| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,452,339 | A→G | intergenic (‑3/+17) | yfcS ← / ← yfcU | putative periplasmic pilin chaperone/pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,452,339 | 0 | A | G | 97.5% | 282.2 / ‑2.3 | 81 | intergenic (‑3/+17) | yfcS/yfcU | putative periplasmic pilin chaperone/pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (38/41); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATG > NC_000913/2452242‑2452434 | ggCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATc < 1:608000/100‑1 (MQ=255)ggCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATc < 1:285632/100‑1 (MQ=255) gCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCa > 1:1121007/1‑100 (MQ=255) gCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCa > 1:1441743/1‑100 (MQ=255) gCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCa > 1:1315754/1‑100 (MQ=255) cGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCAt > 2:1227119/1‑100 (MQ=255) cGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCAt > 1:170654/1‑100 (MQ=255) tACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTcc < 1:1313109/100‑1 (MQ=255) aCAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCt < 1:625364/100‑1 (MQ=255) aCAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCt > 2:238971/1‑100 (MQ=255) aCAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCt < 1:792261/100‑1 (MQ=255) gACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTaa > 1:658223/1‑100 (MQ=255) cAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACg < 2:608901/100‑1 (MQ=255) cAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACg > 2:1125514/1‑100 (MQ=255) aGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGt > 2:879220/1‑100 (MQ=255) gggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGtt > 1:708083/1‑100 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTa > 1:1516489/1‑100 (MQ=255) cAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTAtt > 1:1439138/1‑100 (MQ=255) aCAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGt < 1:124220/100‑1 (MQ=255) cAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGtt < 2:1389056/100‑1 (MQ=255) cAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGtt > 2:879709/1‑100 (MQ=255) cAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGtt > 2:1356049/1‑100 (MQ=255) aaCTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAg < 1:521099/100‑1 (MQ=255) tGGTAGCGGTAAGCAGTAACACCAGCGTCATAGCGCCCAATTTGGTAGAGCCATGTAAATCAGACATGGGTCATTCCTTAAGCTCGTTATTGGTCAGCt < 2:261339/99‑1 (MQ=255) ggTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTga > 1:29669/1‑100 (MQ=255) ggTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTga > 2:899664/1‑100 (MQ=255) gcgcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaaca > 2:285721/1‑100 (MQ=255) cgcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaacag < 2:1441733/100‑1 (MQ=255) gcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaacaga > 1:1018555/1‑100 (MQ=255) tAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaacagaaa < 2:223457/100‑1 (MQ=255) aGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaacagaaaca > 1:631675/1‑100 (MQ=255) gaaGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGaaacagaaacag > 1:1057110/3‑100 (MQ=255) cAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgg > 1:695761/1‑100 (MQ=255) gTAACGCCATCGTCATAGCACCCAATTTGGCAGAGCAAATTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgggg < 2:1429475/100‑1 (MQ=255) gTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgggg < 1:79201/100‑1 (MQ=255) gTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgggg > 2:1423791/1‑100 (MQ=255) tAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAggggg < 2:1439128/100‑1 (MQ=255) tAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAAACAGACATGGGTCATTCCTTACTCACGTTATTGGTTAGCTGAAACAGAAACAggggg < 1:392813/100‑1 (MQ=255) aaCGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGa > 1:608106/1‑100 (MQ=255) ccAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCgg < 2:836439/100‑1 (MQ=255) ttccgatctAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggt < 2:289867/92‑1 (MQ=255) gCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtgg > 1:1559376/1‑100 (MQ=255) cGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggg < 2:232197/100‑1 (MQ=255) cGTCATAACACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggg < 1:821247/100‑1 (MQ=255) gTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtgggt > 1:875825/1‑100 (MQ=255) tCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtgggtg < 2:1567308/100‑1 (MQ=255) tCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtgggtg < 1:682311/100‑1 (MQ=255) aTAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTc < 1:1054469/100‑1 (MQ=255) aGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtagatcggaa > 1:289868/1‑91 (MQ=255) aGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCAc < 1:1227126/100‑1 (MQ=255) aGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCAc < 1:101078/100‑1 (MQ=255) gCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCAcc < 2:170654/100‑1 (MQ=255) gCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCAcc > 1:1185385/1‑100 (MQ=255) cACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCt > 2:1419641/1‑100 (MQ=255) cAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTg < 2:1161132/100‑1 (MQ=255) cAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTg < 2:29669/100‑1 (MQ=255) cAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTg < 1:1423801/100‑1 (MQ=255) tttGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGt < 1:251912/100‑1 (MQ=255) ttGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 2:564614/100‑1 (MQ=255) tGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCt < 2:695758/100‑1 (MQ=255) gCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGc > 2:1097019/1‑100 (MQ=255) nagagCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCt > 2:71561/2‑100 (MQ=255) cAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCt > 2:71813/1‑100 (MQ=255) cAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCt < 1:1356058/100‑1 (MQ=255) cAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCt > 2:486004/1‑100 (MQ=255) agagCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTc > 1:938832/1‑100 (MQ=255) gagCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTcc < 1:1242109/100‑1 (MQ=255) gagCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTcc > 2:1403453/1‑100 (MQ=255) agCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAACCAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTcc < 1:704566/99‑1 (MQ=255) agCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCt > 2:327450/1‑100 (MQ=255) gCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTcc < 1:626517/98‑1 (MQ=255) agaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGaa > 1:1062789/3‑100 (MQ=255) aaaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGaa > 2:1140633/1‑100 (MQ=255) aaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAAt < 2:986718/100‑1 (MQ=255) aaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAAt > 2:824662/1‑100 (MQ=255) aaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAAt < 2:81301/100‑1 (MQ=255) aaGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAAt < 2:1315745/100‑1 (MQ=255) aGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATg < 1:1125521/100‑1 (MQ=255) gTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGa < 2:209129/100‑1 (MQ=255) aaaTCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACa < 2:1185378/100‑1 (MQ=255) aTGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATg < 1:674958/100‑1 (MQ=255) | GGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATG > NC_000913/2452242‑2452434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |