255) ggTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGt > 1:795376/1‑100 (MQ=255) ggCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGaa > 1:432706/1‑100 (MQ=255) ggCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGaa > 2:338520/1‑100 (MQ=255) ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc < 2:1120203/100‑1 (MQ=255) ctgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAAc < 1:1400515/100‑1 (MQ=255) gctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGc < 2:345977/100‑1 (MQ=255) gctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGc < 1:1180783/100‑1 (MQ=255) ttCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAcc < 1:858720/100‑1 (MQ=255) tctgtctgTTATTGTTATGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGTCTGCTATTGAAGCCGAGTGGGAAACGCAAcc < 1:624262/99‑1 (MQ=255) gtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTgcg < 2:1262831/100‑1 (MQ=255) ctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGcc > 2:164066/1‑100 (MQ=255) ctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGcc > 2:1164982/1‑100 (MQ=255) ctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGcc > 2:1164975/1‑100 (MQ=255) tgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct > 1:842175/1‑100 (MQ=255) gtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctg > 2:784656/1‑100 (MQ=255) tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgct > 1:559822/1‑100 (MQ=255) tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgct > 1:1295929/1‑100 (MQ=255) aTTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgctg < 1:1215885/100‑1 (MQ=255) ttGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCtgctgc > 2:446679/1‑100 (MQ=255) ttGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCtgctgc > 1:199863/1‑100 (MQ=255) tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc < 2:433736/100‑1 (MQ=255) tCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCttt < 1:695271/100‑1 (MQ=255) cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa < 1:1040473/100‑1 (MQ=255) cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa > 1:1216226/1‑100 (MQ=255) cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa > 1:417579/1‑100 (MQ=255) cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa > 1:346481/1‑100 (MQ=255) tGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc > 1:1336868/1‑100 (MQ=255) tgatgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTg < 2:164482/100‑1 (MQ=255) tgatgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTg < 2:816339/100‑1 (MQ=255) tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc < 2:1162936/100‑1 (MQ=255) tgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTc > 2:316905/1‑100 (MQ=255) gaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCg > 2:1345856/1‑100 (MQ=255) gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc > 2:759441/1‑100 (MQ=255) gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCAAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc < 1:364164/100‑1 (MQ=255) cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt < 2:402171/100‑1 (MQ=255) tACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTg > 1:965079/1‑100 (MQ=255) tACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTg < 1:1393764/100‑1 (MQ=255) cGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGAt < 1:1102355/100‑1 (MQ=255) aaaTGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCa < 2:669672/100‑1 (MQ=255) aaaTGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCa > 1:704609/1‑100 (MQ=255) aTGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAg < 2:250977/99‑1 (MQ=255) gggCGACGTGCAGAAAAACAAACTGGCTGCTATGGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGACTTTACTCTGTTCGGCATTCCTGATCAGTaa < 2:232354/100‑1 (MQ=255) cGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAagag < 2:417578/100‑1 (MQ=255) gACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgagg < 1:522903/100‑1 (MQ=255) tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc > 1:908643/1‑100 (MQ=255) tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc < 2:1156689/100‑1 (MQ=255) cAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGa > 1:678482/1‑100 (MQ=255) aGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAgaagaa > 2:774431/1‑100 (MQ=255) aGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGaa < 2:1214714/100‑1 (MQ=255) aGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGaa < 2:963775/100‑1 (MQ=255) gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc > 2:59472/1‑100 (MQ=255) aaaaCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACaa > 1:1476868/1‑100 (MQ=255) aaaaCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACaa > 1:1322121/1‑100 (MQ=255) aaaaCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATGCCTGAACGGGAAGAGGAGCCGAACa > 2:446814/1‑99 (MQ=255) | CTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACAA > NC_000913/772114‑772305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |