Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,291,084 | A→C | K195N (AAA→AAC) | narP → | response regulator in two‑component regulatory system with NarQ |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,291,084 | 0 | A | C | 98.7% | 261.7 / ‑3.8 | 74 | K195N (AAA→AAC) | narP | response regulator in two‑component regulatory system with NarQ |
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (41/32); total (42/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 |
ACGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAG > NC_000913/2290987‑2291179 | aCGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCt < 1:543665/100‑1 (MQ=255) gAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCa < 2:226634/100‑1 (MQ=255) gCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAAt > 2:69694/1‑100 (MQ=255) cTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATg < 1:1405476/100‑1 (MQ=255) ggCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTc > 1:741251/1‑100 (MQ=255) ggCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTc > 2:993683/1‑100 (MQ=255) gCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTcc > 2:1002997/1‑100 (MQ=255) cacaGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCg > 2:812070/1‑100 (MQ=255) acaGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGc > 1:744627/1‑100 (MQ=255) acaGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGc > 1:742948/1‑100 (MQ=255) ggCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCAcg > 1:198896/1‑100 (MQ=255) gCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCAcgc < 2:1052195/100‑1 (MQ=255) gTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTg > 2:707492/1‑100 (MQ=255) tCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTgg > 2:737468/1‑100 (MQ=255) tCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTgg > 2:965246/1‑100 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTggc > 1:318453/1‑100 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTggc > 2:1066248/1‑100 (MQ=255) cAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTggc > 2:816653/1‑100 (MQ=255) aaaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGcca > 2:1472734/1‑100 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccac > 2:321531/1‑100 (MQ=255) aaCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccac < 2:351321/100‑1 (MQ=255) aCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccacc < 1:1560496/100‑1 (MQ=255) aCAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccacc > 2:799542/1‑100 (MQ=255) cAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGccacca > 1:291303/1‑100 (MQ=255) aGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCAt > 2:1473815/1‑100 (MQ=255) gATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCAtt < 2:854022/100‑1 (MQ=255) gATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCAtt > 1:1488437/1‑100 (MQ=255) aTTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTACCCTCAATGTCCGCTCACGCGTGGCGGCCACCATTc < 2:1146627/100‑1 (MQ=255) aTTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTc > 1:192372/1‑100 (MQ=255) tGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTg > 2:1240726/1‑100 (MQ=255) gCCTCGGTGTTGAATATGTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCt < 2:54533/98‑1 (MQ=255) ccgatctgtTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGtt < 2:1290867/94‑1 (MQ=255) tCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTcc < 1:581476/100‑1 (MQ=255) tgtTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGca < 1:816658/100‑1 (MQ=255) gtTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGttagatcgg > 1:1290876/1‑93 (MQ=255) tGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaaca < 1:799547/100‑1 (MQ=255) gAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACGTCAATGTCCGCTCACCCGTGGCGGACACCATTCTGTTCCTGcaacaa > 2:1061347/1‑100 (MQ=255) gAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaacaa > 2:735423/1‑100 (MQ=255) aaTATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGcaacaa > 2:1428421/1‑99 (MQ=255) atatTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAAcg > 2:58448/1‑100 (MQ=255) atTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAACGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAAcgcg < 2:553623/100‑1 (MQ=255) tttCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCGCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCgg > 1:601184/1‑100 (MQ=255) ccGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGc < 2:33697/100‑1 (MQ=255) cGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGca < 1:176876/100‑1 (MQ=255) cGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGc < 2:1198568/99‑1 (MQ=255) cGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTCCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGca > 1:1105786/1‑100 (MQ=255) aGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGcaca < 2:1444866/100‑1 (MQ=255) gCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACaa > 1:600843/1‑100 (MQ=255) cAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCATGGCTGCCACCATTCTGTTCCTGCAACCACGCGGGGcacca > 2:1094155/1‑97 (MQ=255) cAGACAGTAAAAGGACATATTCGCAATCTGCTGCGTAACCTCAATCTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAAt < 2:1070705/100‑1 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaa > 1:1434005/1‑100 (MQ=255) gACAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa > 2:979262/1‑99 (MQ=255) aCAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaaa > 2:1201864/1‑100 (MQ=255) cAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAAt < 2:599025/96‑1 (MQ=255) cAGTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATaaaa < 1:1270584/100‑1 (MQ=255) gTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAAt > 2:1384834/1‑100 (MQ=255) gTAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAAt > 1:875114/1‑100 (MQ=255) tAAAAGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATa < 1:1003004/100‑1 (MQ=255) aaaaGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAg < 1:1121126/100‑1 (MQ=255) aaGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGcc < 1:69694/100‑1 (MQ=255) aGTACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGccc > 1:1341434/1‑100 (MQ=255) tACATATTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGa < 1:1240734/100‑1 (MQ=255) atatTCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATgg < 1:321533/100‑1 (MQ=255) tCGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGAttt > 2:451891/1‑100 (MQ=255) cGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTa < 1:216414/100‑1 (MQ=255) cGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTa < 1:1431807/100‑1 (MQ=255) cGCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTa > 2:33969/1‑100 (MQ=255) gCAATCTGCTGCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTat < 2:1070349/100‑1 (MQ=255) ctgctgCGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTACCATCg > 2:537166/1‑100 (MQ=255) tgctgcGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCgg < 1:1299807/100‑1 (MQ=255) ctgcGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGc > 1:1560379/1‑100 (MQ=255) gcGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTg > 2:1444277/1‑100 (MQ=255) cGTAACCTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAACAATAGCCCGATGGATTTATCATCGGGCTga < 2:448200/100‑1 (MQ=255) gTAACCTCATTGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCTGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTgag < 2:313029/100‑1 (MQ=255) | ACGAGCTGGCACAGGGGCTGTCAAATAAACAGATTGCCTCGGTGTTGAATATTTCCGAGCAGACAGTAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAG > NC_000913/2290987‑2291179 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |