| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 343,467 | A→C | H195P (CAC→CCC) | yahK → | broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 343,467 | 0 | A | C | 95.5% | 151.4 / ‑3.7 | 44 | H195P (CAC→CCC) | yahK | broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (21/21); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACG > NC_000913/343373‑343560 | tATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGa > 2:301365/1‑100 (MQ=255) aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGAt < 2:208638/100‑1 (MQ=255) cgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGc < 2:808005/100‑1 (MQ=255) cGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACAtg > 1:553565/1‑100 (MQ=255) gTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACAtgt > 2:456918/1‑100 (MQ=255) cACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGCGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGTGGCGCATgtgg < 2:827094/100‑1 (MQ=255) tGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGtggtgg < 1:118904/100‑1 (MQ=255) gCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCa < 2:274017/100‑1 (MQ=255) cAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCAt > 1:1119666/1‑100 (MQ=255) cAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCAt > 2:137985/1‑100 (MQ=255) gccgggccggGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTa < 2:339613/100‑1 (MQ=255) cgggccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTAcc < 1:389606/100‑1 (MQ=255) aaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACACGTGGTGGCATTTACCACTTCTGAGGCaa > 2:1136115/1‑100 (MQ=255) aaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCaaa < 2:1539392/100‑1 (MQ=255) tGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTACGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAAcg < 1:292875/100‑1 (MQ=255) gCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGa < 1:456920/100‑1 (MQ=255) gCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGa < 2:590377/100‑1 (MQ=255) tGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGc > 1:840288/1‑100 (MQ=255) gTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCgg > 1:1004170/1‑100 (MQ=255) cGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCa > 1:1371394/1‑100 (MQ=255) ggCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaa > 1:1490692/1‑100 (MQ=255) gCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaa > 2:607002/1‑100 (MQ=255) cATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa > 1:1125152/1‑100 (MQ=255) cATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa > 2:679033/1‑100 (MQ=255) tCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGc > 2:365084/1‑100 (MQ=255) ggTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTgg < 2:477262/100‑1 (MQ=255) cTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAACAGCCCTggggg > 2:713943/1‑100 (MQ=255) cTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTggggg > 1:494812/1‑100 (MQ=255) gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:454227/100‑1 (MQ=255) gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:454215/100‑1 (MQ=255) gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:254849/100‑1 (MQ=255) cATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg < 2:847385/100‑1 (MQ=255) cATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg < 2:893714/100‑1 (MQ=255) atGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAg < 1:607005/100‑1 (MQ=255) atGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAg < 1:1339752/100‑1 (MQ=255) tGGGGATTAAGCTGGCTCACGGGGTGGGGGGACATGTGGTGGCGTTTACAACTTCTGAGGCAAAACACGAAGCGGGAAAAGGCCGGGGGGCCGAtcgag > 2:151235/1‑95 (MQ=255) tGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgt > 2:479746/1‑100 (MQ=255) ggATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgt > 1:1274127/1‑100 (MQ=255) gATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgtt < 2:1180096/100‑1 (MQ=255) gATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgt < 2:1175213/99‑1 (MQ=255) aTTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTa > 1:1320032/1‑100 (MQ=255) aGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTc > 1:420690/1‑100 (MQ=255) cTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCAc < 2:175104/100‑1 (MQ=255) tGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACg > 2:329593/1‑100 (MQ=255) | TATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACG > NC_000913/343373‑343560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |