Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,125	C→T	D415N (GAC→AAC)	rne ←	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,125	0	C	T	100.0%	159.6 / NA	45	D415N (GAC→AAC)	rne	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/26);  total (19/26)

ATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCC  >  NC_000913/1143028‑1143221                                                                                                  |                                                                                                 aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtac                                                                                                <  2:1235868/100‑1 (MQ=255) aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtac                                                                                                <  2:443284/100‑1 (MQ=255)     gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcac                                                                                            <  1:929332/100‑1 (MQ=255)     gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcac                                                                                            <  2:207406/100‑1 (MQ=255)      cacaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcacg                                                                                           >  1:1038493/1‑100 (MQ=255)       acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGCATCGAGAGCGACAGCGATTCGTTGTTACGCACgg                                                                                          >  1:1283310/1‑100 (MQ=255)        caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCAAGGt                                                                                         >  1:129180/1‑100 (MQ=255)           gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc                                                                                      <  1:915364/100‑1 (MQ=255)                 tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAcc                                                                                <  1:1226812/100‑1 (MQ=255)                 tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAcc                                                                                <  1:1491404/100‑1 (MQ=255)                 tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAcc                                                                                <  2:1114077/100‑1 (MQ=255)                   gCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAg                                                                              <  1:488221/100‑1 (MQ=255)                    cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGa                                                                             <  2:1160307/100‑1 (MQ=255)                          cTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcg                                                                       <  1:1030393/100‑1 (MQ=255)                            tCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcgc                                                                      >  2:725352/1‑99 (MQ=255)                               tGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGAc                                                                  >  1:387546/1‑100 (MQ=255)                                      tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg                                                           >  1:1195036/1‑100 (MQ=255)                                       ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          >  2:811186/1‑100 (MQ=255)                                        tctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgat                                                         <  1:1097984/100‑1 (MQ=255)                                        tctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg                                                           >  2:622782/1‑98 (MQ=255)                                         ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg                                                        >  1:701847/1‑100 (MQ=255)                                         ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg                                                        <  1:889220/100‑1 (MQ=255)                                           ttCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGAc                                                      <  2:387545/100‑1 (MQ=255)                                             cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg                                                    <  2:1444205/100‑1 (MQ=255)                                              aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg                                                   >  1:1197858/1‑100 (MQ=255)                                                cgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                 >  1:1126784/1‑100 (MQ=255)                                                cgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                 <  2:625975/100‑1 (MQ=255)                                                 gcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAtt                                                >  1:331757/1‑100 (MQ=255)                                                    tcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAc                                             >  2:299293/1‑100 (MQ=255)                                                     cttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                            >  2:216285/1‑100 (MQ=255)                                                         ttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                        <  1:1541468/100‑1 (MQ=255)                                                         ttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                        <  1:1533398/100‑1 (MQ=255)                                                          tcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTg                                       <  1:570404/100‑1 (MQ=255)                                                            gATCAGACGCAGAATAGAGAGCGACAGCGATTTGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGAt                                     >  2:1364086/1‑100 (MQ=255)                                                             aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                    <  2:519518/100‑1 (MQ=255)                                                                     cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                            >  2:1129015/1‑100 (MQ=255)                                                                     cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                            <  1:55962/100‑1 (MQ=255)                                                                     cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                            >  1:1199600/1‑100 (MQ=255)                                                                      aGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgc                           >  2:1235169/1‑100 (MQ=255)                                                                      aGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgc                           <  1:396019/100‑1 (MQ=255)                                                                              gagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACgg                   <  1:1554546/100‑1 (MQ=255)                                                                               agCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                  <  2:1192266/100‑1 (MQ=255)                                                                                gCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACgggn                 <  1:1311228/100‑2 (MQ=255)                                                                                gCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGa                 <  1:24526/100‑1 (MQ=255)                                                                                 cGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc                <  2:539129/100‑1 (MQ=255)                                                                                   aCAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAt              >  1:875334/1‑100 (MQ=255)                                                                                       cGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc          <  1:216286/100‑1 (MQ=255)                                                                                        gATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCca         <  1:761700/100‑1 (MQ=255)                                                                                        gATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCca         <  1:20738/100‑1 (MQ=255)                                                                                             gttgttACGCACGGTGCCAGTACCAGAACAACGCGGACGAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAgg    >  2:968026/1‑100 (MQ=255)                                                                                             gttgttACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAgg    >  1:1379243/1‑100 (MQ=255)                                                                                               tGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGcc  >  1:1113204/1‑100 (MQ=255)                                                                                                  |                                                                                                 ATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCC  >  NC_000913/1143028‑1143221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑