| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,352,993 | T→A | I116N (ATC→AAC) | glpA → | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,352,993 | 0 | T | A | 94.1% | 161.5 / 2.5 | 51 | I116N (ATC→AAC) | glpA | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (25/23); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01 | |||||||||||
TGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACC > NC_000913/2352899‑2353087 | tGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAgcg < 2:1393257/100‑1 (MQ=255) gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAg > 1:540713/1‑100 (MQ=255) ccaaccaaCGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCta > 2:9009/1‑100 (MQ=255) ccaaccaaCGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCta < 1:789299/100‑1 (MQ=255) caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCCCTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtat > 2:959997/1‑100 (MQ=255) caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtat > 1:165986/1‑100 (MQ=255) aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata > 2:578233/1‑100 (MQ=255) acGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGAccc < 2:1168233/100‑1 (MQ=255) acGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGAccc < 2:1167142/100‑1 (MQ=255) cTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgcagc < 2:991864/100‑1 (MQ=255) ttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgc < 1:123955/100‑1 (MQ=255) tCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg < 1:1327155/100‑1 (MQ=255) aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa > 1:849504/1‑100 (MQ=255) cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt > 2:1298828/1‑100 (MQ=255) cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt > 1:190402/1‑100 (MQ=255) ccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt > 1:1053882/1‑100 (MQ=255) gCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATc > 1:754593/1‑100 (MQ=255) ccGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCg > 1:1297811/1‑100 (MQ=255) cgcaaGACGCCCTGTCCTGCCAGGCCACATTGAGTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATc < 2:1370540/95‑1 (MQ=255) ggAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGTGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa < 2:1100538/100‑1 (MQ=255) ggAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa > 1:1004092/1‑100 (MQ=255) ggAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa > 1:1215108/1‑100 (MQ=255) ggAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa > 1:692349/1‑100 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAc < 1:1364739/100‑1 (MQ=255) aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc < 2:540710/100‑1 (MQ=255) gACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc > 2:1352465/1‑98 (MQ=255) gACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCg > 2:377531/1‑100 (MQ=255) gACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCg < 1:1562573/100‑1 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt < 1:1155692/100‑1 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt > 1:175619/1‑100 (MQ=255) tctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTaa > 2:796883/1‑100 (MQ=255) ctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAc > 1:1178863/1‑100 (MQ=255) ccTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGA‑CCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg < 2:1149609/100‑1 (MQ=255) cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg > 1:151507/1‑100 (MQ=255) cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg > 2:1305807/1‑100 (MQ=255) cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg < 1:1372705/100‑1 (MQ=255) cAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCAc < 2:1053875/100‑1 (MQ=255) cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATCATCGAACCTGCCGTTAACCCGGCACTGATTgg > 2:1162027/1‑100 (MQ=255) ttttATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgc > 1:910110/1‑100 (MQ=255) aTTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg < 1:1298837/100‑1 (MQ=255) aTTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg < 1:737054/100‑1 (MQ=255) cgcgCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa > 2:1048927/1‑100 (MQ=255) gCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTcc < 2:407905/100‑1 (MQ=255) cGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCg < 2:920361/100‑1 (MQ=255) cGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCg < 2:921120/100‑1 (MQ=255) gatctagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCgg < 2:173948/95‑1 (MQ=255) gaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg > 2:562265/1‑100 (MQ=255) gaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg < 2:692346/100‑1 (MQ=255) aagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATgg < 1:1129214/100‑1 (MQ=255) agCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGagatc > 1:173948/1‑96 (MQ=255) ggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCAcc < 1:208947/98‑1 (MQ=255) | TGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACC > NC_000913/2352899‑2353087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |