Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	222,731	C→T	intergenic (‑86/‑102)	metN ← / → gmhB	DL‑methionine transporter subunit/D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	222,731	0	C	T	100.0%	167.9 / NA	49	intergenic (‑86/‑102)	metN/gmhB	DL‑methionine transporter subunit/D,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/28);  total (21/28)

AGTTTTATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGCATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCTTATTAAGGAGCTA  >  NC_000913/222635‑222826                                                                                                 |                                                                                                aGTTTTATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTAt                                                                                               <  2:239915/99‑1 (MQ=255)   ttttATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCa                                                                                            >  1:1235121/1‑100 (MQ=255)   ttttATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCa                                                                                            >  2:1194772/1‑100 (MQ=255)     ttATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGa                                                                                          <  1:1390356/100‑1 (MQ=255)      tATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGAc                                                                                         >  2:1490208/1‑100 (MQ=255)       aTCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACg                                                                                        >  2:614426/1‑100 (MQ=255)       aTCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACg                                                                                        <  2:1235114/100‑1 (MQ=255)         cATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAGTGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTc                                                                                      >  2:754671/1‑100 (MQ=255)         cATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTc                                                                                      <  2:1519087/100‑1 (MQ=255)              attattTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAaat                                                                                 <  1:1485502/100‑1 (MQ=255)               tgatttattATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAaatc                                                                                <  2:838175/98‑1 (MQ=255)                 atttattATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAaatcaa                                                                              >  1:320680/1‑100 (MQ=255)                    tattatCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAatcaatca                                                                           >  2:996512/1‑100 (MQ=255)                       tatCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAgg                                                                         >  1:126271/1‑99 (MQ=255)                       tatCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGt                                                                        >  2:570682/1‑100 (MQ=255)                        atCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGtt                                                                       <  2:740256/100‑1 (MQ=255)                        atCGGCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGtt                                                                       <  1:1386537/100‑1 (MQ=255)                          cGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTa                                                                     >  1:1456625/1‑100 (MQ=255)                              aTTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCg                                                                 >  1:1234921/1‑100 (MQ=255)                                tAAGTTAGTCGTGGCATGTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGaa                                                               <  1:925551/100‑1 (MQ=255)                                  aGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAaga                                                             >  1:1497647/1‑100 (MQ=255)                                   gTTAGTCGTGGAATGTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAagag                                                            <  2:158655/100‑1 (MQ=255)                                        tCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACtt                                                       <  2:1002416/100‑1 (MQ=255)                                               aTCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCa                                                <  2:453020/100‑1 (MQ=255)                                                        gCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAAc                                       <  1:1128866/100‑1 (MQ=255)                                                         ccTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACa                                      >  2:994205/1‑100 (MQ=255)                                                         ccTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACa                                      >  2:1377236/1‑100 (MQ=255)                                                         ccTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACa                                      >  2:3217/1‑100 (MQ=255)                                                          cTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACAt                                     >  1:744826/1‑100 (MQ=255)                                                          cTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACAt                                     >  2:182145/1‑100 (MQ=255)                                                           tGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATg                                    <  2:1109351/100‑1 (MQ=255)                                                            gAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGc                                   <  1:754675/100‑1 (MQ=255)                                                             aaaCGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCg                                  <  1:533363/100‑1 (MQ=255)                                                              aaCGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGa                                 <  1:1212690/100‑1 (MQ=255)                                                              aaCGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGa                                 >  2:973657/1‑100 (MQ=255)                                                               aCGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGAt                                <  2:832690/100‑1 (MQ=255)                                                                cGGGCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATa                               >  2:524276/1‑100 (MQ=255)                                                                   gCAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTa                            <  1:1344399/100‑1 (MQ=255)                                                                    cAACGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAg                           <  2:1497637/100‑1 (MQ=255)                                                                      aCGCCGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCg                         <  1:570685/100‑1 (MQ=255)                                                                          cGTCAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTca                      <  1:25647/99‑1 (MQ=255)                                                                             cAATGAAATGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATg                  <  2:126271/100‑1 (MQ=255)                                                                                  aaaTGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCtta             <  2:522835/100‑1 (MQ=255)                                                                                  aaaTGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCtta             <  2:1456615/100‑1 (MQ=255)                                                                                    aTGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCttatt           >  2:381011/1‑100 (MQ=255)                                                                                     tGGATGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGCAACATGCGATACTAGCGTCACATGCCttatta          <  1:1490218/100‑1 (MQ=255)                                                                                         tGTTAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCTTATTAAGGa      >  1:1055119/1‑100 (MQ=255)                                                                                           ttAAGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCTTATTAAGGAGc    <  1:1558128/100‑1 (MQ=255)                                                                                             aaGGTATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCTTATTAAGGAGCta  <  1:1196041/100‑1 (MQ=255)                                                                                                 |                                                                                                AGTTTTATCATTGATTATTTATTATCGTCATTAAGTTAGTCGTGGCATCTCGAATGCCTGAAACGGGCAACGCCGTCAATGAAATGGATGTTAAGGCATCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCACATGCCTTATTAAGGAGCTA  >  NC_000913/222635‑222826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑