| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,540,239 | T→A | L26Q (CTG→CAG) | feoA → | ferrous iron transporter, protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,540,239 | 0 | T | A | 94.3% | 174.7 / 2.3 | 53 | L26Q (CTG→CAG) | feoA | ferrous iron transporter, protein A |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (33/17); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
ATGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCT > NC_000913/3540142‑3540335 | aTGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGc < 2:1397937/100‑1 (MQ=255) agaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCttt > 2:820359/1‑100 (MQ=255) agaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCttt > 2:805615/1‑100 (MQ=255) gaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTtc > 1:1522606/1‑100 (MQ=255) gaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGAGAAATCAGCCCGGCATATCGCCAAAAACAGCTTtc > 1:163413/1‑100 (MQ=255) aaCAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTTGAAAATCACTGGCTTTTCCCATGAAAACAGCCCGGCATATCGCCAAAAACAGCTTtctc > 1:470647/1‑100 (MQ=255) aCAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTctct > 2:1461289/1‑100 (MQ=255) cAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCtt > 1:1472211/1‑100 (MQ=255) gTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGc > 2:298747/1‑100 (MQ=255) tAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCa > 1:1208560/1‑100 (MQ=255) ccTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTAc > 1:65269/1‑100 (MQ=255) ccTAGGAAATACACTCCAGATACTGCGTGGAAAATCAATGTCTTTTCCCGTGAATTCAGCTCGAAAAAAAAAAAAAAACAGCTTTCTCTTGGCATGTTAc < 2:223348/100‑1 (MQ=255) tATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCt > 1:411768/1‑100 (MQ=255) tGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTgg > 1:1037849/1‑100 (MQ=255) gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGc < 2:1037842/100‑1 (MQ=255) gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGc > 1:73823/1‑100 (MQ=255) gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGc < 1:593097/100‑1 (MQ=255) gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCAAAAAACAGCTTTCTCTTGGCATGTTACCTGGc < 2:523631/100‑1 (MQ=255) acTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCtt < 1:1026062/100‑1 (MQ=255) tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCtttt < 1:361286/100‑1 (MQ=255) ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa > 2:1250565/1‑100 (MQ=255) ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa < 1:575038/100‑1 (MQ=255) ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa > 1:1201010/1‑100 (MQ=255) gATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAAtg > 2:84730/1‑100 (MQ=255) tACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAGACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAAtgtg > 2:1006169/1‑100 (MQ=255) cGTGGAAAATCACTCGCTGTCCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGATATCTCTTGGCATGTTACCTGGCTCCTCTTTTAATctggt < 2:861285/97‑1 (MQ=255) aaaaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCg < 1:1122743/100‑1 (MQ=255) aTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTc > 2:1305319/1‑100 (MQ=255) tCACTTGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTcc < 1:1478905/100‑1 (MQ=255) aCTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCAc > 2:562396/1‑100 (MQ=255) ggCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCg > 1:457745/1‑100 (MQ=255) ccgatctGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAc < 1:267610/94‑1 (MQ=255) cccGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAccc > 1:1394768/1‑100 (MQ=255) ccGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAcccc > 1:397616/1‑100 (MQ=255) cGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCGCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCa > 2:966718/1‑100 (MQ=255) gAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAcagatcgg > 2:267609/1‑93 (MQ=255) aaTCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCat < 1:879749/100‑1 (MQ=255) aTCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCata < 1:1298853/100‑1 (MQ=255) gCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGa > 1:51783/1‑100 (MQ=255) cGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAc < 1:385722/100‑1 (MQ=255) gCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAccc > 1:996409/1‑100 (MQ=255) gCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAccc < 1:558469/100‑1 (MQ=255) tatCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCcgtc > 2:779590/1‑100 (MQ=255) cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg < 1:779594/100‑1 (MQ=255) cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg > 2:595864/1‑100 (MQ=255) cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg > 1:423733/1‑100 (MQ=255) gCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtgt > 2:81187/1‑100 (MQ=255) gCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtgt > 1:634473/1‑100 (MQ=255) ccAAAATCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTGTAATGTGGTGCGCGTCGCTCCACTCGGCGTCCCCATTCATATCGAAACCCGTCGtgtg < 2:98945/100‑1 (MQ=255) ccAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGtgtg > 2:1212383/1‑100 (MQ=255) aaaaaCTGCTTTCCCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGtgtg > 2:1225166/1‑98 (MQ=255) aaaaCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGATGCGCGTCGCTCCACTCGGCGACCCCATTCATATCAAAACCCGTCGTGTGa > 2:98766/1‑98 (MQ=255) aaCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCt > 2:1422117/1‑100 (MQ=255) | ATGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCT > NC_000913/3540142‑3540335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |