Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,100,233 | G→A | intergenic (+39/‑63) | ycdY → / → ycdZ | redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,100,233 | 0 | G | A | 93.1% | 179.8 / 3.5 | 58 | intergenic (+39/‑63) | ycdY/ycdZ | redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein |
Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (30/24); total (33/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
GCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA > NC_000913/1100142‑1100328 | gcgATGCCATTAGTGCAATGTGGGACGAGCTGAAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGc < 1:864047/99‑1 (MQ=255) tGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaa > 2:660565/1‑100 (MQ=255) ccATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAt < 1:307948/100‑1 (MQ=255) cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg > 2:381872/1‑100 (MQ=255) cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg > 1:645135/1‑100 (MQ=255) ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg < 2:1451484/100‑1 (MQ=255) ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg < 2:1449468/100‑1 (MQ=255) ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg < 1:758120/100‑1 (MQ=255) tcaGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg < 1:812240/98‑1 (MQ=255) tAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgt < 1:1342862/100‑1 (MQ=255) gggaaTGTGGGAGGGGCTGGAAGAAGATTTTGAAGAATAAGTGTGGTATACGTCACTCATAGCTGCGACTTATGATTTGTTGGGGCATAAAAtgtg < 2:732948/93‑1 (MQ=255) gCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc > 2:913480/1‑100 (MQ=255) gCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc > 1:1083867/1‑100 (MQ=255) aTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAt > 1:1358369/1‑100 (MQ=255) tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc < 2:148973/100‑1 (MQ=255) tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc < 1:620146/100‑1 (MQ=255) gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct < 1:1273634/100‑1 (MQ=255) gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct > 2:1063459/1‑100 (MQ=255) ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat > 2:1207167/1‑100 (MQ=255) ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat < 1:1159320/100‑1 (MQ=255) ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat < 2:191474/100‑1 (MQ=255) gCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATgg < 2:504414/100‑1 (MQ=255) agaagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGt > 1:787880/1‑100 (MQ=255) agaagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGt > 2:1462198/1‑100 (MQ=255) gaagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtt > 2:440171/1‑100 (MQ=255) agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt > 2:564031/1‑100 (MQ=255) gaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTc < 1:564034/100‑1 (MQ=255) tCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGc < 1:1093235/100‑1 (MQ=255) cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt > 2:1246762/1‑100 (MQ=255) cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt > 2:154698/1‑100 (MQ=255) cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt > 1:412976/1‑100 (MQ=255) gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt < 1:904381/100‑1 (MQ=255) gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt > 2:177900/1‑100 (MQ=255) gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt > 2:104840/1‑100 (MQ=255) gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt > 2:1070967/1‑100 (MQ=255) gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt > 2:855417/1‑100 (MQ=255) gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt > 1:760874/1‑100 (MQ=255) gtgtGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg < 1:1545119/100‑1 (MQ=255) gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg > 2:1411266/1‑100 (MQ=255) aTCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGa < 1:1078138/100‑1 (MQ=255) tCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa > 1:9227/1‑100 (MQ=255) cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc < 1:148947/100‑1 (MQ=255) tACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACa > 1:438570/1‑100 (MQ=255) tCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt > 2:617939/1‑100 (MQ=255) tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt > 1:587301/1‑100 (MQ=255) cTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct > 2:782093/1‑100 (MQ=255) cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctct > 1:310050/1‑100 (MQ=255) cTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt > 1:383917/1‑100 (MQ=255) tGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg < 1:1403914/100‑1 (MQ=255) tGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg < 1:1127353/100‑1 (MQ=255) gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc < 1:514460/100‑1 (MQ=255) cAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCa > 1:1194659/1‑100 (MQ=255) cAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCa > 1:1048094/1‑100 (MQ=255) ggATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATca > 1:18763/1‑100 (MQ=255) aTAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcaca < 1:1357104/100‑1 (MQ=255) aaTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaac > 1:40762/1‑100 (MQ=255) aaTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaac < 2:79701/100‑1 (MQ=255) aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaaca > 2:793930/1‑100 (MQ=255) | GCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA > NC_000913/1100142‑1100328 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |