| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 236,541 | C→T | R159* (CGA→TGA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 236,541 | 0 | C | T | 96.4% | 183.0 / ‑3.0 | 56 | R159* (CGA→TGA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (29/25); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAG > NC_000913/236447‑236637 | cACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg < 2:199669/100‑1 (MQ=255) gATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGc > 1:654523/1‑100 (MQ=255) gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg > 1:202646/1‑100 (MQ=255) ccTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCATCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg < 2:167566/99‑1 (MQ=255) ccTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACggg > 1:133171/1‑100 (MQ=255) tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa < 1:128150/100‑1 (MQ=255) gCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAt > 1:195937/1‑100 (MQ=255) tGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACt < 1:257902/100‑1 (MQ=255) ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAACTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc > 2:1008406/1‑100 (MQ=255) ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc < 2:153398/100‑1 (MQ=255) ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc < 1:517890/100‑1 (MQ=255) ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc > 2:894488/1‑100 (MQ=255) aTGTTTCCCGCTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAGCGCTGCACGGGGCATTACTCGATGCCCAGa < 2:1031356/100‑1 (MQ=255) tGTTTCCCGATAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGAt > 1:300815/1‑100 (MQ=255) ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt > 1:797515/1‑100 (MQ=255) tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt > 2:84779/1‑100 (MQ=255) ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc > 1:917552/1‑100 (MQ=255) cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg > 2:607601/1‑100 (MQ=255) cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg < 2:990479/100‑1 (MQ=255) ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg > 1:1160189/1‑100 (MQ=255) aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg < 2:821232/100‑1 (MQ=255) ngcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt > 1:844378/2‑100 (MQ=255) gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt < 1:139596/100‑1 (MQ=255) gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt > 2:52665/1‑100 (MQ=255) gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt > 2:1382457/1‑100 (MQ=255) gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa < 2:590776/100‑1 (MQ=255) gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa < 1:1430360/100‑1 (MQ=255) aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATc < 1:975167/100‑1 (MQ=255) aGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTgg < 2:930541/100‑1 (MQ=255) gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc < 1:728126/100‑1 (MQ=255) ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg > 1:151755/1‑100 (MQ=255) gATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGa > 1:676711/1‑100 (MQ=255) tGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc < 2:1097580/100‑1 (MQ=255) cGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg < 1:1382467/100‑1 (MQ=255) gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt < 2:202645/100‑1 (MQ=255) aTGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt < 2:1047179/100‑1 (MQ=255) gCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa > 2:541117/1‑100 (MQ=255) cGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt > 2:271915/1‑100 (MQ=255) gCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc > 1:866434/1‑100 (MQ=255) gCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc < 1:607604/100‑1 (MQ=255) tACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGa > 2:814316/1‑100 (MQ=255) aCGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt < 1:994289/100‑1 (MQ=255) gAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg < 1:424238/100‑1 (MQ=255) aaataaatAACAGTAAATGAACGCTGCACGGGGCCATACGCGAGGCCCAGATCCTTGCGGAAGTTGATCTGGCGATGACCGGTGGTCAAACGTCGATgtc < 2:147706/100‑3 (MQ=255) aGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtttt < 2:1353839/100‑1 (MQ=255) tAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg < 1:84779/100‑1 (MQ=255) tAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg < 1:865478/100‑1 (MQ=255) tAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg < 1:866049/100‑1 (MQ=255) tAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg > 1:256157/1‑100 (MQ=255) aaCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg > 2:1243969/1‑99 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg > 2:1414412/1‑100 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg > 1:1389093/1‑100 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg > 2:1192371/1‑100 (MQ=255) aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg > 2:528891/1‑100 (MQ=255) gTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa < 1:1414422/100‑1 (MQ=255) aaaTGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg > 2:395235/1‑100 (MQ=255) aaaTGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg > 2:1288509/1‑100 (MQ=255) | CACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAG > NC_000913/236447‑236637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |