Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,648,042	T→C	intergenic (‑254/‑486)	dinQ ← / → arsR	UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,042	0	T	C	100.0%	195.2 / NA	53	intergenic (‑254/‑486)	dinQ/arsR	UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/30);  total (23/30)

ATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGG  >  NC_000913/3647946‑3648137                                                                                                 |                                                                                                aTGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATg                                                                                              <  1:49193/100‑1 (MQ=255)   gcgcATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGt                                                                                            <  2:1527153/100‑1 (MQ=255)       aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                        <  2:574882/100‑1 (MQ=255)       aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                        <  1:945373/100‑1 (MQ=255)        ttGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAg                                                                                       <  1:1004381/100‑1 (MQ=255)        ttGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAg                                                                                       <  2:369735/100‑1 (MQ=255)          gCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCa                                                                                     >  1:407517/1‑100 (MQ=255)          gCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCa                                                                                     >  2:1542870/1‑100 (MQ=255)            cAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCAtt                                                                                   >  1:91845/1‑100 (MQ=255)            cAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCAtt                                                                                   <  1:1445016/100‑1 (MQ=255)              gagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGa                                                                                 >  2:549994/1‑100 (MQ=255)                gTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATg                                                                               >  2:1266686/1‑100 (MQ=255)                gTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATg                                                                               >  2:1170879/1‑100 (MQ=255)                  gAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCg                                                                             <  2:448134/100‑1 (MQ=255)                    aaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGtt                                                                           <  1:539739/100‑1 (MQ=255)                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          <  1:1142134/100‑1 (MQ=255)                        gCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgat                                                                       <  2:1135360/100‑1 (MQ=255)                         ccTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatg                                                                      >  1:745460/1‑100 (MQ=255)                         ccTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatg                                                                      >  1:1357684/1‑100 (MQ=255)                         ccTCAGAGCTAACTAGATAACCATACAGTACATGCAGGTTATATAACCAGCACGTACCTGCAAGAGTATCAGCATGGTATTAGTAGTGATGCGTTAgatc                                                                      <  2:1197404/100‑2 (MQ=255)                             agagCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATgg                                                                  <  1:1350149/100‑1 (MQ=255)                                gCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               >  1:1531279/1‑100 (MQ=255)                                   aaCTGGATACTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtc                                                             <  1:464598/99‑1 (MQ=255)                                   aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtct                                                            <  2:640510/100‑1 (MQ=255)                                    aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtctc                                                           >  1:1193787/1‑100 (MQ=255)                                     cTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCa                                                          >  1:10647/1‑100 (MQ=255)                                     cTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCa                                                          >  1:110647/1‑100 (MQ=255)                                     cTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCa                                                          >  1:110719/1‑100 (MQ=255)                                        gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTc                                                       <  2:1333423/100‑1 (MQ=255)                                           aaTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAg                                                    <  2:61756/100‑1 (MQ=255)                                            aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                   >  2:612958/1‑100 (MQ=255)                                            aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                   >  1:606480/1‑100 (MQ=255)                                             tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTc                                                  <  2:110719/100‑1 (MQ=255)                                             tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTc                                                  <  2:10647/100‑1 (MQ=255)                                             tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTc                                                  <  1:1527867/100‑1 (MQ=255)                                             tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAATc                                                  <  2:110647/100‑1 (MQ=255)                                               aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                <  2:1193824/100‑1 (MQ=255)                                               aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                <  2:112560/100‑1 (MQ=255)                                                tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCaga                                               <  1:1426970/100‑1 (MQ=255)                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCGTGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      >  1:110905/1‑100 (MQ=255)                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      <  2:123980/100‑1 (MQ=255)                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      >  2:264560/1‑100 (MQ=255)                                                             ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAg                                  >  1:1132570/1‑100 (MQ=255)                                                                  taAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCt                              >  2:480481/1‑99 (MQ=255)                                                                   aaaaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTgg                            >  2:1466535/1‑100 (MQ=255)                                                                   aaaaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTgg                            >  2:1056399/1‑100 (MQ=255)                                                                    aaaCCAGCACGTCCTTGCAATACTTTCAGCATGCTATTAGCATTGATGCTTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTgg                            <  2:1359550/99‑1 (MQ=38)                                                                      aCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGaaa                         >  1:1205252/1‑100 (MQ=255)                                                                       ccAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAg                        <  1:1319515/100‑1 (MQ=255)                                                                              gTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTaaaaaa                 <  1:43575/100‑1 (MQ=255)                                                                                ccTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAcc               >  2:292105/1‑100 (MQ=255)                                                                                           tttCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTc    <  1:521704/100‑1 (MQ=255)                                                                                             tCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCgg  <  1:1170886/100‑1 (MQ=255)                                                                                                 |                                                                                                ATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGG  >  NC_000913/3647946‑3648137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑