| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,325,850 | C→T | R369C (CGC→TGC) | yciQ → | enhancer of membrane protein expression; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,325,850 | 0 | C | T | 96.9% | 213.3 / ‑4.1 | 64 | R369C (CGC→TGC) | yciQ | enhancer of membrane protein expression; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (29/33); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGC > NC_000913/1325754‑1325943 | ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCa < 1:471395/100‑1 (MQ=255)ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCa > 1:377333/1‑100 (MQ=255) gcgcGACCAGAAATAATGCGGTATTACTGCCCGCAGCGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGTGGAGATGCaa > 2:681712/1‑100 (MQ=255) gcgACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACa > 2:850828/1‑100 (MQ=255) gcgACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCCTACGGGGAGATGCAACa > 1:283230/1‑100 (MQ=255) cgACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACag < 2:1239044/100‑1 (MQ=255) atcAGAAATAATGCGGTATTACTGCTCGGAGAGCAGATATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACagag < 2:135267/98‑1 (MQ=255) cAGAAATAATGCGGTATTACTGCGCGCAGAGCAGTGATTACTGGAAGCGTTGTTTCGTAAAGGCGATAGGGTCGTTCTTACGTGGAGATGCAACAGAATc > 1:340997/1‑100 (MQ=255) aGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCt > 2:1109473/1‑100 (MQ=255) gAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGATATGCAACAGAGTCtt > 1:1546132/1‑100 (MQ=255) gAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCtt < 2:611305/100‑1 (MQ=255) gAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCtt > 1:1046159/1‑100 (MQ=255) aaaTAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTa < 2:516217/100‑1 (MQ=255) ataatGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACg > 1:31828/1‑100 (MQ=255) tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAgg > 2:656246/1‑100 (MQ=255) gCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAggg > 2:584260/1‑100 (MQ=255) gCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAggg < 2:1190364/100‑1 (MQ=255) cGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGc < 2:1121268/100‑1 (MQ=255) tATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCAtt > 2:1520269/1‑100 (MQ=255) tACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCAttttt < 2:81710/100‑1 (MQ=255) tACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCAttttt < 2:805514/100‑1 (MQ=255) gCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGTAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATg < 2:669656/99‑1 (MQ=255) cAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCa < 1:823119/100‑1 (MQ=255) agagCAGTTATTACTGGAAGGGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAg < 2:1408898/100‑1 (MQ=255) gCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaaa > 1:259255/1‑100 (MQ=255) aGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtt > 1:507626/1‑100 (MQ=255) tattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa > 1:1155262/1‑100 (MQ=255) ttaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATc < 1:1458211/100‑1 (MQ=255) tGGTAGCGTTATTCCGTAAAGGCGATAAGGTCGTTCTTTTGGTGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGcc < 2:1281730/100‑1 (MQ=255) tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGcc < 2:1282041/100‑1 (MQ=255) ggAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg < 2:239666/100‑1 (MQ=255) gcaaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg > 1:1064137/3‑100 (MQ=255) gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc < 1:1569334/100‑1 (MQ=255) cGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGCGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTa < 1:1098468/99‑1 (MQ=255) gTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaa > 1:983678/1‑100 (MQ=255) gTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaa > 1:1081035/1‑100 (MQ=255) aTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAg < 1:494455/100‑1 (MQ=255) ttCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTc < 1:850834/100‑1 (MQ=255) ttAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTc < 1:1207771/99‑1 (MQ=255) tAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTca > 2:1207764/1‑99 (MQ=255) tAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCg < 2:507623/100‑1 (MQ=255) tAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCg < 1:210598/100‑1 (MQ=255) aaaGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGt < 1:537426/100‑1 (MQ=255) aaGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCTGCGTAAAAAGTCTTCGtt < 2:470272/100‑1 (MQ=255) aaGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGtt < 2:470393/100‑1 (MQ=255) ggCGCTAAGGTCGTTCTTCAGGGGCGACGAAATAGAGTATTACGCAGGGCATTTTTACGGATGCAGAAATTTGATCTGCCGCGGAAAAAGGCTTCTtttt > 2:1469707/1‑100 (MQ=255) gATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAcc < 1:1253101/100‑1 (MQ=255) aGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTc > 2:1554413/1‑100 (MQ=255) aGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTc < 2:1573875/100‑1 (MQ=255) ggTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt < 1:265868/100‑1 (MQ=255) ggTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt > 2:265867/1‑100 (MQ=255) ggTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt > 1:772389/1‑100 (MQ=255) gctCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt > 1:1067366/3‑100 (MQ=255) gTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTg > 1:572065/1‑100 (MQ=255) gTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTg > 2:82685/1‑100 (MQ=255) tCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGa < 1:238618/100‑1 (MQ=255) gTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATa > 1:488427/1‑100 (MQ=255) ttCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATAc < 1:656249/100‑1 (MQ=255) cTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACtt > 2:313669/1‑100 (MQ=255) cTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACtt > 2:232411/1‑100 (MQ=255) ttACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACttt > 1:335574/1‑100 (MQ=255) ggggAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTg < 1:1247737/100‑1 (MQ=255) gggAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGc > 1:937619/1‑100 (MQ=255) gggAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGc < 1:32147/100‑1 (MQ=255) | GGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGC > NC_000913/1325754‑1325943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |